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Egl Nine Homolog 1 (C. Elegans) (EGLN1) (C-Term) antibody

Details for Product No. ABIN250161, Supplier: Log in to see
Antigen
  • AI503754
  • C1orf12
  • ECYT3
  • EGLN1
  • egln2
  • Hif-p4h-2
  • HIF-PH2
  • HIFPH2
  • HPH-2
  • HPH2
  • ORF13
  • PHD-2
  • phd2
  • Phd2
  • PHD2
  • SM-20
  • SM20
  • zgc:92314
  • ZMYND6
Epitope
C-Term
37
36
18
14
13
13
8
5
5
2
2
2
2
1
1
1
Reactivity
Human, Rat (Rattus)
148
58
38
13
2
1
1
Host
Rabbit
117
36
2
Clonality
Polyclonal
Conjugate
Un-conjugated
13
10
8
8
8
7
5
5
4
4
4
4
2
2
2
1
1
1
1
1
1
Application
Electron Microscopy (EM), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
128
65
41
34
33
31
26
19
12
5
2
1
1
1
1
Supplier
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Immunogen Synthetic peptide made to a C-terminal portion of human PHD2/HIF Prolyl Hydroxylase 2 (between amino acids 350-426) (GeneID 54583).
No Cross-Reactivity Mouse (Murine)
Purification Immunogen affinity purified
Alternative Name EGLN1/PHD2 (EGLN1 Antibody Abstract)
Background Gene Symbol: EGLN1
Molecular Weight Theoretical MW: 46 kDa
Gene ID 54583, 112405
UniProt Q9GZT9
Application Notes Western Blot 1:500-1:2500, Flow Cytometry 1:10-1:1000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Electron Microscopy
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/phosphate, pH 7-8
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) (C-Term) antibody (ABIN250161) Western blot analysis of human PHD2, using ABIN151072. Samples: Recombinant DYKDDDDK-...
Flow Cytometry (FACS) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) (C-Term) antibody (ABIN250161) Flow Cytometry: EGLN1/PHD2 Antibody [ABIN151072] - Flow cytometric detection of PHD2,...
Western Blotting (WB) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) (C-Term) antibody (ABIN250161) Western Blot: EGLN1/PHD2 Antibody [ABIN151072] - Detection of Human PHD2 by Western B...
Product cited in: Tan, Prosser, Dunn, Vanags, Ridiandries, Tsatralis, Leece, Clayton, Yuen, Robertson, Lam, Celermajer, Ng, Bursill: "High-Density Lipoproteins Rescue Diabetes-Impaired Angiogenesis via Scavenger Receptor Class B Type I." in: Diabetes, Vol. 65, Issue 10, pp. 3091-103, 2016 (PubMed).

Peurala, Koivunen, Bloigu, Haapasaari, Jukkola-Vuorinen: "Expressions of individual PHDs associate with good prognostic factors and increased proliferation in breast cancer patients." in: Breast cancer research and treatment, Vol. 133, Issue 1, pp. 179-88, 2012 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Serra-Pérez, Planas, Núñez-OMara, Berra, García-Villoria, Ribes, Santalucía: "Extended ischemia prevents HIF1alpha degradation at reoxygenation by impairing prolyl-hydroxylation: role of Krebs cycle metabolites." in: The Journal of biological chemistry, Vol. 285, Issue 24, pp. 18217-24, 2010 (PubMed). (Sample species: Human). Further details: Western Blotting

Mikheyev, Vo, Wee, Singer, Parmesan: "Rapid microsatellite isolation from a butterfly by de novo transcriptome sequencing: performance and a comparison with AFLP-derived distances." in: PLoS ONE, Vol. 5, Issue 6, pp. e11212, 2010 (PubMed).

Xie, Xiao, Whalen, Forrester, Freeman, Fong, Gygi, Lefkowitz, Stamler: "Oxygen-regulated beta(2)-adrenergic receptor hydroxylation by EGLN3 and ubiquitylation by pVHL." in: Science signaling, Vol. 2, Issue 78, pp. ra33, 2009 (PubMed).

Steinhoff, Pientka, Möckel, Kettelhake, Hartmann, Köhler, Depping: "Cellular oxygen sensing: Importins and exportins are mediators of intracellular localisation of prolyl-4-hydroxylases PHD1 and PHD2." in: Biochemical and biophysical research communications, Vol. 387, Issue 4, pp. 705-11, 2009 (PubMed). (Sample species: Human). Further details: Immunoprecipitation

Lehmann, Stiehl, Honer, Dominietto, Keist, Kotevic, Wollenick, Ametamey, Wenger, Rudin: "Longitudinal and multimodal in vivo imaging of tumor hypoxia and its downstream molecular events." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 33, pp. 14004-9, 2009 (PubMed).

Sakamoto, Seiki: "Mint3 enhances the activity of hypoxia-inducible factor-1 (HIF-1) in macrophages by suppressing the activity of factor inhibiting HIF-1." in: The Journal of biological chemistry, Vol. 284, Issue 44, pp. 30350-9, 2009 (PubMed).

Wong, Goehring, Kapiloff, Langeberg, Scott: "mAKAP compartmentalizes oxygen-dependent control of HIF-1alpha." in: Science signaling, Vol. 1, Issue 51, pp. ra18, 2008 (PubMed).

Colla, Tagliaferri, Morandi, Lunghi, Donofrio, Martorana, Mancini, Lazzaretti, Mazzera, Ravanetti, Bonomini, Ferrari, Miranda, Ladetto, Neri, Neri, Greco, Mangoni, Bonati, Rizzoli, Giuliani et al.: "The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 alpha ..." in: Blood, Vol. 110, Issue 13, pp. 4464-75, 2007 (PubMed).

Ietta, Wu, Winter, Xu, Wang, Post, Caniggia: "Dynamic HIF1A regulation during human placental development." in: Biology of reproduction, Vol. 75, Issue 1, pp. 112-21, 2006 (PubMed).

Cappuzzo, Toschi, Tallini, Ceresoli, Domenichini, Bartolini, Finocchiaro, Magrini, Metro, Cancellieri, Trisolini, Crino, Bunn, Santoro, Franklin, Varella-Garcia, Hirsch: "Insulin-like growth factor receptor 1 (IGFR-1) is significantly associated with longer survival in non-small-cell lung cancer patients treated with gefitinib." in: Annals of oncology : official journal of the European Society for Medical Oncology, Vol. 17, Issue 7, pp. 1120-7, 2006 (PubMed).

Khan, Michalopoulos, Stolz: "Peroxisomal localization of hypoxia-inducible factors and hypoxia-inducible factor regulatory hydroxylases in primary rat hepatocytes exposed to hypoxia-reoxygenation." in: The American journal of pathology, Vol. 169, Issue 4, pp. 1251-69, 2006 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Western Blotting,Immunohistochemistry (Paraffin-embedded Sections),Immunoelectron Microscopy,Immunofluorescence

Baek, Mahon, Oh, Kelly, Krishnamachary, Pearson, Chan, Giaccia, Semenza: "OS-9 interacts with hypoxia-inducible factor 1alpha and prolyl hydroxylases to promote oxygen-dependent degradation of HIF-1alpha." in: Molecular cell, Vol. 17, Issue 4, pp. 503-12, 2005 (PubMed). (Sample species: Human). Further details: Immunoprecipitation

Ivan, Haberberger, Gervasi, Michelson, Günzler, Kondo, Yang, Sorokina, Conaway, Conaway, Kaelin: "Biochemical purification and pharmacological inhibition of a mammalian prolyl hydroxylase acting on hypoxia-inducible factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 21, pp. 13459-64, 2002 (PubMed).

Background publications Bruick, McKnight: "A conserved family of prolyl-4-hydroxylases that modify HIF." in: Science (New York, N.Y.), Vol. 294, Issue 5545, pp. 1337-40, 2001 (PubMed).