Egl Nine Homolog 1 (C. Elegans) (EGLN1) antibody

Details for Product No. ABIN250161
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Antigen
Synonyms EGLN1, egln2, phd2, zgc:92314, AI503754, C1orf12, HIF-PH2, HPH-2, Hif-p4h-2, ORF13, Phd2, SM-20, PHD-2, PHD2, ECYT3, HIFPH2, HPH2, SM20, ZMYND6
Reactivity
Human
(76), (27), (12), (12), (6), (1), (1), (1)
Host
Rabbit
(55), (29), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (5), (4), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunoelectron Microscopy (IEM), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(67), (18), (17), (13), (10), (10), (10), (7), (3), (3), (2)
Pubmed 5 references available
Quantity 0.025 mL
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Catalog No. ABIN250161
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Immunogen The epitope recognized by this antibody maps to a region between residues 1 and 50 of human PHD2/HIF Prolyl Hydroxylase 2 using the numbering given in entry NP_071334.1 (GeneID 54583).
No Cross-Reactivity Mouse (Murine)
Purification affinity purified
Alternative Name EGLN1 / PHD2
Background HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolylhydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIFis an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH isan essential component of the pathway through which cells sense oxygen. In thepresence of oxygen, HPHs convert specific prolyl residues in HIF-alpha tohydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at thecellular level, cause the HIF conversion to be reduced so that HIF is stable and there isincreased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIFalpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degradation via the vonHippel-Lindau ubiquitylation complex. Alternate Names: anti-C1ORF12 antibody, anti-PHD2 antibody, anti-Chromosome 1 Open Reading Frame12 antibody, anti-EGL 9 homolog of C. elegans 1 antibody, anti-EGLN 1 EGLN1antibody, anti-HIF Prolyl Hydroxylase 2 antibody, anti-HIF-P4H 2 antibody, anti-HIFPH2antibody, anti-P4H2 antibody, anti-Prolyl Hydroxylase Domain Containing antibody,anti-HPH-2 antibody, anti-PHD2 antibody, anti-EGLN1 antibody.
Gene Symbol: EGLN1
Gene ID 54583
NCBI Accession NP_071334
Application Notes This PHD2/HIF Prolyl Hydroxylase 2 antibody is useful for Flow Cytometry and Western blot, where a band can be seen at 46-50 kDa. Immunoprecipitation and Immunohistochemistry were reported in scientific literature. Use in Immunocytochemistry/immunofluorescence, Immunohistochemistry-Paraffin and Electron Microscopy reported in scientific literature (PMID 17003483)
Recommended dilutions: Electron Microscopy, Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot 1:500-1:2500
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/Phosphate, pH 7-8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
General Bruick, McKnight: "A conserved family of prolyl-4-hydroxylases that modify HIF." in: Science (New York, N.Y.), Vol. 294, Issue 5545, pp. 1337-40, 2001 (PubMed).

Ivan, Haberberger, Gervasi et al.: "Biochemical purification and pharmacological inhibition of a mammalian prolyl hydroxylase acting on hypoxia-inducible factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 21, pp. 13459-64, 2002 (PubMed).

Baek, Mahon, Oh et al.: "OS-9 interacts with hypoxia-inducible factor 1alpha and prolyl hydroxylases to promote oxygen-dependent degradation of HIF-1alpha." in: Molecular cell, Vol. 17, Issue 4, pp. 503-12, 2005 (PubMed).

Ietta, Wu, Winter et al.: "Dynamic HIF1A regulation during human placental development." in: Biology of reproduction, Vol. 75, Issue 1, pp. 112-21, 2006 (PubMed).

Colla, Tagliaferri, Morandi et al.: "The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 alpha ..." in: Blood, Vol. 110, Issue 13, pp. 4464-75, 2007 (PubMed).

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