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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) antibody
|Synonyms||HIF1B, TANGO, bHLHe2, HIF1BETA, HIF-1beta, Drnt, Hif1b, ESTM42, W08714, KIAA4051, mKIAA4051, D3Ertd557e, Arnt1, ARNT, MGC136664, zgc:136664, arnt1, hif1b, tango, xArnt, hif1beta, hif-1beta, AHA-1|
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|12 references available|
|Quantity||25 µl (1.4 mg/ml) (Variants)|
|Price||Product not available in this region.|
|Alternative name||HIF1 beta / ARNT|
|Immunogen||Fusion protein containing amino acids 496-789 of human HIF-1 beta.|
|Cross-Reactivity||Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)|
|Description||Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease, and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as aposttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composedof HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated.However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 orPro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degradedin proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIFdegradation and increases transcriptional activity. Therefore, the concentration of HIF-1alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition.HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) geneproducts. Alternate Names: anti-ARNT antibody, anti-Aryl Hydrocarbon Receptor Nuclear Translocator antibody,anti-Dioxin Receptor antibody, anti-Hypoxia Inducible Factor 1 antibody, anti-Tangoantibody, anti-HIF1 beta antibody, anti-HIF1beta antibody.|
|Specificity||This antibody is specific for HIF 1 beta /ARNT (1). Species Reactivity: Human, bovine, sheep, mouse, rat and ferret. Other species have not been tested.|
|Application Notes||Suggested working dilutions: Western Blot 1:500,Immunohistochemistry-Paraffin 1:100|
|Buffer||PBS. Preservative: 0.05% sodium azide.|
|Storage||Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.|
|Research Area||Transcription Factors|
|Restrictions||For Research Use only|
|Immunohistochemistry: HIF-1 Beta Antibody (H1beta234) [ABIN151055SS] - Immunohistochemical staining of human glioblastoma multi-forme utilizing anti-HIF-1 Beta (ABIN151055).|
Bernaudin, Tang, Reilly et al.: "Brain genomic response following hypoxia and re-oxygenation in the neonatal rat. Identification of genes that might contribute to hypoxia-induced ischemic tolerance." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39728-38, 2002 (PubMed).
Elvert, Kappel, Heidenreich et al.: "Cooperative interaction of hypoxia-inducible factor-2alpha (HIF-2alpha ) and Ets-1 in the transcriptional activation of vascular endothelial growth factor receptor-2 (Flk-1)." in: The Journal of biological chemistry, Vol. 278, Issue 9, pp. 7520-30, 2003 (PubMed).
Mabjeesh, Willard, Harris et al.: "Dibenzoylmethane, a natural dietary compound, induces HIF-1 alpha and increases expression of VEGF." in: Biochemical and biophysical research communications, Vol. 303, Issue 1, pp. 279-86, 2003 (PubMed).
Mabjeesh, Escuin, LaVallee et al.: "2ME2 inhibits tumor growth and angiogenesis by disrupting microtubules and dysregulating HIF." in: Cancer cell, Vol. 3, Issue 4, pp. 363-75, 2003 (PubMed).
Kasuno, Takabuchi, Fukuda et al.: "Nitric oxide induces hypoxia-inducible factor 1 activation that is dependent on MAPK and phosphatidylinositol 3-kinase signaling." in: The Journal of biological chemistry, Vol. 279, Issue 4, pp. 2550-8, 2004 (PubMed).
Alam, Maizels, Park et al.: "Follicle-stimulating hormone activation of hypoxia-inducible factor-1 by the phosphatidylinositol 3-kinase/AKT/Ras homolog enriched in brain (Rheb)/mammalian target of rapamycin (mTOR) pathway is necessary for induction of select protein markers of follic" in: The Journal of biological chemistry, Vol. 279, Issue 19, pp. 19431-40, 2004 (PubMed).
Arsham, Plas, Thompson et al.: "Akt and hypoxia-inducible factor-1 independently enhance tumor growth and angiogenesis." in: Cancer research, Vol. 64, Issue 10, pp. 3500-7, 2004 (PubMed).
Hirota, Fukuda, Takabuchi et al.: "Induction of hypoxia-inducible factor 1 activity by muscarinic acetylcholine receptor signaling." in: The Journal of biological chemistry, Vol. 279, Issue 40, pp. 41521-8, 2004 (PubMed).
Amir, Wang, Matzkin et al.: "MSF-A interacts with hypoxia-inducible factor-1alpha and augments hypoxia-inducible factor transcriptional activation to affect tumorigenicity and angiogenesis." in: Cancer research, Vol. 66, Issue 2, pp. 856-66, 2006 (PubMed).
Hui, Bauer, Striet et al.: "Calcium signaling stimulates translation of HIF-alpha during hypoxia." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 3, pp. 466-75, 2006 (PubMed).
Nakamura, Martin, Jackson et al.: "Brain-derived neurotrophic factor activation of TrkB induces vascular endothelial growth factor expression via hypoxia-inducible factor-1alpha in neuroblastoma cells." in: Cancer research, Vol. 66, Issue 8, pp. 4249-55, 2006 (PubMed).
Asikainen, Chang, Coalson et al.: "Improved lung growth and function through hypoxia-inducible factor in primate chronic lung disease of prematurity." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 10, pp. 1698-700, 2006 (PubMed).
|Hosts||Rabbit (39), Mouse (14), Goat (1)|
|Reactivities||Human (51), Mouse (Murine) (29), Rat (Rattus) (24), Cow (Bovine) (21), Chicken (19), Dog (Canine) (19), Ferret (2), Sheep (Ovine) (2), Cat (Feline) (1), Primate (1)|
|Applications||Western Blotting (WB) (37), Immunofluorescence (IF) (27), ELISA (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunoprecipitation (IP) (7), Immunohistochemistry (IHC) (5), Immunocytochemistry (ICC) (4), Flow Cytometry (FACS) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (DB) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||Transcript Variant 1, N-Term (5), N-Term (4), AA 771-789 (2), C-Term (2), AA 399-777 (1), AA 517-691 (1), AA 528-544 (1), Center, Val528 (1)|