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Endothelial PAS Domain Protein 1 (EPAS1) (C-Term) antibody (Biotin)

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Synonyms EPAS1, ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73, HIF-2alpha, HRF, Hif2a, bhlhe73, ecyt4, hif2, hif2a, hif2alpha, hlf, mop2, pasd2, EPAS, EPAS-1
(41), (17), (16), (14), (13), (13), (9), (6), (6), (5), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Fish, Human, Mouse (Murine), Rat (Rattus)
(143), (46), (37), (1), (1), (1), (1), (1), (1), (1)
(113), (59), (3), (1)
(14), (10), (6), (6), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(143), (86), (60), (51), (45), (29), (16), (13), (11), (7), (4), (3), (2), (2), (1)
Pubmed 17 references available
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Quantity 0.1 mL
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Immunogen A peptide derived from the C-terminus of mouse/human HIF-2 alpha protein.
Specificity Specific for HIF-2 alpha/EPAS. Does not cross-react with HIF-1 alpha.
Cross-Reactivity (Details) Reactivity with sheep reported in the scientific literature (PMID: 17307811).
Purification affinity purified
Alternative Name HIF2A / HIF2 alpha (EPAS1 Antibody Abstract)
Background Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humansand endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functionsprimarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2gene, which is known to be selectively expressed in endothelial cells. Alternate Names: anti-Endothelial pas domain protein 1 antibody,anti-EPAS-1 antibody, anti-Hif2aantibody, anti-Hypoxia inducible factor 2 alpha subunit antibody, anti-Member of passuperfamily 2 antibody, anti-MOP2 antibody, anti-Hif-2a antibody, anti-Hif 2a antibody,anti-Hif 2 alpha antibody.
Gene Symbol: EPAS1
Gene ID 2034, 13819, 29452
Application Notes This HIF-2 alpha antibody is useful for Western blot, Immunoprecipitation, Chromatin Immunoprecipitation, ELISA, Gel Shift Super Assay (PMID: 15184875), Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections. In WB, this antibody recognizes a band at 118kDa representing HIF-2 alpha.
Recommended dilutions: Immunohistochemistry 1:100, Immunohistochemistry-Paraffin 1:100, Western Blot 1:200-1:1000 ECL (**)
Restrictions For Research Use only
Format Liquid
Concentration 2.0 mg/mL
Buffer PBS
Preservative Without preservative
Storage 4 °C
Storage Comment 4 °C in the dark
Supplier Images
Western Blotting (WB) image for anti-Endothelial PAS Domain Protein 1 (EPAS1) (C-Term) antibody (Biotin) (ABIN249868) Western Blot analysis on normoxic and hypoxic nuclear rat cell lysates using ABIN1510...
Immunohistochemistry (IHC) image for anti-Endothelial PAS Domain Protein 1 (EPAS1) (C-Term) antibody (Biotin) (ABIN249868) Hif-2 alpha immunoreactivity in human cardiac myocytes stained with ABIN151051.
Product cited in: Block, Gorin, Hoover et al.: "NAD(P)H oxidases regulate HIF-2alpha protein expression." in: The Journal of biological chemistry, Vol. 282, Issue 11, pp. 8019-26, 2007 (PubMed).

Chavez, Baranova, Lin et al.: "The transcriptional activator hypoxia inducible factor 2 (HIF-2/EPAS-1) regulates the oxygen-dependent expression of erythropoietin in cortical astrocytes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 37, pp. 9471-81, 2006 (PubMed).

Carroll, Kenerson, Yeung et al.: "Latent Kaposi's sarcoma-associated herpesvirus infection of endothelial cells activates hypoxia-induced factors." in: Journal of virology, Vol. 80, Issue 21, pp. 10802-12, 2006 (PubMed).

Bracken, Fedele, Linke et al.: "Cell-specific regulation of hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha stabilization and transactivation in a graded oxygen environment." in: The Journal of biological chemistry, Vol. 281, Issue 32, pp. 22575-85, 2006 (PubMed).

Calvani, Rapisarda, Uranchimeg et al.: "Hypoxic induction of an HIF-1alpha-dependent bFGF autocrine loop drives angiogenesis in human endothelial cells." in: Blood, Vol. 107, Issue 7, pp. 2705-12, 2006 (PubMed).

Asikainen, Schneider, Waleh et al.: "Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 29, pp. 10212-7, 2005 (PubMed).

Biju, Neumann, Bensinger et al.: "Vhlh gene deletion induces Hif-1-mediated cell death in thymocytes." in: Molecular and cellular biology, Vol. 24, Issue 20, pp. 9038-47, 2004 (PubMed).

Cuevas, Hernuandez-Alcoceba, Aragones et al.: "Specific oncolytic effect of a new hypoxia-inducible factor-dependent replicative adenovirus on von Hippel-Lindau-defective renal cell carcinomas." in: Cancer research, Vol. 63, Issue 20, pp. 6877-84, 2003 (PubMed).

Corn, McDonald, Herman et al.: "Tat-binding protein-1, a component of the 26S proteasome, contributes to the E3 ubiquitin ligase function of the von Hippel-Lindau protein." in: Nature genetics, Vol. 35, Issue 3, pp. 229-37, 2003 (PubMed).

Baba, Hirai, Yamada-Okabe et al.: "Loss of von Hippel-Lindau protein causes cell density dependent deregulation of CyclinD1 expression through hypoxia-inducible factor." in: Oncogene, Vol. 22, Issue 18, pp. 2728-38, 2003 (PubMed).

Chan, Sutphin, Denko et al.: "Role of prolyl hydroxylation in oncogenically stabilized hypoxia-inducible factor-1alpha." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 40112-7, 2002 (PubMed).

Alvarez-Tejado, Alfranca, Aragonues et al.: "Lack of evidence for the involvement of the phosphoinositide 3-kinase/Akt pathway in the activation of hypoxia-inducible factors by low oxygen tension." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13508-17, 2002 (PubMed).

Burke, Tang, Corke et al.: "Expression of HIF-1alpha by human macrophages: implications for the use of macrophages in hypoxia-regulated cancer gene therapy." in: The Journal of pathology, Vol. 196, Issue 2, pp. 204-12, 2002 (PubMed).

Background publications Alvarez-Tejado, Naranjo-Suarez, Jimuenez et al.: "Hypoxia induces the activation of the phosphatidylinositol 3-kinase/Akt cell survival pathway in PC12 cells: protective role in apoptosis." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 22368-74, 2001 (PubMed).

de Paulsen, Brychzy, Fournier et al.: "Role of transforming growth factor-alpha in von Hippel--Lindau (VHL)(-/-) clear cell renal carcinoma cell proliferation: a possible mechanism coupling VHL tumor suppressor inactivation and tumorigenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 4, pp. 1387-92, 2001 (PubMed).

Bonicalzi, Groulx, de Paulsen N et al.: "Role of exon 2-encoded beta -domain of the von Hippel-Lindau tumor suppressor protein." in: The Journal of biological chemistry, Vol. 276, Issue 2, pp. 1407-16, 2001 (PubMed).

Conrad, Freeman, Beitner-Johnson et al.: "EPAS1 trans-activation during hypoxia requires p42/p44 MAPK." in: The Journal of biological chemistry, Vol. 274, Issue 47, pp. 33709-13, 1999 (PubMed).

Catalog No. ABIN249868
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