RAD51D antibody (RAD51 Homolog D)

Details for Product anti-RAD51D Antibody No. ABIN250467, Supplier: Log in to see
Antigen
  • R51H3
  • Rad51l3
  • TRAD
  • RAD51 homolog D
  • Rad51d
Reactivity
Human
112
15
7
7
1
1
Host
Rabbit
52
48
14
Clonality
Polyclonal
Conjugate
This RAD51D antibody is un-conjugated
7
7
6
5
5
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
97
30
22
14
13
4
1
1
1
Options
Supplier
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Immunogen A peptide from human Rad51D (homologue 4). [UniProt# O75771]
Specificity This is specific for hRAD51D. There is no cross-reactivity observed with XRCC2, XRCC3, RAD51, RAD51B, or RAD51C.
No Cross-Reactivity Mouse (Murine)
Purification Immunogen affinity purified
Alternative Name Rad51D (RAD51D Antibody Abstract)
Background Gene Symbol: RAD51D
Molecular Weight Theoretical MW: 35 kDa
Gene ID 5892
UniProt O75771
Research Area DNA/RNA, Chromatin and Nuclear Signaling
Application Notes Western Blot 1:1000, Immunocytochemistry/Immunofluorescence, ImmunoprecipitationThis Rad51D antibody is useful for Western blot, where it recognizes a band at 35 kDa representing Rad51D (H3 splice variant). Immunocytochemistry/Immunofluorescence and Immunoprecipitation were reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-RAD51 Homolog D (RAD51D) antibody (ABIN250467) Western blot detection of Rad51D (35 kDa) from Hela S3 cell extract using ABIN151188.
Product cited in: Park, Virts, Jankowska, Wiek, Othman, Chakraborty, Vance, Alkuraya, Hanenberg, Andreassen: "Complementation of hypersensitivity to DNA interstrand crosslinking agents demonstrates that XRCC2 is a Fanconi anaemia gene." in: Journal of medical genetics, Vol. 53, Issue 10, pp. 672-80, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Huang, Wang, Dhillon, Calses, Villegas, Mitchell, Tewari, Kemp, Taniguchi: "Systematic screen identifies miRNAs that target RAD51 and RAD51D to enhance chemosensitivity." in: Molecular cancer research : MCR, Vol. 11, Issue 12, pp. 1564-73, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Wang, Huang, Calses, Kemp, Taniguchi: "MiR-96 downregulates REV1 and RAD51 to promote cellular sensitivity to cisplatin and PARP inhibition." in: Cancer research, Vol. 72, Issue 16, pp. 4037-46, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Chun, Buechelmaier, Powell: "Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway." in: Molecular and cellular biology, Vol. 33, Issue 2, pp. 387-95, 2012 (PubMed). (Sample species: Human).

Shammas, Shmookler Reis, Koley, Batchu, Li, Munshi: "Dysfunctional homologous recombination mediates genomic instability and progression in myeloma." in: Blood, Vol. 113, Issue 10, pp. 2290-7, 2009 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Chattopadhyay, Das, Maiti, Boldogh, Xie, Hazra, Kohno, Mitra, Bhakat: "Regulatory role of human AP-endonuclease (APE1/Ref-1) in YB-1-mediated activation of the multidrug resistance gene MDR1." in: Molecular and cellular biology, Vol. 28, Issue 23, pp. 7066-80, 2008 (PubMed).

Hinz, Tebbs, Wilson, Nham, Salazar, Nagasawa, Urbin, Bedford, Thompson: "Repression of mutagenesis by Rad51D-mediated homologous recombination." in: Nucleic acids research, Vol. 34, Issue 5, pp. 1358-68, 2006 (PubMed).

Wiese, Hinz, Tebbs, Nham, Urbin, Collins, Thompson, Schild: "Disparate requirements for the Walker A and B ATPase motifs of human RAD51D in homologous recombination." in: Nucleic acids research, Vol. 34, Issue 9, pp. 2833-43, 2006 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunoprecipitation

Schild, Lio, Collins, Tsomondo, Chen: "Evidence for simultaneous protein interactions between human Rad51 paralogs." in: The Journal of biological chemistry, Vol. 275, Issue 22, pp. 16443-9, 2000 (PubMed).

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