Complement Component C4 (Co4) antibody

Antigen: Complement Component C4 (Co4)

Clonality: Polyclonal

Host: Chicken

Reactivity: Human

Application: Western Blotting (WB), Immunodiffusion (ID)

Catalog no.: ABIN249404

Additional Information

Immunogen: full length native protein P0C0L4 purified from human plasma

Format: Liquid

Description: Complement component C4 consists of three polypeptide chains (alpha,molecular weight 95 kDa, beta, molecular weight 75 kDa and gamma, molecularweight 33 kDa) and is usually present in serum at 200-600 mg/L. It is a protein ofthe classical complement pathway. When activated by C1s, peptide C4a isreleased and the generated C4b binds C2 which is in turn cleaved by C1s toproduce the classical pathway C3 convertase, namely C4b2a. C4b also hasopsonin activity and binds to macrophages and neutrophils via their CR1 receptor.

Specificity: Predicted reactivity: human.

Application Details

Application Notes: Recommended Dilution 1: 1000 with standard ECL (WB).

Purification: affinity purified IgY on human C4 agarose

Buffer: Affinity purified IgY at 1 mg/ml in 0.15M sodium chloride,0.02M sodium phosphate, 0.1% sodium azide pH 7.2

Storage: store at 4°C, make aliquots to avoid working with a stock. Please, remember tospin tubes briefly prior to opening them to avoid any losses that might occur fromliquid material adhering to the cap or sides of the tubes.

Restrictions: For Research Use only