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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Egl Nine Homolog 3 (C. Elegans) (EGLN3) antibody
|Synonyms||PHD3, HIFPH3, FLJ21620, MGC125998, MGC125999, Phd3, SM-20, AI505553, AI648162, 2610021G09Rik, zgc:77019, wu:fj78a08, EGLN3, PHD-3|
Alternatives Western Blotting (WB)
|10 references available|
|Quantity||25 µl (1 mg/ml) (Variants)|
|Price||Product not available in this region.|
|Alternative name||EGLN3 / PHD3|
|Immunogen||A synthetic peptide made to an internal fragment, which represented a portion of humanEGL9 homolog 3 encoded within exon 1. [LocusLink ID 112399].|
|Cross-Reactivity||Human, Rat (Rattus)|
|Description||HIF prolyl hydroxylase 3 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolylhydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIFis an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH isan essential component of the pathway through which cells sense oxygen. In thepresence of oxygen, HPHs convert specific prolyl residues in HIF-alpha tohydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at thecellular level, cause the HIF conversion to be reduced so that HIF is stable and there isincreased angiogenesis.HPH-1, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIFalpha proteins. It hydroxylates HIF-1 alpha at Pro(564) and HIF-2 alpha. It targets HIFthrough the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may also play a role in cell growth regulation in muscle cellsand in apoptosis in neuronal tissues, promoting cell death through a caspase-dependentmechanism. Alternate Names: anti-EGL9 homolog of C. elegans 3 antibody, anti-EGLN3 antibody, anti-Factorresponsive smooth muscle protein antibody, anti-HIF Prolyl Hydroxylase 3 antibody,anti-HIFP4H3 antibody, anti-HIFPH3 antibody, anti-P4H3 antibody, anti-ProlylHydroxylase Domain Containing Protein 3 antibody, anti-SM20 antibody, anti-PHD3antibody, anti-EGLN3 antibody.|
|Specificity||This antibody is specific for HIF prolyl hydroxylase 3. Species Reactivity: Human and Rat. Does not appear to work in Mouse.|
|Application Notes||This antibody may be used for Western blotting where a band is seen at ~27 kDa,representing HIF prolyl hydroxylase 3. This antibody has been tested in 293 cell lysates.Suggested working dilutions: *Western Blot 1:1,000-1:5000, Immunohistochemistry ND for this lotImmunoprecipitation ND for this lot|
|Purification||Aff - Purified|
|Buffer||Preservative: 0.09% Sodium Azide.|
|Storage||Store at 4C. Do not freeze.|
|Restrictions||For Research Use only|
Bruick, McKnight: "A conserved family of prolyl-4-hydroxylases that modify HIF." in: Science (New York, N.Y.), Vol. 294, Issue 5545, pp. 1337-40, 2001 (PubMed).
Ivan, Haberberger, Gervasi et al.: "Biochemical purification and pharmacological inhibition of a mammalian prolyl hydroxylase acting on hypoxia-inducible factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 21, pp. 13459-64, 2002 (PubMed).
Asikainen, Schneider, Waleh et al.: "Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 29, pp. 10212-7, 2005 (PubMed).
Jokilehto, Rantanen, Luukkaa et al.: "Overexpression and nuclear translocation of hypoxia-inducible factor prolyl hydroxylase PHD2 in head and neck squamous cell carcinoma is associated with tumor aggressiveness." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 12, Issue 4, pp. 1080-7, 2006 (PubMed).
Asikainen, Waleh, Schneider et al.: "Enhancement of angiogenic effectors through hypoxia-inducible factor in preterm primate lung in vivo." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 291, Issue 4, pp. L588-95, 2006 (PubMed).
Li, Yi, Sundy et al.: "Expression and actions of HIF prolyl-4-hydroxylase in the rat kidneys." in: American journal of physiology. Renal physiology, Vol. 292, Issue 1, pp. F207-16, 2007 (PubMed).
Fu, Menzies, Freeman et al.: "EGLN3 prolyl hydroxylase regulates skeletal muscle differentiation and myogenin protein stability." in: The Journal of biological chemistry, Vol. 282, Issue 17, pp. 12410-8, 2007 (PubMed).
Bai, Zeng, Hu et al.: "Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants." in: Biotechnology letters, Vol. 29, Issue 12, pp. 2007-12, 2007 (PubMed).
Rantanen, Pursiheimo, Hoegel et al.: "Prolyl hydroxylase PHD3 activates oxygen-dependent protein aggregation." in: Molecular biology of the cell, Vol. 19, Issue 5, pp. 2231-40, 2008 (PubMed).
Ginouvès, Ilc, Macías et al.: "PHDs overactivation during chronic hypoxia "desensitizes" HIFalpha and protects cells from necrosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 12, pp. 4745-50, 2008 (PubMed).
|Hosts||Rabbit (29), Goat (4)|
|Reactivities||Human (30), Mouse (Murine) (22), Rat (Rattus) (22), Cow (Bovine) (21), Dog (Canine) (19), Pig (Porcine) (18), Chicken (2), Cat (Feline) (1), Xenopus laevis (1)|
|Applications||Immunofluorescence (IF) (16), Western Blotting (WB) (16), ELISA (14), Immunohistochemistry (IHC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (3), Internal Region (2), AA 95-108 (1)|