Streptococcal Protein G antibody

Antigen: Streptococcal Protein G

Clonality: Polyclonal

Host: Chicken

Reactivity: Streptococcus

Application: Western Blotting (WB)

Catalog no.: ABIN249416

Additional Information

Immunogen: purified recombinant protein P06654 (lacking albumin binding region)

Format: Liquid

Description: Protein G is a bacterial protein derived from the cell wall of certain strains ofb-hemolytic Streptococcci. It binds with high affinity to the Fc portion of variousclasses and subclasses of immunoglobulins from a variety of species. Protein Gbinds to all IgG subclasses from human, mouse and rat species. It also binds tototal IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G bindspreferentially to the Fc portion of IgG, but unlike Protein A, can also bind to theFab region, making it useful for purification of F(ab') fragments of IgG. Due to itsaffinity for the Fc region of many mammalian immunoglobulins, protein G isconsidered a universal reagent in biochemistry and immunology.

Specificity: Predicted reactivity: Streptococcus sp.

Application Details

Application Notes: Recommended Dilution 1: 1000 with standard ECL (WB).

Buffer: Affinity purified IgY at 1 mg/ml in 0.15M sodium chloride,0.02M sodium phosphate, 0.1 % sodium azide pH 7.2

Storage: store at 4°C, make aliquots to avoid working with a stock. Please, remember tospin tubes briefly prior to opening them to avoid any losses that might occur fromliquid material adhering to the cap or sides of the tubes.

Restrictions: For Research Use only