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EPAS1 antibody (Endothelial PAS Domain Protein 1) (AA 535-631) (HRP)

Details for Product anti-EPAS1 Antibody No. ABIN250056, Supplier: Log in to see
Antigen
  • bHLHe73
  • bhlhe73
  • ECYT4
  • ecyt4
  • EPAS
  • EPAS-1
  • EPAS1
  • HIF-2alpha
  • hif2
  • hif2a
  • Hif2a
  • HIF2A
  • hif2alpha
  • HLF
  • hlf
  • HRF
  • MOP2
  • mop2
  • PASD2
  • pasd2
Epitope
AA 535-631
47
22
17
14
13
13
8
5
5
5
5
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
153
56
48
6
6
1
1
1
1
1
1
Host
Mouse
109
68
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
This EPAS1 antibody is conjugated to HRP
13
11
7
7
7
6
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
150
88
62
49
46
30
20
11
9
8
4
4
3
2
1
1
Supplier
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Immunogen Human HIF-2 alpha, corresponding to amino acids 535-631.
Clone Ep190b
Isotype IgG1
Specificity This is specific for HIF-2 alpha and does not cross-react with HIF-1 alpha.
Cross-Reactivity (Details) Ability to use in mouse is mixed with some positive,some negative results.
Purification Protein G purified
Alternative Name HIF-2 alpha/epas1 (EPAS1 Antibody Abstract)
Background Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humansand endothelial cells of the embryonic and adult mouse, whereas HIF-1 alpha functionsprimarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2gene, which is known to be selectively expressed in endothelial cells. Alternate Names: anti-Endothelial pas domain protein 1 antibody, anti-Hif2a antibody, anti-Hypoxiainducible factor 2 alpha subunit antibody, anti-Member of pas superfamily 2 antibody,anti-MOP2 antibody, anti-EPAS1 antibody, anti-Hif-2a antibody, anti-Hif 2a antibody,anti-Hif 2 alpha antibody.
Gene Symbol: EPAS1
Gene ID 2034, 13819, 29452
UniProt Q99814
Application Notes This HIF-2 alpha (ep190b) antibody is useful for ELISA, Flow Cytometry, Immunohistochemistry on paraffin-embedded sections and Western Blot. In WB, it recognizes a band at ~118 kDa representing HIF-2 alpha.
Recommended dilutions: Immunohistochemistry 1.0 µg, Immunohistochemistry-Paraffin 1:150-1:300, Western Blot 1:500
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Preservative Without preservative
Storage 4 °C
Storage Comment 4 °C in the dark
Supplier Images
Western Blotting (WB) image for anti-EPAS1 antibody (Endothelial PAS Domain Protein 1) (AA 535-631) (HRP) (ABIN250056) anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 535-631) antibody (HRP)
Immunohistochemistry (IHC) image for anti-EPAS1 antibody (Endothelial PAS Domain Protein 1) (AA 535-631) (HRP) (ABIN250056) Staining of heart, cardiac myocytes using ABIN151063.
Product cited in: Bernhardt, Wiesener, Weidemann, Schmitt, Weichert, Lechler, Campean, Ong, Willam, Gretz, Eckardt: "Involvement of hypoxia-inducible transcription factors in polycystic kidney disease." in: The American journal of pathology, Vol. 170, Issue 3, pp. 830-42, 2007

Nakamura, Abreu-e-Lima, Awakura, Inoue, Kamoto, Ogawa, Kotani, Manabe, Zhang, Kondo, Nosue, Kaelin: "Clusterin is a secreted marker for a hypoxia-inducible factor-independent function of the von Hippel-Lindau tumor suppressor protein." in: The American journal of pathology, Vol. 168, Issue 2, pp. 574-84, 2006

Zheng, Ruas, Cao, Salomons, Cao, Poellinger, Pereira: "Cell-type-specific regulation of degradation of hypoxia-inducible factor 1 alpha: role of subcellular compartmentalization." in: Molecular and cellular biology, Vol. 26, Issue 12, pp. 4628-41, 2006

Koizume, Jin, Miyagi, Hirahara, Nakamura, Piao, Asai, Yoshida, Tsuchiya, Ruf, Miyagi: "Activation of cancer cell migration and invasion by ectopic synthesis of coagulation factor VII." in: Cancer research, Vol. 66, Issue 19, pp. 9453-60, 2006

Harvey, Kind, Pantaleon, Armstrong, Thompson: "Oxygen-regulated gene expression in bovine blastocysts." in: Biology of reproduction, Vol. 71, Issue 4, pp. 1108-19, 2004

Mekhail, Gunaratnam, Bonicalzi, Lee: "HIF activation by pH-dependent nucleolar sequestration of VHL." in: Nature cell biology, Vol. 6, Issue 7, pp. 642-7, 2004

Zimmer, Doucette, Siddiqui, Iliopoulos: "Inhibition of hypoxia-inducible factor is sufficient for growth suppression of VHL-/- tumors." in: Molecular cancer research : MCR, Vol. 2, Issue 2, pp. 89-95, 2004

Pan, Oprysko, Asham, Koch, Simon: "p53 cannot be induced by hypoxia alone but responds to the hypoxic microenvironment." in: Oncogene, Vol. 23, Issue 29, pp. 4975-83, 2004

Koike, Kimura, Miyazaki, Yabuta, Kumamoto, Takenoshita, Chen, Kobayashi, Hosokawa, Taniguchi, Kojima, Ishida, Kawakita, Yamamoto, Takematsu, Suzuki, Kozutsumi, Kannagi, Kanangi: "Hypoxia induces adhesion molecules on cancer cells: A missing link between Warburg effect and induction of selectin-ligand carbohydrates." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 21, pp. 8132-7, 2004

Grabmaier, A de Weijert, Verhaegh, Schalken, Oosterwijk: "Strict regulation of CAIX(G250/MN) by HIF-1alpha in clear cell renal cell carcinoma." in: Oncogene, Vol. 23, Issue 33, pp. 5624-31, 2004

Rathmell, Hickey, Bezman, Chmielecki, Carraway, Simon: "In vitro and in vivo models analyzing von Hippel-Lindau disease-specific mutations." in: Cancer research, Vol. 64, Issue 23, pp. 8595-603, 2004

Yoshimura, Dhar, Kohno, Kubota, Fujii, Ueda, Kinugasa, Tachibana, Nagasue: "Prognostic impact of hypoxia-inducible factors 1alpha and 2alpha in colorectal cancer patients: correlation with tumor angiogenesis and cyclooxygenase-2 expression." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 24, pp. 8554-60, 2004

Mohan, Burk: "von Hippel-Lindau protein complex is regulated by cell density." in: Oncogene, Vol. 22, Issue 34, pp. 5270-80, 2003

Xia, Kageyama, Hayashi, Hyochi, Kawakami, Kihara: "Positive expression of HIF-2alpha/EPAS1 in invasive bladder cancer." in: Urology, Vol. 59, Issue 5, pp. 774-8, 2002

Background publications Wiesener, Turley, Allen, Willam, Eckardt, Talks, Wood, Gatter, Harris, Pugh, Ratcliffe, Maxwell: "Induction of endothelial PAS domain protein-1 by hypoxia: characterization and comparison with hypoxia-inducible factor-1alpha." in: Blood, Vol. 92, Issue 7, pp. 2260-8, 1998