Images for product: anti-Aralkylamine N-Acetyltransferase (AANAT) antibody
AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde, 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h.
Western blot analysis of hAANAT. Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity (~3.5 nmol of tryptamine acetylated per hour) in 65 ug and 2.6 ng of protein, respectively. A sample of a homogenized human pineal gland containing 33.5 ug of protein was loaded on a separate gel.