alpha-1-Inhibitor 3 (A1I3) antibody

Antigen: alpha-1-Inhibitor 3 (A1I3)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN249360

Additional Information

Immunogen: purified rat alpha-1-inhibitor 3 P14046

Format: Lyophilized

Description: Rat alpha-1-inhibitor 3 (A1i3) is a proteinase inhibitor in rat plasma. It belongs tothe same superfamily as human alpha-2-macroglobulin (a2M). Alternative names:Alpha-1-inhibitor 3 variant II antibody, Alpha-1-inhibitor III antibody

Specificity: Predicted reactivity: rat.

Application Details

Application Notes: Recommended Dilution 1: 1000 with standard ECL (WB).

Buffer: total IgG in PBS pH 7.4

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

Experimental conditions: Blotting with rabbit anti-rat a1inhibitor-3. Ten ml of rat plasma, diluted 200 times with PBS, and 20 ng purified rat a1inhibitor-3 were separated on a 9% polyacrylamide gel, transferred to a polyvinylidene difluoride membrane, blocked, incubated 2 h at RT with anti-a1inhibitor1 mg/ml. washed and then incubated for 30 min with swine anti-rabbit IgG-HRP and washed. The membrane was developed using the ECLsystem.