Egl Nine Homolog 1 (C. Elegans) (EGLN1) antibody

Details for Product No. ABIN250161
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Synonyms C1orf12, ECYT3, HIF-PH2, HIFPH2, HPH-2, HPH2, PHD2, SM20, ZMYND6, AI503754, Hif-p4h-2, ORF13, Phd2, SM-20, zgc:92314, PHD-2, egln2, phd2, EGLN1
(65), (29), (12), (12), (6), (1), (1), (1)
(50), (24)
(6), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Flow Cytometry (FACS), Western Blotting (WB)
(58), (18), (16), (15), (10), (9), (6), (6), (5), (4), (2)
Pubmed 5 references available
Catalog no. ABIN250161
Quantity 25 µL
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Immunogen A synthetic peptide made to a C-terminal fragment of the human protein sequence ofHIF prolyl hydroxylase 2 (residues 350-426). [LocusLink ID 54583].
Specificity This antibody is specific for human HIF prolyl hydroxylase 2. There is 83% homologybetween the immunogen used for this antibody and the rat and mouse proteins. Species Reactivity: Human. Does not appear to work in mouse. Other species have not been tested.
Cross-Reactivity Human
Purification Affinity Purified
Alternative Name EGLN1 / PHD2
Background HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolylhydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIFis an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH isan essential component of the pathway through which cells sense oxygen. In thepresence of oxygen, HPHs convert specific prolyl residues in HIF-alpha tohydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at thecellular level, cause the HIF conversion to be reduced so that HIF is stable and there isincreased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIFalpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degradation via the vonHippel-Lindau ubiquitylation complex. Alternate Names: anti-C1ORF12 antibody, anti-PHD2 antibody, anti-Chromosome 1 Open Reading Frame12 antibody, anti-EGL 9 homolog of C. elegans 1 antibody, anti-EGLN 1 EGLN1antibody, anti-HIF Prolyl Hydroxylase 2 antibody, anti-HIF-P4H 2 antibody, anti-HIFPH2antibody, anti-P4H2 antibody, anti-Prolyl Hydroxylase Domain Containing antibody,anti-HPH-2 antibody, anti-PHD2 antibody, anti-EGLN1 antibody.
Gene ID 54583
Application Notes This antibody may be used for Western blotting where a band is at ~45-50 kDa,representing HIF prolyl hydroxylase 2. This antibody has been tested in humanglioblastoma cell lysates.Suggested working dilutions: *Western Blot 1:500-1:1,000 (ECL)Immunohistochemistry ND Immunoprecipitation ND
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/ml
Buffer Preservative: 0.1% sodium azide.
Preservative Sodium azide
Storage 4 °C
General Bruick, McKnight: "A conserved family of prolyl-4-hydroxylases that modify HIF." in: Science (New York, N.Y.), Vol. 294, Issue 5545, pp. 1337-40, 2001 (PubMed).

Ivan, Haberberger, Gervasi et al.: "Biochemical purification and pharmacological inhibition of a mammalian prolyl hydroxylase acting on hypoxia-inducible factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 21, pp. 13459-64, 2002 (PubMed).

Baek, Mahon, Oh et al.: "OS-9 interacts with hypoxia-inducible factor 1alpha and prolyl hydroxylases to promote oxygen-dependent degradation of HIF-1alpha." in: Molecular cell, Vol. 17, Issue 4, pp. 503-12, 2005 (PubMed).

Ietta, Wu, Winter et al.: "Dynamic HIF1A regulation during human placental development." in: Biology of reproduction, Vol. 75, Issue 1, pp. 112-21, 2006 (PubMed).

Colla, Tagliaferri, Morandi et al.: "The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 alpha (HIF-1alpha) activity: involvement in myeloma-induced angiogenesi" in: Blood, Vol. 110, Issue 13, pp. 4464-75, 2007 (PubMed).

Hosts (50), (24)
Reactivities (65), (29), (12), (12), (6), (1), (1), (1)
Applications (58), (18), (16), (15), (10), (9), (6), (6), (5), (4), (2)
Conjugates (6), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (10), (10), (6), (5), (5), (3), (2), (1), (1), (1), (1)
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