E74-Like Factor 3 (Ets Domain Transcription Factor, Epithelial-Specific) (ELF3) antibody

Antigen: E74-Like Factor 3 (Ets Domain Transcription Factor, Epithelial-Specific) (ELF3)

Synonyms: ERT, ESX, EPR-1, ESE-1, RGD1560743, ef2, zef2, ELF2, ELF3, ELF4, MGC139318, wu:fb06f04, DKFZp469F2239, ert, esx, epr-1, ese-1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN249576

Additional Information

Alternative name: ELF3 / ESE-1

Gene ID: 1999

NCBI Acession: NP_004424

Immunogen: Synthetic peptide SGWKEEEVLQSRN, representing the C-Terminus of the humanprotein (residues 359-371), according to NP_004424. Reference Sequence: NP_004424.

Cross-Reactivity: Human

Isotype: IgG

Description: ESE-1 (Epithelium-Specific Ets) is a novel, highly tissue-restricted member of the etstranscription factor/oncogene family, which has features distinct from those of any otherets-related factor. ESE-1 binds with high affinity to and transactivates the ets bindingsite in the promoter of the keratinocyte terminal differentiation marker gene, SPRR2A.ESE-1 is a critical regulator of epithelial cell differentiation. Alternate Names: anti-E74-like factor 3 (ets domain transcription factor epithelial-specific) antibody,anti-ELF3 antibody, anti-EPR-1 antibody, anti-ERT antibody, anti-ESE-1 antibody,anti-ESX antibody.

Specificity: Species Reactivity: Cross-reacts with Human. Not yet tested in other species.

Application Details

Application Notes: Suggested working dilutions: Western Blot 2.0 to 4.0 ug/ml. Positive Controls: NCI-H460 lysate.

Concentration: 0.5 mg/ml

Purification: Affinity Purified

Buffer: Tris saline containing [pH7.3] with 0.5% BSA. Preservative: 0.02% Na azide.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Images

ABIN249576 staining (3µg/ml) of NCI-H460 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Kohno, Okamoto, Ishibe et al.: "Expression of claudin7 is tightly associated with epithelial structures in synovial sarcomas and regulated by an Ets family transcription factor, ELF3." in: The Journal of biological chemistry, Vol. 281, Issue 50, pp. 38941-50, 2006 (PubMed).