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Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) (AA 400-502), (C-Term) antibody

Details for Product No. ABIN250437, Supplier: Log in to see
Antigen
  • Ar
  • ArKO
  • ARO
  • aro
  • ARO1
  • Aromatase
  • CPV1
  • CYAR
  • CYP19
  • Cyp19
  • cyp19
  • Cyp19a
  • cyp19a
  • CYP19A1
  • CYP19P1
  • CYPXIX
  • Int-5
  • Int5
  • P-450AROM
  • P450 arom
  • p450arom
  • P450AROM
  • p450arom-A
Epitope
C-Term, AA 400-502
33
20
17
14
10
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Primate, Rabbit
143
56
39
33
13
9
8
8
7
4
4
3
3
1
1
1
1
1
1
1
Host
Rabbit
101
29
19
Clonality
Polyclonal
Conjugate
Un-conjugated
6
5
5
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
116
41
39
36
22
22
10
4
3
2
Supplier
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Immunogen A synthetic peptide made to a C-terminal portion of the human Aromatase protein (between residues 400-502). [UniProt# P11511]
Purification Immunogen affinity purified
Alternative Name Aromatase (CYP19A1 Antibody Abstract)
Background Gene Symbol: CYP19A1
Molecular Weight Theoretical MW: 55 kDa
Gene ID 1588
UniProt P11511
Research Area Cancer, Endocrine system, Enzymes
Application Notes Western Blot 1 μg/mL, Immunocytochemistry/Immunofluorescence 1:100
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Aromatase Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 20 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 2 hours at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-Aromatase primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce's ECL). Viewed band in 1-2 minutes of exposure.Note: Tween-20 can be added to the blocking or antibody diultion buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.Immunocytochemistry Protocol Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1.2 mg/mL
Buffer Tris-citrate/phosphate, pH 7-8
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) (AA 400-502), (C-Term) antibody (ABIN250437) Detection of aromatasein human fetal temporal lobe lysate using ABIN151143. ECL expos...
Immunofluorescence (IF) image for anti-Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) (AA 400-502), (C-Term) antibody (ABIN250437) Immunocytochemistry/Immunofluorescence: Aromatase Antibody [ABIN151143] - Aromatase a...
Product cited in: de los Ángeles Carrasco-Ruiz, García-Villamar, López-García, Sánchez-García, Pacheco, Cuevas, Martínez-Gómez, Castelán: "Aromatase expression is linked to estrogenic sensitivity of periurethral muscles in female rabbits." in: Cell biochemistry and function, Vol. 33, Issue 4, pp. 188-95, 2015 (PubMed). (Sample species: Rabbit). Further details: Western Blotting

Pignatti, Casarini, Scaltriti, Wistuba, Schlatt, Rossi, Lachhab, Taliani, Carani, Simoni: "Aromatase expression in human peripheral blood leucocytes (PBLs) and in various tissues in primates: studies in elderly humans and cynomolgus monkeys." in: Journal of medical primatology, Vol. 41, Issue 6, pp. 372-83, 2012 (PubMed). Further details: Western Blotting

Bogan, Murphy, Hennebold: "Dynamic changes in gene expression that occur during the period of spontaneous functional regression in the rhesus macaque corpus luteum." in: Endocrinology, Vol. 150, Issue 3, pp. 1521-9, 2009 (PubMed).

Bogan, Murphy, Stouffer, Hennebold: "Systematic determination of differential gene expression in the primate corpus luteum during the luteal phase of the menstrual cycle." in: Molecular endocrinology (Baltimore, Md.), Vol. 22, Issue 5, pp. 1260-73, 2008 (PubMed). (Sample species: Primate). Further details: Western Blotting

Background publications Beyer, Green, Barker, Huskisson, Hutchison: "Aromatase-immunoreactivity is localised specifically in neurones in the developing mouse hypothalamus and cortex." in: Brain research, Vol. 638, Issue 1-2, pp. 203-10, 1994 (PubMed).