CD2 (Cytoplasmic Tail) Binding Protein 2 (CD2BP2) antibody

Antigen: CD2 (Cytoplasmic Tail) Binding Protein 2 (CD2BP2)

Synonyms: LIN1, Snu40, FWP010, U5-52K

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN250400

Additional Information

Alternative name: CD2BP2

Gene ID: 10421, 70233

NCBI Acession: NP_0061011

Immunogen: Synthetic peptide with sequence PKRKVTFQGVGDE-C, representing the N Terminus ofthe human protein according to NP_006101.1. Reference Sequence: NP_006101.1.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG

Description: CD2BP2 is an intracellular protein which binds to a site containing two PPPGHRsegments within the cytoplasmic region of CD2. Mutagenesis and NMR analysisdemonstrated that the CD2 binding region of CD2BP2 includes a 17 amino acid motifalso found in several yeast and Caenorhabditis elegans proteins of unknown function. InJurkat T cells, over expression of the isolated CD2BP2 domain binding to CD2enhances the production of interleukin 2 on crosslinking of CD2 but not the T cellreceptor. Alternate Names: anti-CD2 (cytoplasmic tail) binding protein 2 antibody, anti-CD2 antigen (cytoplasmictail) binding protein 2 antibody, anti-FWP010 antibody, anti-LIN1 antibody, anti-Snu40antibody.

Specificity: This antibody is specific for CD2BP2. Species Reactivity: Cross reacts with Human, Mouse and Rat.

Application Details

Application Notes: Suggested working dilutions: ELISA 1:32,000,Western Blot 0.3-1.0ug/ml

Concentration: 0.5 mg/ml

Purification: Aff - Purified

Buffer: Tris saline containing 0.02% sodium azide, pH7.3 with 0.5% BSA

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Research Area: Immunology, Adaptive Immunity, CD Antigens

Restrictions: For Research Use only

Images

Western Blot: CD2BP2 Antibody [ABIN250398] - ABIN250400 (0.3ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Nishizawa, Freund, Li et al.: "Identification of a proline-binding motif regulating CD2-triggered T lymphocyte activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 25, pp. 14897-902, 1999 (PubMed).