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EPAS1 antibody (Endothelial PAS Domain Protein 1) (HRP)

Details for Product anti-EPAS1 Antibody No. ABIN250056, Supplier: Log in to see
Antigen
  • EPAS1
  • ECYT4
  • HIF2A
  • HLF
  • MOP2
  • PASD2
  • bHLHe73
  • HIF-2alpha
  • HRF
  • Hif2a
  • bhlhe73
  • ecyt4
  • hif2
  • hif2a
  • hif2alpha
  • hlf
  • mop2
  • pasd2
  • EPAS
  • EPAS-1
  • endothelial PAS domain protein 1
  • EPAS1
  • epas1
  • Epas1
Reactivity
Hamster, Human, Mouse (Murine), Rat (Rattus)
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87
80
36
19
13
13
1
1
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1
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1
Host
Mouse
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86
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
This EPAS1 antibody is conjugated to HRP
15
13
9
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7
4
4
4
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3
3
2
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2
1
1
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1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
176
116
78
75
72
58
47
21
11
11
7
4
3
3
2
2
1
Options
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Immunogen Human HIF-2 alpha, corresponding to amino acids 535-631. [UniProt# Q99814]
Clone Ep190b
Isotype IgG1
Specificity This is specific for HIF-2 alpha and does not cross-react with HIF-1 alpha.
Cross-Reactivity (Details) Ability to use in mouse is mixed with some positive,some negative results.
Purification Protein G purified
Alternative Name HIF-2 alpha/epas1 (EPAS1 Antibody Abstract)
Background Gene Symbol: EPAS1
Molecular Weight Theoretical MW: 118 kDa
Gene ID 2034
UniProt Q99814
Application Notes Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry-ParaffinThis HIF-2 alpha (ep190b) antibody is useful for ELISA, Flow Cytometry, Immunohistochemistry on paraffin-embedded sections and Western Blot. In WB, it recognizes a band at ~118 kDa representing HIF-2 alpha. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Buffer PBS
Buffer contains: No Preservative
Preservative Without preservative
Storage 4 °C
Storage Comment Store at 4°C in the dark.
Supplier Images
Western Blotting (WB) image for anti-EPAS1 antibody (Endothelial PAS Domain Protein 1)  (HRP) (ABIN250056) anti-Endothelial PAS Domain Protein 1 (EPAS1) antibody (HRP)
Immunohistochemistry (IHC) image for anti-EPAS1 antibody (Endothelial PAS Domain Protein 1)  (HRP) (ABIN250056) Staining of heart, cardiac myocytes using ABIN151063.
Product cited in: Szendrői, Szász, Kardos, Tőkés, Idan, Szűcs, Kulka, Nyirády, Szendrői, Szállási, Győrffy, Tímár: "Opposite prognostic roles of HIF1α and HIF2α expressions in bone metastatic clear cell renal cell cancer." in: Oncotarget, Vol. 7, Issue 27, pp. 42086-42098, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Nüsken, Wohlfarth, Lippach, Rauh, Schneider, Dötsch, Nüsken: "Reduced Perinatal Leptin Availability May Contribute to Adverse Metabolic Programming in a Rat Model of Uteroplacental Insufficiency." in: Endocrinology, Vol. 157, Issue 5, pp. 1813-25, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Adamski, Price, Dive, Makin: "Hypoxia-induced cytotoxic drug resistance in osteosarcoma is independent of HIF-1Alpha." in: PLoS ONE, Vol. 8, Issue 6, pp. e65304, 2013 (PubMed). (Sample species: Human).

Xie, Collins: "Transcriptional regulation of the Menkes copper ATPase (Atp7a) gene by hypoxia-inducible factor (HIF2{alpha}) in intestinal epithelial cells." in: American journal of physiology. Cell physiology, Vol. 300, Issue 6, pp. C1298-305, 2011 (PubMed).

Bernhardt, Wiesener, Weidemann, Schmitt, Weichert, Lechler, Campean, Ong, Willam, Gretz, Eckardt: "Involvement of hypoxia-inducible transcription factors in polycystic kidney disease." in: The American journal of pathology, Vol. 170, Issue 3, pp. 830-42, 2007 (PubMed).

Nakamura, Abreu-e-Lima, Awakura, Inoue, Kamoto, Ogawa, Kotani, Manabe, Zhang, Kondo, Nosé, Kaelin: "Clusterin is a secreted marker for a hypoxia-inducible factor-independent function of the von Hippel-Lindau tumor suppressor protein." in: The American journal of pathology, Vol. 168, Issue 2, pp. 574-84, 2006 (PubMed).

Zheng, Ruas, Cao, Salomons, Cao, Poellinger, Pereira: "Cell-type-specific regulation of degradation of hypoxia-inducible factor 1 alpha: role of subcellular compartmentalization." in: Molecular and cellular biology, Vol. 26, Issue 12, pp. 4628-41, 2006 (PubMed).

Koizume, Jin, Miyagi, Hirahara, Nakamura, Piao, Asai, Yoshida, Tsuchiya, Ruf, Miyagi: "Activation of cancer cell migration and invasion by ectopic synthesis of coagulation factor VII." in: Cancer research, Vol. 66, Issue 19, pp. 9453-60, 2006 (PubMed).

Harvey, Kind, Pantaleon, Armstrong, Thompson: "Oxygen-regulated gene expression in bovine blastocysts." in: Biology of reproduction, Vol. 71, Issue 4, pp. 1108-19, 2004 (PubMed).

Mekhail, Gunaratnam, Bonicalzi, Lee: "HIF activation by pH-dependent nucleolar sequestration of VHL." in: Nature cell biology, Vol. 6, Issue 7, pp. 642-7, 2004 (PubMed).

Zimmer, Doucette, Siddiqui, Iliopoulos: "Inhibition of hypoxia-inducible factor is sufficient for growth suppression of VHL-/- tumors." in: Molecular cancer research : MCR, Vol. 2, Issue 2, pp. 89-95, 2004 (PubMed).

Pan, Oprysko, Asham, Koch, Simon: "p53 cannot be induced by hypoxia alone but responds to the hypoxic microenvironment." in: Oncogene, Vol. 23, Issue 29, pp. 4975-83, 2004 (PubMed).

Koike, Kimura, Miyazaki, Yabuta, Kumamoto, Takenoshita, Chen, Kobayashi, Hosokawa, Taniguchi, Kojima, Ishida, Kawakita, Yamamoto, Takematsu, Suzuki, Kozutsumi, Kannagi, Kanangi: "Hypoxia induces adhesion molecules on cancer cells: A missing link between Warburg effect and induction of selectin-ligand carbohydrates." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 21, pp. 8132-7, 2004 (PubMed).

Grabmaier, A de Weijert, Verhaegh, Schalken, Oosterwijk: "Strict regulation of CAIX(G250/MN) by HIF-1alpha in clear cell renal cell carcinoma." in: Oncogene, Vol. 23, Issue 33, pp. 5624-31, 2004 (PubMed).

Rathmell, Hickey, Bezman, Chmielecki, Carraway, Simon: "In vitro and in vivo models analyzing von Hippel-Lindau disease-specific mutations." in: Cancer research, Vol. 64, Issue 23, pp. 8595-603, 2004 (PubMed).

Yoshimura, Dhar, Kohno, Kubota, Fujii, Ueda, Kinugasa, Tachibana, Nagasue: "Prognostic impact of hypoxia-inducible factors 1alpha and 2alpha in colorectal cancer patients: correlation with tumor angiogenesis and cyclooxygenase-2 expression." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 24, pp. 8554-60, 2004 (PubMed).

Mohan, Burk: "von Hippel-Lindau protein complex is regulated by cell density." in: Oncogene, Vol. 22, Issue 34, pp. 5270-80, 2003 (PubMed).

Xia, Kageyama, Hayashi, Hyochi, Kawakami, Kihara: "Positive expression of HIF-2alpha/EPAS1 in invasive bladder cancer." in: Urology, Vol. 59, Issue 5, pp. 774-8, 2002 (PubMed).

Background publications Wiesener, Turley, Allen, Willam, Eckardt, Talks, Wood, Gatter, Harris, Pugh, Ratcliffe, Maxwell: "Induction of endothelial PAS domain protein-1 by hypoxia: characterization and comparison with hypoxia-inducible factor-1alpha." in: Blood, Vol. 92, Issue 7, pp. 2260-8, 1998 (PubMed).