Endothelial PAS Domain Protein 1 (EPAS1) antibody (Biotin)

Antigen: Endothelial PAS Domain Protein 1 (EPAS1)

Synonyms: HLF, MOP2, ECYT4, HIF2A, PASD2, bHLHe73, EPAS1, wu:fq36f01, EPAS-1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Fish

Conjugate: Biotin

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN249868

Additional Information

Alternative name: HIF2A / HIF2 alpha

Gene ID: 2034, 13819, 29452

UniProt: P97481

Immunogen: A peptide derived from the C-terminus of mouse/human HIF-2 alpha protein.

Cross-Reactivity: Fish, Human, Mouse (Murine), Rat (Rattus)

Description: Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humansand endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functionsprimarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2gene, which is known to be selectively expressed in endothelial cells. Alternate Names: anti-Endothelial pas domain protein 1 antibody,anti-EPAS-1 antibody, anti-Hif2aantibody, anti-Hypoxia inducible factor 2 alpha subunit antibody, anti-Member of passuperfamily 2 antibody, anti-MOP2 antibody, anti-Hif-2a antibody, anti-Hif 2a antibody,anti-Hif 2 alpha antibody.

Specificity: Specific for HIF-2 alpha/EPAS. Does not cross-react with HIF-1 alpha. Species Reactivity: Mouse, rat, fish and human HIF-2 alpha. Other species have not been tested.

Application Details

Application Notes: Suggested working dilutions: immunohistochemistry - 1:100,Western Blot - 1:200-1:1000 ECL (**),**Nuclear extracts. Positive Controls: Hypoxic lysates:NB800-PC26 NB810-55229

Buffer: PBS. Preservative: no preservative.

Storage: Store at 4C. Do not freeze.

Restrictions: For Research Use only

Images

Western Blot analysis on normoxic and hypoxic nuclear rat cell lysates using ABIN151051, anti-HIF-2 alpha.

Hif-2 alpha immunoreactivity in human cardiac myocytes stained with ABIN151051.

Publications

Conrad, Freeman, Beitner-Johnson et al.: "EPAS1 trans-activation during hypoxia requires p42/p44 MAPK." in: The Journal of biological chemistry, Vol. 274, Issue 47, pp. 33709-13, 1999 (PubMed).

Bonicalzi, Groulx, de Paulsen N et al.: "Role of exon 2-encoded beta -domain of the von Hippel-Lindau tumor suppressor protein." in: The Journal of biological chemistry, Vol. 276, Issue 2, pp. 1407-16, 2001 (PubMed).

de Paulsen, Brychzy, Fournier et al.: "Role of transforming growth factor-alpha in von Hippel--Lindau (VHL)(-/-) clear cell renal carcinoma cell proliferation: a possible mechanism coupling VHL tumor suppressor inactivation and tumorigenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 4, pp. 1387-92, 2001 (PubMed).

Alvarez-Tejado, Naranjo-Suarez, Jimuenez et al.: "Hypoxia induces the activation of the phosphatidylinositol 3-kinase/Akt cell survival pathway in PC12 cells: protective role in apoptosis." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 22368-74, 2001 (PubMed).

Burke, Tang, Corke et al.: "Expression of HIF-1alpha by human macrophages: implications for the use of macrophages in hypoxia-regulated cancer gene therapy." in: The Journal of pathology, Vol. 196, Issue 2, pp. 204-12, 2002 (PubMed).

Alvarez-Tejado, Alfranca, Aragonues et al.: "Lack of evidence for the involvement of the phosphoinositide 3-kinase/Akt pathway in the activation of hypoxia-inducible factors by low oxygen tension." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13508-17, 2002 (PubMed).

Chan, Sutphin, Denko et al.: "Role of prolyl hydroxylation in oncogenically stabilized hypoxia-inducible factor-1alpha." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 40112-7, 2002 (PubMed).

Baba, Hirai, Yamada-Okabe et al.: "Loss of von Hippel-Lindau protein causes cell density dependent deregulation of CyclinD1 expression through hypoxia-inducible factor." in: Oncogene, Vol. 22, Issue 18, pp. 2728-38, 2003 (PubMed).

Corn, McDonald, Herman et al.: "Tat-binding protein-1, a component of the 26S proteasome, contributes to the E3 ubiquitin ligase function of the von Hippel-Lindau protein." in: Nature genetics, Vol. 35, Issue 3, pp. 229-37, 2003 (PubMed).

Cuevas, Hernuandez-Alcoceba, Aragones et al.: "Specific oncolytic effect of a new hypoxia-inducible factor-dependent replicative adenovirus on von Hippel-Lindau-defective renal cell carcinomas." in: Cancer research, Vol. 63, Issue 20, pp. 6877-84, 2003 (PubMed).

Biju, Neumann, Bensinger et al.: "Vhlh gene deletion induces Hif-1-mediated cell death in thymocytes." in: Molecular and cellular biology, Vol. 24, Issue 20, pp. 9038-47, 2004 (PubMed).

Asikainen, Schneider, Waleh et al.: "Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 29, pp. 10212-7, 2005 (PubMed).

Calvani, Rapisarda, Uranchimeg et al.: "Hypoxic induction of an HIF-1alpha-dependent bFGF autocrine loop drives angiogenesis in human endothelial cells." in: Blood, Vol. 107, Issue 7, pp. 2705-12, 2006 (PubMed).

Bracken, Fedele, Linke et al.: "Cell-specific regulation of hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha stabilization and transactivation in a graded oxygen environment." in: The Journal of biological chemistry, Vol. 281, Issue 32, pp. 22575-85, 2006 (PubMed).

Carroll, Kenerson, Yeung et al.: "Latent Kaposi's sarcoma-associated herpesvirus infection of endothelial cells activates hypoxia-induced factors." in: Journal of virology, Vol. 80, Issue 21, pp. 10802-12, 2006 (PubMed).

Chavez, Baranova, Lin et al.: "The transcriptional activator hypoxia inducible factor 2 (HIF-2/EPAS-1) regulates the oxygen-dependent expression of erythropoietin in cortical astrocytes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 37, pp. 9471-81, 2006 (PubMed).

Block, Gorin, Hoover et al.: "NAD(P)H oxidases regulate HIF-2alpha protein expression." in: The Journal of biological chemistry, Vol. 282, Issue 11, pp. 8019-26, 2007 (PubMed).