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Clock Homolog (Mouse) (CLOCK) antibody

Details for Product No. ABIN249944, Supplier: Login to see
Antigen
Reactivity
Human, Mouse (Murine)
94
36
23
3
3
2
2
1
1
Host
Rabbit
62
34
10
1
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
91
45
29
16
7
6
6
4
3
2
1
1
1
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Immunogen Two peptides derived from mouse CLOCK conjugated to albumin.
Purification Whole antisera
Alternative Name CLOCK (CLOCK Antibody Abstract)
Background CLOCK, a basic helix-loop-helix (bHLH)/PAS protein, is an important factor in themolecular clock mechanism. Mutations in this protein lead to abnormal circadianbehavior and defective transcriptional activity. CLOCK has been shown to interactstrongly with other bHLH-PAS proteins such as MOP3, a BMAL1 isoform. Alternate Names: anti-KIAA0334 antibody, anti-KAT13D antibody, anti-ChIp Grade antibody, anti KAT13D/ CLOCK antibody.
Gene Symbol: CLOCK
Gene ID 9575
UniProt O08785
Research Area Cardiovascular, Atherosclerosis, Transcription Factors, Metabolism
Pathways
Application Notes This CLOCK antibody is useful for Western blot and Chromatin Immunoprecipitation. It has been tested against bacterially expressed fragment of CLOCK expressed in BL21 cells or the full length protein expressed in an in vitro translation system (reticulocyte lysate).
Recommended dilutions: Chromatin Immunoprecipitation 1:10-1:500, Western Blot 1:1000
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
Western Blotting (WB) image for anti-Clock Homolog (Mouse) (CLOCK) antibody (ABIN249944) Lanes 3-5 contained alpha CLOCK immunoprecipitated DNA (15, 10 and 5 ul of antisera a...
Product cited in: Muñoz, Brewer, Baler: "Circadian Transcription. Thinking outside the E-Box." in: The Journal of biological chemistry, Vol. 277, Issue 39, pp. 36009-17, 2002 (PubMed).

Background publications Shearman, Weaver: "Photic induction of Period gene expression is reduced in Clock mutant mice." in: Neuroreport, Vol. 10, Issue 3, pp. 613-8, 1999 (PubMed).

Jin, Shearman, Weaver et al.: "A molecular mechanism regulating rhythmic output from the suprachiasmatic circadian clock." in: Cell, Vol. 96, Issue 1, pp. 57-68, 1999 (PubMed).

Hogenesch, Gu, Jain et al.: "The basic-helix-loop-helix-PAS orphan MOP3 forms transcriptionally active complexes with circadian and hypoxia factors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 10, pp. 5474-9, 1998 (PubMed).