X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3) antibody

Antigen: X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN250464

Additional Information

Alternative name: XRCC3

Gene ID: 7517

Immunogen: A synthetic partial peptide of human XRCC3.

Description: Five human RAD51 homologs have been identified: XRCC2, XRCC3, RAD51B,RAD51C, and RAD51D. Each of these homolgs interacts with one or more of theothers, with all of the proteins involved in one complex or multiple smaller complexes.XRCC3 (x-ray cross complementing) was first identified as a human genecomplementing the DNA damage sensitivity, chromosonal instability and impairedgrowth of the mutant hamster cell line irs1SF. This fact coupled with XRCC3 being amember of the RAD51 gene family have implied a role in the repair of DNA damage byhomologous recombination. Alternate Names: anti-DNA repair protein XRCC3 antibody, anti-X ray repair complementing defectiverepair in Chinese hamster cells 3 antibody, anti-X ray repair cross complementingprotein 3 antibody.

Specificity: This antibody is specific for XRCC3. Species Reactivity: 100-165 reacts with human XRCC3. Other species have not been tested. Localization: Nuclear.

Application Details

Application Notes: Suggested working dilutions: Western Blot 1:5000. Positive Controls: TK6 lysates and 100 ug HeLa lysates (NB 800-PC1, whole cell)

Buffer: PBS. Preservative: 0.05% sodium azide.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Research Area: Chromatin and Nuclear Signaling, Chromatin Binding Proteins, DNA/RNA

Restrictions: For Research Use only

Images

Western blot detection of XRCC3 (38 kDa) from Hela cell extract using ABIN151186 (1:2500). Lanes 1 and 2 contain HTC116 and Hela whole cell extracts, respectively.

Publications

Publications: Yoshihara, Ishida, Kinomura et al.: "XRCC3 deficiency results in a defect in recombination and increased endoreduplication in human cells." in: The EMBO journal, Vol. 23, Issue 3, pp. 670-80, 2004 (PubMed).