Tumor Necrosis Factor (TNF) antibody (Biotin)

Antigen: Tumor Necrosis Factor (TNF)

Synonyms: DIF, TNFA, TNFSF2, TNF-alpha, B12, B61, EDP1, MGC2317, Edp1, Edp-1, Tnfip1, Tnfa, RATTNF, MGC124630, zgc:55302, wu:fi06b02, TNFAIP1, TNF, tnf, DKFZp468A2328, Tnfsf1a, TNFalpha, MGC151434, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, TNF-a, tnfsf2, tnf-alpha, Cachectin

Clonality: Polyclonal

Host: Chicken

Reactivity: Human

Conjugate: Biotin

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN249407

Additional Information

Alternative name: Tumor necrosis factor alpha (TNFa)

Immunogen: purified full length recombinant protein P01375

Format: Liquid

Description: Tumor Necrosis Factor alpha (TNF alpha) is a protein secreted bylipopolysaccharide stimulated macrophages, and causes tumor necrosis wheninjected into tumour bearing mice. TNF alpha is believed to mediate pathogenicshock and tissue injury associated with endotoxemia. TNF alpha exists as amultimer of two, three, or five noncovalently linked units, but shows a single 17kDa band following SDS PAGE under non reducing conditions. TNF alpha isclosely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin),sharing the same receptors and cellular actions. TNF alpha causes cytolysis orcytostasis of certain transformed cells, being synergistic with interferon gamma inits cytotoxicity. Although it has little effect on many cultured normal human cells,TNF alpha appears to be directly toxic to vascular endothelial cells. Other actionsof TNF alpha include stimulating growth of human fibroblasts and other cell lines,activating polymorphonuclear neutrophils and osteoclasts, and induction ofinterleukin 1, prostaglandin E2 and collagenase

Specificity: Predicted reactivity: cat, dog, horse, pig, primates.

Synonyms: DIF, TNFA, TNFSF2, TNF-alpha, B12, B61, EDP1, MGC2317, Edp1, Edp-1, Tnfip1, Tnfa, RATTNF, MGC124630, zgc:55302, wu:fi06b02, TNFAIP1, TNF, tnf, DKFZp468A2328, Tnfsf1a, TNFalpha, MGC151434, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, TNF-a, tnfsf2, tnf-alpha, Cachectin

Application Details

Application Notes: Recommended Dilution 1: 5000 with standard ECL (WB). Additional Information: the IgY fraction is isolated by a two-step PEG precipitation procedure followed byammonium sulphate precipitation, labelled with biotin. Total IgY concentration for this antibody is 15 mg/ml .

Buffer: Affinity purified IgY in 0.15M sodium chloride, 0.02M sodiumphosphate, 0.1 % sodium azide pH 7.2

Storage: store at 4°C, make aliquots to avoid working with a stock. Please, remember tospin tubes briefly prior to opening them to avoid any losses that might occur fromliquid material adhering to the cap or sides of the tubes.

Restrictions: For Research Use only