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Aralkylamine N-Acetyltransferase (AANAT) antibody

Details for Product No. ABIN250114, Supplier: Login to see
Antigen
  • DSPS
  • SNAT
  • AA-NAT
  • Nat4
  • Nat-2
  • Snat
Reactivity
Human
41
22
19
Host
Rabbit
44
Clonality
Polyclonal
Conjugate
Un-conjugated
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
29
16
13
4
4
1
1
1
Supplier
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Immunogen Human Serotonin N-acetyltransferase
Specificity This is specific for primate AANAT (3,4).
No Cross-Reactivity Cow (Bovine), Chicken, Rat (Rattus), Zebrafish
Purification affinity purified
Alternative Name Serotonin acetylase (AANAT Antibody Abstract)
Background Arylalkylamine N-Acetyltransferase (AANAT) is the penultimate enzyme is melatoninsynthesis. Hormonal melatonin has been implicated in sleep and circadian clockfunction, and acts asthe hormone of the night. Large changes in theabundance of AANAT are believed to be responsible for the large day/night rhythms inmelatonin synthesis in the pineal gland and retina (1,2).Most research shows that AANAT activity is regulated by controlling the steady-statelevels of the protein. Recent research, however, suggests that another mechanism mayalso play a role in controlling melatonin production without altering AANAT protein andactivity. This mechanism may explain the sudden physiological increase in circulatingmelatonin observed at the onset of the dark period in humans (3). Alternate Names: anti-Arylalkamine N-Acetyltransferase antibody, anti-Serotonin-N-Acetyltransferaseantibody, anti-SNAT antibody.
Gene Symbol: AANAT
Gene ID 15, 25120
UniProt Q16613
Research Area Enzymes
Pathways
Application Notes This Serotonin N-acetyltransferase antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot. By Western blot, this antibody recognizes a band at 23 kDa.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:200, Western Blot 1:500-1:1000
Restrictions For Research Use only
Format Liquid
Buffer Eluted in 0.1N glacial acetic acid containing 0.1 % BSA & neutralized using 10x PBS, Thimerosal
Preservative Thimerosal (Merthiolate)
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
Immunohistochemistry (IHC) image for anti-Aralkylamine N-Acetyltransferase (AANAT) antibody (ABIN250114) AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber sl...
Western Blotting (WB) image for anti-Aralkylamine N-Acetyltransferase (AANAT) antibody (ABIN250114) Western blot analysis of hAANAT. Samples of 1E7 homogenates and bacterially expressed...
Product cited in: Klein, Ganguly, Coon et al.: "14-3-3 proteins in pineal photoneuroendocrine transduction: how many roles?" in: Journal of neuroendocrinology, Vol. 15, Issue 4, pp. 370-7, 2003 (PubMed).

Background publications Iuvone, Brown, Haque et al.: "Retinal melatonin production: role of proteasomal proteolysis in circadian and photic control of arylalkylamine N-acetyltransferase." in: Investigative ophthalmology & visual science, Vol. 43, Issue 2, pp. 564-72, 2002 (PubMed).