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EPAS1 antibody (Endothelial PAS Domain Protein 1) (C-Term) (Biotin)

Details for Product anti-EPAS1 Antibody No. ABIN249868, Supplier: Log in to see
Antigen
  • EPAS1
  • ECYT4
  • HIF2A
  • HLF
  • MOP2
  • PASD2
  • bHLHe73
  • HIF-2alpha
  • HRF
  • Hif2a
  • bhlhe73
  • ecyt4
  • hif2
  • hif2a
  • hif2alpha
  • hlf
  • mop2
  • pasd2
  • EPAS
  • EPAS-1
  • endothelial PAS domain protein 1
  • EPAS1
  • epas1
  • Epas1
Epitope
C-Term
48
30
15
13
9
8
8
6
6
5
5
5
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Fish, Hamster, Human, Mouse (Murine), Primate, Rat (Rattus), Sheep (Ovine)
189
95
85
37
18
13
12
3
2
2
2
2
2
2
2
1
1
1
1
Host
Rabbit
125
87
3
1
Clonality
Polyclonal
Conjugate
This EPAS1 antibody is conjugated to Biotin
14
13
9
9
7
7
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
189
120
86
77
73
71
48
18
18
14
13
7
4
4
3
2
2
2
1
Options
Supplier
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Immunogen A peptide derived from the C-terminus of mouse/human HIF-2 alpha protein.
Specificity Specific for HIF-2 alpha/EPAS. Does not cross-react with HIF-1 alpha.
Purification Immunogen affinity purified
Alternative Name HIF-2 alpha/epas1 (EPAS1 Antibody Abstract)
Background Gene Symbol: EPAS1
Molecular Weight Theoretical MW: 118 kDa
Gene ID 2034
Application Notes Western Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-FrozenThis HIF-2 alpha antibody is useful for Western blot, Immunoprecipitation, Chromatin Immunoprecipitation, ELISA, Gel Shift Super Assay (PMID: 15184875), Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections. In WB, this antibody recognizes a band at 118 kDa representing HIF-2 alpha. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C in the dark.
Supplier Images
Western Blotting (WB) image for anti-EPAS1 antibody (Endothelial PAS Domain Protein 1) (C-Term) (Biotin) (ABIN249868) Western Blot analysis on normoxic and hypoxic nuclear rat cell lysates using ABIN1510...
Immunohistochemistry (IHC) image for anti-EPAS1 antibody (Endothelial PAS Domain Protein 1) (C-Term) (Biotin) (ABIN249868) Hif-2 alpha immunoreactivity in human cardiac myocytes stained with ABIN151051.
Product cited in: Block, Gorin, Hoover, Williams, Chelmicki, Clark, Yoneda, Abboud: "NAD(P)H oxidases regulate HIF-2alpha protein expression." in: The Journal of biological chemistry, Vol. 282, Issue 11, pp. 8019-26, 2007 (PubMed).

Calvani, Rapisarda, Uranchimeg, Shoemaker, Melillo: "Hypoxic induction of an HIF-1alpha-dependent bFGF autocrine loop drives angiogenesis in human endothelial cells." in: Blood, Vol. 107, Issue 7, pp. 2705-12, 2006 (PubMed).

Bracken, Fedele, Linke, Balrak, Lisy, Whitelaw, Peet: "Cell-specific regulation of hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha stabilization and transactivation in a graded oxygen environment." in: The Journal of biological chemistry, Vol. 281, Issue 32, pp. 22575-85, 2006 (PubMed).

Carroll, Kenerson, Yeung, Lagunoff: "Latent Kaposi's sarcoma-associated herpesvirus infection of endothelial cells activates hypoxia-induced factors." in: Journal of virology, Vol. 80, Issue 21, pp. 10802-12, 2006 (PubMed).

Chavez, Baranova, Lin, Pichiule: "The transcriptional activator hypoxia inducible factor 2 (HIF-2/EPAS-1) regulates the oxygen-dependent expression of erythropoietin in cortical astrocytes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 37, pp. 9471-81, 2006 (PubMed).

Asikainen, Schneider, Waleh, Clyman, Ho, Flippin, Günzler, White: "Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 29, pp. 10212-7, 2005 (PubMed).

Biju, Neumann, Bensinger, Johnson, Turka, Haase: "Vhlh gene deletion induces Hif-1-mediated cell death in thymocytes." in: Molecular and cellular biology, Vol. 24, Issue 20, pp. 9038-47, 2004 (PubMed).

Corn, McDonald, Herman, El-Deiry: "Tat-binding protein-1, a component of the 26S proteasome, contributes to the E3 ubiquitin ligase function of the von Hippel-Lindau protein." in: Nature genetics, Vol. 35, Issue 3, pp. 229-37, 2003 (PubMed).

Cuevas, Hernández-Alcoceba, Aragones, Naranjo-Suárez, Castellanos, Esteban, Martín-Puig, Landazuri, del Peso: "Specific oncolytic effect of a new hypoxia-inducible factor-dependent replicative adenovirus on von Hippel-Lindau-defective renal cell carcinomas." in: Cancer research, Vol. 63, Issue 20, pp. 6877-84, 2003 (PubMed).

Baba, Hirai, Yamada-Okabe, Hamada, Tabuchi, Kobayashi, Kondo, Yoshida, Yamashita, Kishida, Nakaigawa, Nagashima, Kubota, Yao, Ohno: "Loss of von Hippel-Lindau protein causes cell density dependent deregulation of CyclinD1 expression through hypoxia-inducible factor." in: Oncogene, Vol. 22, Issue 18, pp. 2728-38, 2003 (PubMed).

Alvarez-Tejado, Alfranca, Aragonés, Vara, Landázuri, del Peso: "Lack of evidence for the involvement of the phosphoinositide 3-kinase/Akt pathway in the activation of hypoxia-inducible factors by low oxygen tension." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13508-17, 2002 (PubMed).

Burke, Tang, Corke, Tazzyman, Ameri, Wells, Lewis: "Expression of HIF-1alpha by human macrophages: implications for the use of macrophages in hypoxia-regulated cancer gene therapy." in: The Journal of pathology, Vol. 196, Issue 2, pp. 204-12, 2002 (PubMed).

Chan, Sutphin, Denko, Giaccia: "Role of prolyl hydroxylation in oncogenically stabilized hypoxia-inducible factor-1alpha." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 40112-7, 2002 (PubMed).

Background publications Alvarez-Tejado, Naranjo-Suarez, Jiménez, Carrera, Landázuri, del Peso: "Hypoxia induces the activation of the phosphatidylinositol 3-kinase/Akt cell survival pathway in PC12 cells: protective role in apoptosis." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 22368-74, 2001 (PubMed).

Bonicalzi, Groulx, de Paulsen N, Lee: "Role of exon 2-encoded beta -domain of the von Hippel-Lindau tumor suppressor protein." in: The Journal of biological chemistry, Vol. 276, Issue 2, pp. 1407-16, 2001 (PubMed).

de Paulsen, Brychzy, Fournier, Klausner, Gnarra, Pause, Lee et al.: "Role of transforming growth factor-alpha in von Hippel--Lindau (VHL)(-/-) clear cell renal carcinoma cell proliferation: a possible mechanism coupling VHL tumor suppressor inactivation and ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 4, pp. 1387-92, 2001 (PubMed).

Conrad, Freeman, Beitner-Johnson, Millhorn: "EPAS1 trans-activation during hypoxia requires p42/p44 MAPK." in: The Journal of biological chemistry, Vol. 274, Issue 47, pp. 33709-13, 1999 (PubMed).