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Peptidyl Arginine Deiminase, Type IV (PADI4) antibody

Antigen

Peptidyl Arginine Deiminase, Type IV (PADI4)
Synonyms Pdi4, PAD, PAD4, PDI4, PDI5, PADI5
Clonality Polyclonal
Host
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), ELISA
2 references available
Catalog no. ABIN250318
Quantity 0.1 mg  (0.5 mg/ml)
Price Product not available in this region.
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anti-Peptidyl Arginine Deiminase, Type IV (PADI4) antibody

Additional Information
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Alternative name PADI4
Gene ID 23569
NCBI Acession NP_036519
Immunogen Synthetic peptide AQGTLIRVTPEQPC, representing the N-Terminus of the humanprotein (residues 2-15), according to NP_036519. Reference Sequence: NP_036519.
Cross-Reactivity Human
Isotype IgG
Description In vivo expression of PADI4 correlated with the appearance of citrulline in histones. Wehave shown that PADI4 converts arginines in the histone H3 tail to citrulline, that PADI4blocks transcriptional activation and is recruited to the endogenous promotor. PADI4represents a novel class of histone modifying enzymes probably linked to eostrogenregulated transcription. Alternate Names: anti-EC 3.5.3.15 antibody, anti-HL 60 PAD antibody, anti-PADI5 antibody, anti-PDI5antibody, anti-Peptidylarginine deiminase IV antibody, anti-Protein arginine deiminasetype IV antibody. Related Diseases: Rheumatoid Arthritis
Specificity Species Reactivity: Reacts with Human. Not yet tested in other species.

Application Details
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Application Notes This antibody can be used for ELISA and Western Blot. In Western Blot, approx 74kDaband observed in Human Spleen and Human Liver lysates (calculated MW of 74.1kDaaccording to NP_036519). Recommended Starting Dilution:*ELISA (peptide): 1:64,000WB: 0.3-2ug/ml
Concentration 0.5 mg/ml
Purification Aff - Purified
Buffer Tris-saline containing 0.02% Na azide [pH7.3] with 0.5% BSA
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.
Restrictions For Research Use only

Publications
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Wang, Wysocka, Sayegh et al.: "Human PAD4 regulates histone arginine methylation levels via demethylimination." in: Science (New York, N.Y.), Vol. 306, Issue 5694, pp. 279-83, 2004 (PubMed).

Liu, Mani, Davis et al.: "Mutation in EGFP domain of LDL receptor-related protein 6 impairs cellular LDL clearance." in: Circulation research, Vol. 103, Issue 11, pp. 1280-8, 2008 (PubMed).

Alternatives
Application Details Publications Images back to top

Alternatives for antigen "Peptidyl Arginine Deiminase, Type IV (PADI4)", type "Antibodies"
Hosts Rabbit (38), Goat (4), Mouse (2)
Reactivities Human (43), Mouse (Murine) (8), Rat (Rattus) (6), Chicken (1), Chimpanzee (1), Cow (Bovine) (1), Dog (Canine) (1), Xenopus laevis (1), Zebrafish (1)
Applications Western Blotting (WB) (27), ELISA (21), Immunofluorescence (IF) (19), Immunoprecipitation (IP) (7), Immunohistochemistry (IHC) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Flow Cytometry (FACS) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes C-Term (5), N-Term (4), AA 200-250,Internal Region (1), Internal Region (1)