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TRF1 antibody (Telomeric Repeat Binding Factor (NIMA-Interacting) 1)

Details for Product anti-TERF1 Antibody No. ABIN249348, Supplier: Login to see New
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Arabidopsis thaliana, Rice (Oryza sativa)
(114), (29), (18), (2), (2), (1), (1), (1), (1)
(75), (23), (18)
This TRF1 antibody is un-conjugated
(3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunocytochemistry (ICC), Western Blotting (WB)
(85), (63), (18), (13), (12), (11), (9), (8), (2), (1), (1)
Pubmed 4 references available
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Quantity 200 μg
Shipping to United States ( )
Immunogen KLH-conjugated mixture of two synthetic peptides derived from AtPIN2 sequence At5g57090
Isotype IgY
Cross-Reactivity (Details) Kinetic and allosteric properties of the plant cytosolic FBPase are remarkably similar to the mammalian and yeast FBPase, but differ greatly from those of the chloroplastic FBPase. The antibody could detect FBPase from the human COS-7cell line transfected with FBP1 expressing vector.
Characteristics Expected / apparent Molecular Weight of the Antigene: 69 kDa
Purification affinity purified
Alternative Name PIN2 (TERF1 Antibody Abstract)
Background AGI Code: At5g57090
Arabidopsis thaliana auxin efflux carrier component AtPIN2 encoded by the AtPIN2 gene (also known as EIR1 and AGR1). AtPIN proteins are asymmetrically localized within plant plasma membranes and mediate polar auxin transport. AtPIN2 is a key regulator of the response of Arabidopsis roots to gravity.
Molecular Weight 69 kDa
UniProt Q9LU77
Pathways Cell Division Cycle, Telomere Maintenance
Application Notes Recommended Dilution: 1 : 2000 - 1 : 4000 (WB).
This antibody will only work in western blot if the isolated first micrometers of theroot tip from seedlings is used. This is the place where PIN2 protein is mos tstrongly expressed (Willige et al. 2011). And the antibody also works with the whole root extracts when the microsomal membrane fractions are used for western blotting. Latter will eliminate the crossreacting bands.

5% normal donkey serum (Jackson ImmunoResearch, West Grove, PA) in MTSB was used for blocking and the secondary antibody was donkey anti-chicken TRITC-coupled 1:250 (Jackson ImmunoResearch). Specific PIN2 signal in epidermis, lateral root cap and cortex cells. Cross reaction with another (PIN) protein in the QC and first columella layers in the in the (Men et al. 2008, Nat. Cell Biol. 10, Suppl. Information).

Restrictions For Research Use only
Format Lyophilized
Buffer PBS pH 8.5+ 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Storage -20 °C
Storage Comment Store lyophilized/reconstituted at -20 °C for up to a year.
Expiry Date 12 months
Background publications Cho, Yoo, Zhang et al.: "The rice narrow leaf2 and narrow leaf3 loci encode WUSCHEL-related homeobox 3A (OsWOX3A) and function in leaf, spikelet, tiller and lateral root development." in: The New phytologist, Vol. 198, Issue 4, pp. 1071-84, 2013 (PubMed).

Drdová, Synek, Pečenková et al.: "The exocyst complex contributes to PIN auxin efflux carrier recycling and polar auxin transport in Arabidopsis." in: The Plant journal : for cell and molecular biology, Vol. 73, Issue 5, pp. 709-19, 2013 (PubMed).

Willige, Isono, Richter et al.: "Gibberellin regulates PIN-FORMED abundance and is required for auxin transport-dependent growth and development in Arabidopsis thaliana." in: The Plant cell, Vol. 23, Issue 6, pp. 2184-95, 2011 (PubMed).

Men, Boutté, Ikeda et al.: "Sterol-dependent endocytosis mediates post-cytokinetic acquisition of PIN2 auxin efflux carrier polarity." in: Nature cell biology, Vol. 10, Issue 2, pp. 237-44, 2008 (PubMed).

Catalog No. ABIN249348

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