Clock Homolog (Mouse) (CLOCK) antibody

Antigen: Clock Homolog (Mouse) (CLOCK)

Synonyms: KAT13D, bHLHe8, KIAA0334, mKIAA0334, 5330400M04Rik, clk, Xclk, XClock, CLOCK, DKFZp469L011, zCLOCK1, zfCLOCK1, CLK, Jerk, Jrk, PAS1, dCLK, dCLK/JRK, dCLOCK, dClck, DmelCG7391, CG7391

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN249944

Additional Information

Alternative name: CLOCK

Gene ID: 12753

Immunogen: Two peptides derived from mouse CLOCK, conjugated to albumin.

Cross-Reactivity: Human

Format: Serum

Description: CLOCK, a basic helix-loop-helix (bHLH)/PAS protein, is an important factor in themolecular clock mechanism. Mutations in this protein lead to abnormal circadianbehavior and defective transcriptional activity. CLOCK has been shown to interactstrongly with other bHLH-PAS proteins such as MOP3, a BMAL1 isoform. Alternate Names: anti-KIAA0334 antibody.

Specificity: This antibody is specific for mCLOCK. Species Reactivity: Peptide 1 shows significant homology to human and Gallus gallus. Peptide 2 shows82% homology to Gallus gallus.

Application Details

Application Notes: Western blotting (suggested working dilution 1:1,000). Antisera was tested againstbacterially expressed fragment of CLOCK expressed in BL21 cells or the full lengthprotein expressed in an in vitro translation system (reticulocyte lysate).Also works for ChIP Assay (request specific protocol if needed).

Purification: Serum

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Research Area: Cardiovascular, Atherosclerosis, Transcription Factors, Metabolism

Restrictions: For Research Use only

Publications

Hogenesch, Gu, Jain et al.: "The basic-helix-loop-helix-PAS orphan MOP3 forms transcriptionally active complexes with circadian and hypoxia factors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 10, pp. 5474-9, 1998 (PubMed).

Jin, Shearman, Weaver et al.: "A molecular mechanism regulating rhythmic output from the suprachiasmatic circadian clock." in: Cell, Vol. 96, Issue 1, pp. 57-68, 1999 (PubMed).

Shearman, Weaver: "Photic induction of Period gene expression is reduced in Clock mutant mice." in: Neuroreport, Vol. 10, Issue 3, pp. 613-8, 1999 (PubMed).

Muunoz, Brewer, Baler: "Circadian Transcription. Thinking outside the E-Box." in: The Journal of biological chemistry, Vol. 277, Issue 39, pp. 36009-17, 2002 (PubMed).