Endothelial PAS Domain Protein 1 (EPAS1) (AA 535-631) antibody (Biotin)

Details for Product No. ABIN250055
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Antigen
Synonyms EPAS1, ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73, HIF-2alpha, HRF, Hif2a, bhlhe73, ecyt4, hif2, hif2a, hif2alpha, hlf, mop2, pasd2, EPAS, EPAS-1
Epitope
AA 535-631
(32), (14), (7), (6), (6), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(104), (41), (30), (13), (13), (7), (1), (1), (1), (1)
Host
Mouse
(78), (47), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
(10), (5), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(111), (54), (42), (38), (36), (27), (16), (15), (10), (6), (2), (1), (1)
Pubmed 15 references available
Quantity 0.1 mL
Shipping to United States (Change)
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Catalog No. ABIN250055
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Immunogen Human HIF-2 alpha, corresponding to amino acids 535-631.
Clone Ep190b
Isotype IgG1
Specificity This is specific for HIF-2 alpha and does not cross-react with HIF-1 alpha.
Cross-Reactivity (Details) Ability to use in mouse is mixed with some positive,some negative results.
Purification Protein G purified
Alternative Name HIF2A / HIF2 alpha
Background Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humansand endothelial cells of the embryonic and adult mouse, whereas HIF-1 alpha functionsprimarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2gene, which is known to be selectively expressed in endothelial cells. Alternate Names: anti-Endothelial pas domain protein 1 antibody, anti-Hif2a antibody, anti-Hypoxiainducible factor 2 alpha subunit antibody, anti-Member of pas superfamily 2 antibody,anti-MOP2 antibody, anti-EPAS1 antibody, anti-Hif-2a antibody, anti-Hif 2a antibody,anti-Hif 2 alpha antibody.
Gene Symbol: EPAS1
Gene ID 2034, 13819, 29452
UniProt Q99814
Application Notes This HIF-2 alpha (ep190b) antibody is useful for ELISA, Flow Cytometry, Immunohistochemistry on paraffin-embedded sections and Western Blot. In WB, it recognizes a band at ~118 kDa representing HIF-2 alpha.
Recommended dilutions: Immunohistochemistry 1:150-1:300, Immunohistochemistry-Paraffin 1:150-1:300, Western Blot 1:500
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Preservative Without preservative
Storage 4 °C
Storage Comment 4 °C in the dark
Supplier Images
anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 535-631) antibody (Biotin) anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 535-631) antibody (Biotin)
anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 535-631) antibody (Biotin) (2) Staining of heart, cardiac myocytes using ABIN151063.
General Wiesener, Turley, Allen et al.: "Induction of endothelial PAS domain protein-1 by hypoxia: characterization and comparison with hypoxia-inducible factor-1alpha." in: Blood, Vol. 92, Issue 7, pp. 2260-8, 1998 (PubMed).

Xia, Kageyama, Hayashi et al.: "Positive expression of HIF-2alpha/EPAS1 in invasive bladder cancer." in: Urology, Vol. 59, Issue 5, pp. 774-8, 2002 (PubMed).

Mohan, Burk: "von Hippel-Lindau protein complex is regulated by cell density." in: Oncogene, Vol. 22, Issue 34, pp. 5270-80, 2003 (PubMed).

Zimmer, Doucette, Siddiqui et al.: "Inhibition of hypoxia-inducible factor is sufficient for growth suppression of VHL-/- tumors." in: Molecular cancer research : MCR, Vol. 2, Issue 2, pp. 89-95, 2004 (PubMed).

Pan, Oprysko, Asham et al.: "p53 cannot be induced by hypoxia alone but responds to the hypoxic microenvironment." in: Oncogene, Vol. 23, Issue 29, pp. 4975-83, 2004 (PubMed).

Koike, Kimura, Miyazaki et al.: "Hypoxia induces adhesion molecules on cancer cells: A missing link between Warburg effect and induction of selectin-ligand carbohydrates." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 21, pp. 8132-7, 2004 (PubMed).

Harvey, Kind, Pantaleon et al.: "Oxygen-regulated gene expression in bovine blastocysts." in: Biology of reproduction, Vol. 71, Issue 4, pp. 1108-19, 2004 (PubMed).

Mekhail, Gunaratnam, Bonicalzi et al.: "HIF activation by pH-dependent nucleolar sequestration of VHL." in: Nature cell biology, Vol. 6, Issue 7, pp. 642-7, 2004 (PubMed).

Grabmaier, A de Weijert, Verhaegh et al.: "Strict regulation of CAIX(G250/MN) by HIF-1alpha in clear cell renal cell carcinoma." in: Oncogene, Vol. 23, Issue 33, pp. 5624-31, 2004 (PubMed).

Rathmell, Hickey, Bezman et al.: "In vitro and in vivo models analyzing von Hippel-Lindau disease-specific mutations." in: Cancer research, Vol. 64, Issue 23, pp. 8595-603, 2004 (PubMed).

Yoshimura, Dhar, Kohno et al.: "Prognostic impact of hypoxia-inducible factors 1alpha and 2alpha in colorectal cancer patients: correlation with tumor angiogenesis and cyclooxygenase-2 expression." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 24, pp. 8554-60, 2004 (PubMed).

Nakamura, Abreu-e-Lima, Awakura et al.: "Clusterin is a secreted marker for a hypoxia-inducible factor-independent function of the von Hippel-Lindau tumor suppressor protein." in: The American journal of pathology, Vol. 168, Issue 2, pp. 574-84, 2006 (PubMed).

Zheng, Ruas, Cao et al.: "Cell-type-specific regulation of degradation of hypoxia-inducible factor 1 alpha: role of subcellular compartmentalization." in: Molecular and cellular biology, Vol. 26, Issue 12, pp. 4628-41, 2006 (PubMed).

Koizume, Jin, Miyagi et al.: "Activation of cancer cell migration and invasion by ectopic synthesis of coagulation factor VII." in: Cancer research, Vol. 66, Issue 19, pp. 9453-60, 2006 (PubMed).

Bernhardt, Wiesener, Weidemann et al.: "Involvement of hypoxia-inducible transcription factors in polycystic kidney disease." in: The American journal of pathology, Vol. 170, Issue 3, pp. 830-42, 2007 (PubMed).

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