OGG1 antibody (8-Oxoguanine DNA Glycosylase)

Details for Product anti-OGG1 Antibody No. ABIN249594, Supplier: Log in to see
Antigen
  • OGG1
  • zgc:158858
  • 8-oxoguanine-DNA glycosylase 1
  • ARABIDOPSIS 8-OXOGUANINE-DNA GLYCOSYLASE 1
  • ATOGG1
  • F8K7.14
  • F8K7_14
  • 19.m02329
  • DDBDRAFT_0184479
  • DDBDRAFT_0233024
  • DDB_0184479
  • DDB_0233024
  • ogg1
  • Tb04.1H19.930
  • HMMH
  • HOGG1
  • MUTM
  • OGH1
  • Mmh
  • 8-oxoguanine DNA glycosylase
  • 8-oxoguanine-DNA glycosylase 1
  • 8-OXOGUANINE DNA GLYCOSYLASE
  • 8-oxoguanine-DNA-glycosylase
  • 8-oxoguanine DNA-glycosylase
  • N-glycosylase/DNA lyase
  • 8-oxoguanine DNA-glycosylase 1
  • OGG1
  • ogg1
  • ECU08_0770
  • CAC2707
  • BBOV_I004290
  • Smp_074010.1
  • Tb927.4.2480
  • Ogg1
Alternatives
anti-Rat (Rattus) OGG1 antibody for ELISA
Reactivity
Human, Mouse (Murine), Primate, Rat (Rattus)
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12
8
2
2
2
1
1
Host
Rabbit
84
44
20
Clonality
Polyclonal
Conjugate
This OGG1 antibody is un-conjugated
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9
6
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
91
70
46
44
44
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38
17
11
5
2
Options
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Immunogen A peptide derived from human Ogg1 (within amino acids 1-100). [UniProt# O15527]
Purification Immunogen affinity purified
Alternative Name OGG1 (OGG1 Antibody Abstract)
Background Gene Symbol: OGG1
Molecular Weight Theoretical MW: 39 kDa
Gene ID 4968
UniProt O15527
Pathways DNA Damage Repair
Application Notes Western Blot 1:500-1:1000, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:100 - 1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Frozen 1:25-1:100This Ogg1 antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation Co-IP (PMID: 20956573), ELISA (PMID: 19506022) and Western Blot. In WB, it recognizes a band at ~39 kDa, representing Ogg1. In ICC/IF nuclear staining was observed in Hek293 cells. Flow data from customer review. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western blot Protocol for Ogg1 Antibody Western Blot Procedure
1. Run 50 µg of protein on a 4-20 % Tris-glycine mini-gel at 125V for 90 minutes.
. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes.
. Transfer protein to the membrane at 25V for 90 minutes.
. Allow membrane to air-dry.
. Block membrane with 1XPBS/3 % BSA for 1 hour at room temperature (23-27 degrees C).
. Wash membrane twice, for 5 minutes each, with 1XPBS/0.05 % Tween-20 (PBST).
. Incubate membrane with NB100-106 (anti-hOGG1), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Incubate membrane with goat anti-rabbit IgG-HRP, diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Detect cross-reacting proteins using Renaissance Chemiluminescence Reagent Plus kit from NEN Life Sciences.Immunohistochemistry Protocol for Ogg1 Antibody Immunohistochemistry - FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution:Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.Use within 4 hours of preparationB. Place slides in distilled water: 2 changes for 2 minutes each.III. Retrieve Epitopes:A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-OGG1 antibody (8-Oxoguanine DNA Glycosylase) (ABIN249594) anti-8-Oxoguanine DNA Glycosylase (OGG1) antibody
Western Blotting (WB) image for anti-OGG1 antibody (8-Oxoguanine DNA Glycosylase) (ABIN249594) anti-8-Oxoguanine DNA Glycosylase (OGG1) antibody (Image 2)
Western Blotting (WB) image for anti-OGG1 antibody (8-Oxoguanine DNA Glycosylase) (ABIN249594) 1, 5, 10 ng titration of human recombinant Ogg1 protein, detected by ABIN151034.
Immunoprecipitation (IP) image for anti-OGG1 antibody (8-Oxoguanine DNA Glycosylase) (ABIN249594) Immunoprecipitation: OGG1 Antibody [ABIN151034] - analysis of OGG1 in MRC5 whole cell...
Flow Cytometry (FACS) image for anti-OGG1 antibody (8-Oxoguanine DNA Glycosylase) (ABIN249594) Flow Cytometry: OGG1 Antibody [ABIN151034] - Baseline Ogg1 expression in human PBMC f...
Western Blotting (WB) image for anti-OGG1 antibody (8-Oxoguanine DNA Glycosylase) (ABIN249594) Western Blot: OGG1 Antibody [ABIN151034] - Analysis of Ogg1 recombinant protein using...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-OGG1 antibody (8-Oxoguanine DNA Glycosylase) (ABIN249594) Immunohistochemistry-Paraffin: OGG1 Antibody [ABIN151034] - Analysis of OGG1 in human...
Immunohistochemistry (IHC) image for anti-OGG1 antibody (8-Oxoguanine DNA Glycosylase) (ABIN249594) Immunohistochemistry: OGG1 Antibody [ABIN151034] - Staining of tonsil, germinal cente...
Immunofluorescence (IF) image for anti-OGG1 antibody (8-Oxoguanine DNA Glycosylase) (ABIN249594) Immunocytochemistry/Immunofluorescence: OGG1 Antibody [ABIN151034] - A431 cells were ...
Product cited in: Wang, Lai, Li, Chiang, Yu, Sakamoto, Tu, Hsieh, Huang, Chuang, Dai, Yu: "The Paradoxical Effects of Different Hepatitis C Viral Loads on Host DNA Damage and Repair Abilities." in: PLoS ONE, Vol. 12, Issue 1, pp. e0164281, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Moreli, Santos, Lorenzon-Ojea, Corrêa-Silva, Fortunato, Rocha, Rudge, Damasceno, Bevilacqua, Calderon: "Hyperglycemia Differentially Affects Maternal and Fetal DNA Integrity and DNA Damage Response." in: International journal of biological sciences, Vol. 12, Issue 4, pp. 466-77, 2016 (PubMed).

Liśkiewicz, Kasprowska, Wojakowska, Polański, Lewin-Kowalik, Kotulska, Jędrzejowska-Szypułka: "Long-term High Fat Ketogenic Diet Promotes Renal Tumor Growth in a Rat Model of Tuberous Sclerosis." in: Scientific reports, Vol. 6, pp. 21807, 2016 (PubMed). Method employed by authors: Immunohistochemistry (IHC)

Leon, Sakumi, Castillo, Sheng, Oka, Nakabeppu: "Corrigendum: 8-Oxoguanine accumulation in mitochondrial DNA causes mitochondrial dysfunction and impairs neuritogenesis in cultured adult mouse cortical neurons under oxidative conditions." in: Scientific reports, Vol. 6, pp. 24696, 2016 (PubMed). Method employed by authors: Immunohistochemistry (IHC)

Leon, Sakumi, Castillo, Sheng, Oka, Nakabeppu: "8-Oxoguanine accumulation in mitochondrial DNA causes mitochondrial dysfunction and impairs neuritogenesis in cultured adult mouse cortical neurons under oxidative conditions." in: Scientific reports, Vol. 6, pp. 22086, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Kohno, Yamamoto, Hirahashi, Kumagae, Nakamura, Oki, Oda: "Reduced MUTYH, MTH1, and OGG1 expression and TP53 mutation in diffuse-type adenocarcinoma of gastric cardia." in: Human pathology, Vol. 52, pp. 145-52, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Chaisiriwong, Wanitphakdeedecha, Sitthinamsuwan, Sampattavanich, Chatsiricharoenkul, Manuskiatti, Panich: "A Case-Control Study of Involvement of Oxidative DNA Damage and Alteration of Antioxidant Defense System in Patients with Basal Cell Carcinoma: Modulation by Tumor Removal." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 5934024, 2016 (PubMed).

Tarry-Adkins, Fernandez-Twinn, Chen, Hargreaves, Neergheen, Aiken, Ozanne: "Poor maternal nutrition and accelerated postnatal growth induces an accelerated aging phenotype and oxidative stress in skeletal muscle of male rats." in: Disease models & mechanisms, Vol. 9, Issue 10, pp. 1221-1229, 2016 (PubMed).

Yang, Chen, Chen, Kuo, Chen: "Activation of GLP-1 Receptor Enhances Neuronal Base Excision Repair via PI3K-AKT-Induced Expression of Apurinic/Apyrimidinic Endonuclease 1." in: Theranostics, Vol. 6, Issue 12, pp. 2015-2027, 2016 (PubMed).

Fridlich, Annamalai, Roy, Bernheim, Powell: "BRCA1 and BRCA2 protect against oxidative DNA damage converted into double-strand breaks during DNA replication." in: DNA repair, Vol. 30, pp. 11-20, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Zijno, De Angelis, De Berardis, Andreoli, Russo, Pietraforte, Scorza, Degan, Ponti, Rossi, Barone: "Different mechanisms are involved in oxidative DNA damage and genotoxicity induction by ZnO and TiO2 nanoparticles in human colon carcinoma cells." in: Toxicology in vitro : an international journal published in association with BIBRA, Vol. 29, Issue 7, pp. 1503-12, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Dziaman, Banaszkiewicz, Roszkowski, Gackowski, Wisniewska, Rozalski, Foksinski, Siomek, Speina, Winczura, Marszalek, Tudek, Olinski: "8-Oxo-7,8-dihydroguanine and uric acid as efficient predictors of survival in colon cancer patients." in: International journal of cancer, Vol. 134, Issue 2, pp. 376-83, 2014 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Cordero, Alcocer-Gómez, Culic, Carrión, de Miguel, Díaz-Parrado, Pérez-Villegas, Bullón, Battino, Sánchez-Alcazar: "NLRP3 inflammasome is activated in fibromyalgia: the effect of coenzyme Q10." in: Antioxidants & redox signaling, Vol. 20, Issue 8, pp. 1169-80, 2014 (PubMed).

Kubo, Morita, Nakashima, Kitao, Egashira, Saeki, Oki, Kakeji, Oda, Maehara: "Oxidative DNA damage in human esophageal cancer: clinicopathological analysis of 8-hydroxydeoxyguanosine and its repair enzyme." in: Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus, Vol. 27, Issue 3, pp. 285-93, 2014 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Xu, Wang, Guo, Zhou, Lv, Chen, Liu: "The significance of the alteration of 8-OHdG in serous ovarian carcinoma." in: Journal of ovarian research, Vol. 6, Issue 1, pp. 74, 2014 (PubMed).

Kim, Cheresh, Williams, Cheng, Ridge, Schumacker, Weitzman, Bohr, Kamp: "Mitochondria-targeted Ogg1 and aconitase-2 prevent oxidant-induced mitochondrial DNA damage in alveolar epithelial cells." in: The Journal of biological chemistry, Vol. 289, Issue 9, pp. 6165-76, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Kumar, Rao, Pal, Pal: "Hyperglycemia-induced oxidative stress induces apoptosis by inhibiting PI3-kinase/Akt and ERK1/2 MAPK mediated signaling pathway causing downregulation of 8-oxoG-DNA glycosylase levels in glial cells." in: The international journal of biochemistry & cell biology, Vol. 53, pp. 302-19, 2014 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Sagar, Kumar, Behera, Pal: "Effects of CEES and LPS synergistically stimulate oxidative stress inactivates OGG1 signaling in macrophage cells." in: Journal of hazardous materials, Vol. 278, pp. 236-49, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Wang, Wang, Chen, Zhang, Tang, Park, Cucinotta, Yu, Deng, Dynan, Doetsch, Wang: "Distinct roles of Ape1 protein, an enzyme involved in DNA repair, in high or low linear energy transfer ionizing radiation-induced cell killing." in: The Journal of biological chemistry, Vol. 289, Issue 44, pp. 30635-44, 2014 (PubMed).

Fang, Inanc, Schamus, Wang, Wei, Brown, Svilar, Sugrue, Goellner, Zeng, Yates, Lan, Vens, Sobol: "HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase β." in: Nature communications, Vol. 5, pp. 5513, 2014 (PubMed). Further details: Western Blotting