Peptidyl Arginine Deiminase, Type IV (PADI4) (N-Term), (AA 2-15) antibody

Details for Product No. ABIN250318
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Antigen
Synonyms PAD, PAD4, PADI5, PDI4, PDI5, Pad4, Pdi4
Epitope
N-Term, AA 2-15
(14), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(62), (8), (8), (1), (1), (1), (1), (1), (1)
Host
Goat
(45), (10), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA
(51), (22), (16), (11), (10), (9), (8), (5), (3), (1), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
Shipping to United States (Change)
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Catalog No. ABIN250318
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Immunogen Synthetic peptide AQGTLIRVTPEQPC, representing the N-Terminus of the human protein (residues 2-15), according to NP_036519.
Isotype IgG
Purification affinity purified
Alternative Name PADI4
Background In vivo expression of PADI4 correlated with the appearance of citrulline in histones. Wehave shown that PADI4 converts arginines in the histone H3 tail to citrulline, that PADI4blocks transcriptional activation and is recruited to the endogenous promotor. PADI4represents a novel class of histone modifying enzymes probably linked to eostrogenregulated transcription. Alternate Names: anti-EC 3.5.3.15 antibody, anti-HL 60 PAD antibody, anti-PADI5 antibody, anti-PDI5antibody, anti-Peptidylarginine deiminase IV antibody, anti-Protein arginine deiminasetype IV antibody. Related Diseases: Rheumatoid Arthritis
Gene Symbol: PADI4
Gene ID 23569
NCBI Accession NP_036519
Application Notes Approx 74kDa band observed in human spleen and human liver lysates (calculated MW of 74.1kDa according to NP_036519).
Recommended dilutions: Peptide ELISA detection limit 1:64000, Western Blot 1:100-1:2000
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris-saline (pH 7.3) with 0.5 % BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
anti-Peptidyl Arginine Deiminase, Type IV (PADI4) (N-Term), (AA 2-15) antibody ABIN250318 (0.3µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Wang, Wysocka, Sayegh et al.: "Human PAD4 regulates histone arginine methylation levels via demethylimination." in: Science (New York, N.Y.), Vol. 306, Issue 5694, pp. 279-83, 2004 (PubMed).

Liu, Mani, Davis et al.: "Mutation in EGFP domain of LDL receptor-related protein 6 impairs cellular LDL clearance." in: Circulation research, Vol. 103, Issue 11, pp. 1280-8, 2008 (PubMed).

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