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ARNT antibody (Aryl Hydrocarbon Receptor Nuclear Translocator)

Details for Product anti-ARNT Antibody No. ABIN249908, Supplier: Log in to see
Antigen
  • ARNT
  • arnt1
  • Arnt1
  • bHLHe2
  • D3Ertd557e
  • Drnt
  • ESTM42
  • HIF-1-beta
  • HIF-1beta
  • hif-1beta
  • HIF1-beta
  • Hif1b
  • HIF1B
  • hif1b
  • HIF1BETA
  • hif1beta
  • mKIAA4051
  • tango
  • TANGO
  • W08714
  • xArnt
  • zgc:136664
Reactivity
Cow (Bovine), Ferret, Human, Mouse (Murine), Primate, Rat (Rattus), Sheep (Ovine)
158
65
59
29
26
26
19
3
2
2
1
1
1
Host
Mouse
75
67
22
Clonality (Clone)
Monoclonal ()
Conjugate
This ARNT antibody is un-conjugated
5
5
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
136
55
53
43
35
27
17
14
10
7
7
3
3
2
1
1
1
Supplier
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Immunogen Fusion protein containing amino acids 496-789 of human HIF-1 beta. [UniProt# P27540]
Clone H1beta234
Isotype IgG1 kappa
Purification Protein G purified
Alternative Name ARNT/HIF-1 beta (ARNT Antibody Abstract)
Background Gene Symbol: ARNT
Molecular Weight Theoretical MW: 92 kDa
Gene ID 405, 11863, 25242
UniProt P27540
Application Notes Western Blot 1:500-1:2000, Chromatin Immunoprecipitation 1:10-1:500, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin 1:100, Gel Super Shift Assays
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for HIF-1 beta Antibody Western Blot Procedure
1. Resolve aliquots (15 mg) of induced nuclear protein extracts on a SDS/6 % polyacrylamide gel.
. Transfer to nitrocellulose membranes in 20 mM Tris-HCl (pH 8.0)/150 mM glycine/20 % (vol/vol) methanol.
. Block membranes for 1.5 hours with 1X western wash buffer containing 5 % non-fat dry milk (NFDM).
. Incubate membranes for 1.5 hours at room temperature (RT) in NB 100-124 diluted 1:1,500 in 1X western wash/5 % NFDM.
. Wash with 1X western wash for 35 minutes at RT (1 X 15 minutes, 2 X 10 minutes).
. Incubate membranes with HRP conjugated anti-Mouse IgG for 1 hour (RT) in 1X western wash/5 % NFDM.
. Wash with 1X western wash for 35 minutes at RT (1 X 15 minutes, 2 X 10 minutes).
. Drain membrane and place on saran wrap.
. Using Amersham ECL Kit, mix equal volumes of two reagents. Pour over membrane (protein side facing up). Let solution sit on membrane for 15-20 seconds.
. Drain membrane and place on new saran wrap
. Wrap up membrane and expose to film.
. Develop accordingly.10X Western wash 24.2g Tris80g NaCl Tween-20 to 1 % pH 7.6 and QS to 4LStripping buffer100 mM BME2 % SDS62.5 mM Tris (pH 6.7)Incubate membrane for 30 minutes at 56 degrees C. Wash membrane 15 minutes with several changes of 1X western wash.
Restrictions For Research Use only
Format Liquid
Concentration 1.4 mg/mL
Buffer 1 X PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-ARNT antibody (Aryl Hydrocarbon Receptor Nuclear Translocator) (ABIN249908) Immunohistochemical staining of human glioblastoma multi-forme utilizing anti-HIF-1 B...
Western Blotting (WB) image for anti-ARNT antibody (Aryl Hydrocarbon Receptor Nuclear Translocator) (ABIN249908) Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [ABIN151055] - Analysis of HIF-1 b...
Product cited in: Li, Heller, Bostick, Lee, Schjerven, Kastner, Chan, Chen, Zhou: "Ikaros Inhibits Group 3 Innate Lymphoid Cell Development and Function by Suppressing the Aryl Hydrocarbon Receptor Pathway." in: Immunity, Vol. 45, Issue 1, pp. 185-97, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Thienpont, Steinbacher, Zhao, DAnna, Kuchnio, Ploumakis, Ghesquière, Van Dyck, Boeckx, Schoonjans, Hermans, Amant, Kristensen, Koh, Mazzone, Coleman, Carell, Carmeliet, Lambrechts: "Tumour hypoxia causes DNA hypermethylation by reducing TET activity." in: Nature, Vol. 537, Issue 7618, pp. 63-68, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Chromatin Immunoprecipitation

Taylor, Bagnall, Mason, Levy, Fernig, See: "Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility." in: Open biology, Vol. 6, Issue 9, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Altuntas, Rossin, Marsella, DEletto, Diaz-Hidalgo, Farrace, Campanella, Antonioli, Fimia, Piacentini: "The transglutaminase type 2 and pyruvate kinase isoenzyme M2 interplay in autophagy regulation." in: Oncotarget, Vol. 6, Issue 42, pp. 44941-54, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Luo, Chen, Chen, Alkam, Wang, Semenza: "PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia." in: Oncotarget, Vol. 7, Issue 6, pp. 6379-97, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Rossin, DEletto, Falasca, Sepe, Cocco, Fimia, Campanella, Mastroberardino, Farrace, Piacentini: "Transglutaminase 2 ablation leads to mitophagy impairment associated with a metabolic shift towards aerobic glycolysis." in: Cell death and differentiation, Vol. 22, Issue 3, pp. 408-18, 2015 (PubMed). Further details: Western Blotting

Kim, Kim, Park, Jung, Cho, Kim, Han, Choi, Kwon: "NNC 55-0396, a T-type Ca2+ channel inhibitor, inhibits angiogenesis via suppression of hypoxia-inducible factor-1α signal transduction." in: Journal of molecular medicine (Berlin, Germany), Vol. 93, Issue 5, pp. 499-509, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Xiang, Gilkes, Hu, Luo, Bullen, Liang, Semenza: "HIF-1α and TAZ serve as reciprocal co-activators in human breast cancer cells." in: Oncotarget, Vol. 6, Issue 14, pp. 11768-78, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting,Chromatin Immunoprecipitation,Immunoprecipitation

Su, Xin, Yin, Dyson, Coon, Farrow, Mestan, Ernst: "Impaired fetoplacental angiogenesis in growth-restricted fetuses with abnormal umbilical artery doppler velocimetry is mediated by aryl hydrocarbon receptor nuclear translocator (ARNT)." in: The Journal of clinical endocrinology and metabolism, Vol. 100, Issue 1, pp. E30-40, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence

Xiang, Gilkes, Hu, Takano, Luo, Lu, Bullen, Samanta, Liang, Semenza: "Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype." in: Oncotarget, Vol. 5, Issue 24, pp. 12509-27, 2015 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Frey, Stonko, Faugere, Riddle: "Hypoxia-inducible factor-1α restricts the anabolic actions of parathyroid hormone." in: Bone research, Vol. 2, pp. 14005, 2015 (PubMed). Method employed by authors: Immunofluorescence (IF)

Sena, Wang, Pawlus, Hu: "HIFs enhance the transcriptional activation and splicing of adrenomedullin." in: Molecular cancer research : MCR, Vol. 12, Issue 5, pp. 728-41, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Wang, Gilkes, Takano, Xiang, Luo, Bishop, Chaturvedi, Green, Semenza: "Hypoxia-inducible factors and RAB22A mediate formation of microvesicles that stimulate breast cancer invasion and metastasis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 31, pp. E3234-42, 2014 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Peng, Yuan, Khan, Nanduri, Makarenko, Reddy, Vasavda, Kumar, Semenza, Prabhakar: "Regulation of hypoxia-inducible factor-α isoforms and redox state by carotid body neural activity in rats." in: The Journal of physiology, Vol. 592, Issue 17, pp. 3841-58, 2014 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Pawlus, Wang, Hu: "STAT3 and HIF1α cooperatively activate HIF1 target genes in MDA-MB-231 and RCC4 cells." in: Oncogene, Vol. 33, Issue 13, pp. 1670-9, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Xiang, Gilkes, Chaturvedi, Luo, Hu, Takano, Liang, Semenza: "Ganetespib blocks HIF-1 activity and inhibits tumor growth, vascularization, stem cell maintenance, invasion, and metastasis in orthotopic mouse models of triple-negative breast cancer." in: Journal of molecular medicine (Berlin, Germany), Vol. 92, Issue 2, pp. 151-64, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Gilkes, Xiang, Lee, Chaturvedi, Hubbi, Wirtz, Semenza: "Hypoxia-inducible factors mediate coordinated RhoA-ROCK1 expression and signaling in breast cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 3, pp. E384-93, 2014 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Takano, Peng, Kumar, Luo, Hu, Shimoda, Suematsu, Prabhakar, Semenza: "Hypoxia-inducible factors regulate human and rat cystathionine ?-synthase gene expression." in: The Biochemical journal, Vol. 458, Issue 2, pp. 203-11, 2014 (PubMed). Further details: Chromatin Immunoprecipitation

Zhang, Ma: "Nerve growth factor regulates the expression of vascular endothelial growth factor in human HaCaT keratinocytes via PI3K/mTOR pathway." in: Genetics and molecular research : GMR, Vol. 13, Issue 4, pp. 9324-35, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Chaturvedi, Gilkes, Takano, Semenza: "Hypoxia-inducible factor-dependent signaling between triple-negative breast cancer cells and mesenchymal stem cells promotes macrophage recruitment." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 20, pp. E2120-9, 2014 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation