Reticulon 4 Receptor (RTN4R) (Internal Region) antibody

Details for Product No. ABIN250419
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms NgR, NGR, NOGOR, NgR1, Nogor
Epitope
Internal Region
(4), (3), (2), (1), (1), (1)
Reactivity
Human
(15), (5), (3), (2)
Host
Goat
(8), (5), (3), (2)
Clonality
Polyclonal
Application
Western Blotting (WB), ELISA
(15), (5), (4), (2), (2)
Pubmed 1 reference available
Quantity 0.1 mg
Shipping to United States (Change)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN250419
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Synthetic peptide with sequence C-LQALPDDTFRDLGN, representing the internal region of the human protein according to NP_075380.
Isotype IgG
Cross-Reactivity (Details) Expected cross reactivity from sequence similarity: Mouse, Rat, Pig,Dog.
Purification affinity purified
Alternative Name Nogo 66 receptor
Background This gene encodes the receptor for reticulon 4, oligodendrocyte myelin glycoprotein andmyelin-associated glycoprotein. This receptor mediates axonal growth inhibition andmay play a role in regulating axonal regeneration and plasticity in the adult centralnervous system. Alternate Names: anti-RTN4R antibody, anti-reticulon 4 receptor antibody, anti-HGNC antibody, anti-1860antibody, anti-NGR antibody, anti-NOGOR antibody, anti-Nogo-66 receptor antibody,anti-UNQ330/PRO526 antibody, anti-nogo receptor antibody, anti-reticulon 4 receptorprecursor antibody.
Gene Symbol: RTN4R
Gene ID 65078
NCBI Accession NP_075380
Application Notes Experiments gave bands at approx 90-100kDa and 37+40+45kDa in Human brain lysate after antibody staining. These bands were successfully blocked by the immunizing peptide and they correspond to earlier findings in the literature with different antibodies (Wang et al, J Neurosci. 2002 Jul 1,22(13):5505-15). This protein has a calculated MW of 50.7kDa according to NP_075380.1.
Recommended dilutions: Peptide ELISA detection limit 1:16000, Western Blot 1:100-1:2000
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 with 0.5 % BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
anti-Reticulon 4 Receptor (RTN4R) (Internal Region) antibody Western Blot: Nogo 66 receptor Antibody [ABIN250419] - ABIN250419 (0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
General Shao, Browning, Lee et al.: "TAJ/TROY, an orphan TNF receptor family member, binds Nogo-66 receptor 1 and regulates axonal regeneration." in: Neuron, Vol. 45, Issue 3, pp. 353-9, 2005 (PubMed).

Validation Images
Did you look for something else?
back to top