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EGLN3 antibody (Egl Nine Homolog 3 (C. Elegans))

Details for Product anti-EGLN3 Antibody No. ABIN250181, Supplier: Log in to see
Antigen
  • zgc:77019
  • wu:fj78a08
  • EGLN3
  • phd3
  • PHD-3
  • PHD3
  • SM-20
  • 2610021G09Rik
  • AI505553
  • AI648162
  • Hif-p4h-3
  • Phd3
  • HIFP4H3
  • HIFPH3
  • egl nine homolog 3 (C. elegans)
  • egl nine homolog 3
  • EGL nine homolog 3 (C. elegans)
  • egl-9 family hypoxia-inducible factor 3
  • egln3
  • LOC100354210
  • EGLN3
  • LOC100219039
  • Egln3
Reactivity
Human, Mouse (Murine), Rat (Rattus)
126
57
47
24
8
8
7
6
3
3
2
2
2
2
1
Host
Rabbit
91
30
17
Clonality
Polyclonal
Conjugate
This EGLN3 antibody is un-conjugated
12
12
9
7
7
7
3
3
3
3
3
3
3
3
Application
Electron Microscopy (EM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
120
47
46
46
27
23
4
2
1
1
1
1
1
Options
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Immunogen Synthetic peptide corresponding to residues between 50-100 of human PHD3/HIF Prolyl Hydroxylase 3 using the numbering given in entry NP_071356.1 (GeneID 112399).
Purification Immunogen affinity purified
Alternative Name EGLN3/PHD3 (EGLN3 Antibody Abstract)
Background Gene Symbol: EGLN3
Molecular Weight Theoretical MW: 28 kDa
Gene ID 112399
UniProt Q9H6Z9
Application Notes Western Blot 1:1000-1:2000, Immunohistochemistry 1:250-1:1000, Immunocytochemistry/Immunofluorescence 1:500, Immunoprecipitation, Immunohistochemistry-Paraffin 1:250-1:1000, Electron MicroscopyIn WB, PHD3 band can be seen at 27-30 kDa molecular weight range. Use of this antibody in Electron Microscopy and ChIP is reported in scientific literature - PMIDs 17003483and 24367580 respectively. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-EGLN3 antibody (Egl Nine Homolog 3 (C. Elegans)) (ABIN250181) Detection of Human and Rat PHD3 by Western Blot. Samples: A. Lysate from cells that w...
Western Blotting (WB) image for anti-EGLN3 antibody (Egl Nine Homolog 3 (C. Elegans)) (ABIN250181) Western Blot: EGLN3/PHD3 Antibody [ABIN151073] - Detection of PHD3 by Western Blot ap...
Western Blotting (WB) image for anti-EGLN3 antibody (Egl Nine Homolog 3 (C. Elegans)) (ABIN250181) Western Blot: EGLN3/PHD3 Antibody [ABIN151073] - Analysis of HeLa lysates using ABIN1...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-EGLN3 antibody (Egl Nine Homolog 3 (C. Elegans)) (ABIN250181) Immunohistochemistry-Paraffin: EGLN3/PHD3 Antibody [ABIN151073] - Analysis of a forma...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-EGLN3 antibody (Egl Nine Homolog 3 (C. Elegans)) (ABIN250181) Immunohistochemistry-Paraffin: EGLN3/PHD3 Antibody [ABIN151073] - Analysis of a forma...
Immunofluorescence (IF) image for anti-EGLN3 antibody (Egl Nine Homolog 3 (C. Elegans)) (ABIN250181) Immunocytochemistry/Immunofluorescence: EGLN3/PHD3 Antibody [ABIN151073] - EGLN3/PHD3...
Product cited in: Vogler, Zieseniss, Hesse, Levent, Tiburcy, Heinze, Burzlaff, Schley, Eckardt, Willam, Katschinski: "Pre- and post-conditional inhibition of prolyl-4-hydroxylase domain enzymes protects the heart from an ischemic insult." in: Pflügers Archiv : European journal of physiology, Vol. 467, Issue 10, pp. 2141-9, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Li, Yuan, Jiang, Ye, Yang, Shapiro, Risbud: "Prolyl-4-hydroxylase domain protein 2 controls NF-κB/p65 transactivation and enhances the catabolic effects of inflammatory cytokines on cells of the nucleus pulposus." in: The Journal of biological chemistry, Vol. 290, Issue 11, pp. 7195-207, 2015 (PubMed). Further details: Western Blotting

Högel, Miikkulainen, Bino, Jaakkola: "Hypoxia inducible prolyl hydroxylase PHD3 maintains carcinoma cell growth by decreasing the stability of p27." in: Molecular cancer, Vol. 14, pp. 143, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Adam, Matt, Christian, Hess-Stumpp, Haegebarth, Hofmann, Algire: "SIAH ubiquitin ligases regulate breast cancer cell migration and invasion independent of the oxygen status." in: Cell cycle (Georgetown, Tex.), Vol. 14, Issue 23, pp. 3734-47, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Iriondo, Rábano, Domenici, Carlevaris, López-Ruiz, Zabalza, Berra, Vivanco: "Distinct breast cancer stem/progenitor cell populations require either HIF1α or loss of PHD3 to expand under hypoxic conditions." in: Oncotarget, Vol. 6, Issue 31, pp. 31721-39, 2015 (PubMed). Method employed by authors: Western Blotting (WB)

Rawluszko, Bujnicka, Horbacka, Krokowicz, Jagodziński: "Expression and DNA methylation levels of prolyl hydroxylases PHD1, PHD2, PHD3 and asparaginyl hydroxylase FIH in colorectal cancer." in: BMC cancer, Vol. 13, pp. 526, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Galán-Cobo, Sánchez-Silva, Serna, Abreu-Rodríguez, Muñoz-Cabello, Echevarría: "Cellular overexpression of aquaporins slows down the natural HIF-2α degradation during prolonged hypoxia." in: Gene, Vol. 522, Issue 1, pp. 18-26, 2013 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Taniguchi, Finger, Krieg, Wu, Diep, LaGory, Wei, McGinnis, Yuan, Kuo, Giaccia: "Cross-talk between hypoxia and insulin signaling through Phd3 regulates hepatic glucose and lipid metabolism and ameliorates diabetes." in: Nature medicine, Vol. 19, Issue 10, pp. 1325-30, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Place, Nauseef, Peterson, Henry, Mezhir, Domann: "Prolyl-4-hydroxylase 3 (PHD3) expression is downregulated during epithelial-to-mesenchymal transition." in: PLoS ONE, Vol. 8, Issue 12, pp. e83021, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunoprecipitation

Fujita, Markova, Anderson, Chiba, Toyama, Shapiro, Risbud et al.: "Expression of prolyl hydroxylases (PHDs) is selectively controlled by HIF-1 and HIF-2 proteins in nucleus pulposus cells of the intervertebral disc: distinct roles of PHD2 and PHD3 proteins in ..." in: The Journal of biological chemistry, Vol. 287, Issue 20, pp. 16975-86, 2012 (PubMed). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Di Fonzo, Pelletier, Clutton-Brock, Pemberton, Coulson: "The population growth consequences of variation in individual heterozygosity." in: PLoS ONE, Vol. 6, Issue 5, pp. e19667, 2011 (PubMed).

Anderson, Nordquist, Gao, Hicks, Zhai, Gygi, Patel: "Regulation of cellular levels of Sprouty2 protein by prolyl hydroxylase domain and von Hippel-Lindau proteins." in: The Journal of biological chemistry, Vol. 286, Issue 49, pp. 42027-36, 2011 (PubMed).

Place, Fitzgerald, Venkataraman, Vorrink, Case, Teoh, Domann: "Aberrant promoter CpG methylation is a mechanism for impaired PHD3 expression in a diverse set of malignant cells." in: PLoS ONE, Vol. 6, Issue 1, pp. e14617, 2011 (PubMed).

Luo, Hu, Chang, Zhong, Knabel, OMeally, Cole, Pandey, Semenza: "Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1." in: Cell, Vol. 145, Issue 5, pp. 732-44, 2011 (PubMed).

Högel, Rantanen, Jokilehto, Grenman, Jaakkola: "Prolyl hydroxylase PHD3 enhances the hypoxic survival and G1 to S transition of carcinoma cells." in: PLoS ONE, Vol. 6, Issue 11, pp. e27112, 2011 (PubMed). (Sample species: Human). Further details: Western Blotting

Luukkaa, Jokilehto, Kronqvist, Vahlberg, Grénman, Jaakkola, Minn: "Expression of the cellular oxygen sensor PHD2 (EGLN-1) predicts radiation sensitivity in squamous cell cancer of the head and neck." in: International journal of radiation biology, Vol. 85, Issue 10, pp. 900-8, 2010 (PubMed).

Schödel, Klanke, Weidemann, Buchholz, Bernhardt, Bertog, Amann, Korbmacher, Wiesener, Warnecke, Kurtz, Eckardt, Willam: "HIF-prolyl hydroxylases in the rat kidney: physiologic expression patterns and regulation in acute kidney injury." in: The American journal of pathology, Vol. 174, Issue 5, pp. 1663-74, 2009 (PubMed).

Chan, Kawahara, Sutphin, Chang, Chi, Giaccia: "Tumor vasculature is regulated by PHD2-mediated angiogenesis and bone marrow-derived cell recruitment." in: Cancer cell, Vol. 15, Issue 6, pp. 527-38, 2009 (PubMed).

Xie, Xiao, Whalen, Forrester, Freeman, Fong, Gygi, Lefkowitz, Stamler: "Oxygen-regulated beta(2)-adrenergic receptor hydroxylation by EGLN3 and ubiquitylation by pVHL." in: Science signaling, Vol. 2, Issue 78, pp. ra33, 2009 (PubMed).

Rantanen, Pursiheimo, Högel, Himanen, Metzen, Jaakkola: "Prolyl hydroxylase PHD3 activates oxygen-dependent protein aggregation." in: Molecular biology of the cell, Vol. 19, Issue 5, pp. 2231-40, 2008 (PubMed).