Cathepsin F (CTSF) (C-Term) antibody

Details for Product No. ABIN250491
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Synonyms CTSF, MGC140519, cb420, sb:cb420, zgc:153093, LOC100101596, Ctsf, ACYPI000376, catf, CATSF, CLN13, AI481912
(14), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
(53), (9), (5), (4), (1), (1), (1), (1)
(33), (17), (4), (1)
(5), (5), (5), (1), (1), (1)
Western Blotting (WB), ELISA
(51), (36), (8), (3), (2), (1), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN250491
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Immunogen Synthetic peptide: CGVNTMASSAVVD, representing the C Terminus of the human protein according to NP_003784.
Isotype IgG
Cross-Reactivity (Details) Expected cross reactivity from sequence similarity: Mouse, Rat,Dog.
Purification affinity purified
Alternative Name Cathepsin F
Background Cathepsins are papain family cysteine proteinases that represent a major component ofthe lysosomal proteolytic system. Cathepsins generally contain a signal sequence,followed by a propeptide and then a catalytically active mature region. The very long(251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminaldomain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexiblelinker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. Thecathepsin F proregion is unique within the papain family cysteine proteases in that itcontains this additional N-terminal segment predicted to share structural similarities withcysteine protease inhibitors of the cystatin superfamily. This cystatin-like domaincontains some of the elements known to be important for inhibitory activity. CTSFencodes a predicted protein of 484 amino acids which contains a 19 residue signalpeptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targetedto the endosomal/lysosomal compartment via the mannose 6-phosphate receptorpathway. The cathepsin F gene is ubiquitously expressed, and it maps to chromosome11q13, close to the gene encoding cathepsin W. Alternate Names: anti-CTSF antibody, anti-cathepsin F antibody, anti-HGNC antibody, anti-2531 antibody,anti-CATSF antibody.
Gene Symbol: CTSF
Gene ID 8722
NCBI Accession NP_003784
Research Area Immunology, Innate Immunity, Proteases
Application Notes Experiments gave bands at approx 30kDa and 35kDa in Human Prostate and Ovary lysates after antibody staining. These bands correspond to earlier findings in literature with different antibodies (Wang et al, J Biol Chem. 1998 Nov 27,273(48):32000-8, PMID: 9822672). This protein has a calculated MW of 53.4kDa according to NP_003784.2.
Recommended dilutions: Peptide ELISA detection limit 1:32000, Western Blot 0.3 µg/mL
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris-saline (pH 7.3) with 0.5 % BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
anti-Cathepsin F (CTSF) (C-Term) antibody Western Blot: Cathepsin F Antibody [ABIN250491] - ABIN250491 (0.3ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Maubach, Lim, Zhuo: "Nuclear cathepsin F regulates activation markers in rat hepatic stellate cells." in: Molecular biology of the cell, Vol. 19, Issue 10, pp. 4238-48, 2008 (PubMed).

Dorn, Zhao, Granberg et al.: "Identification of specific cellular genes up-regulated late in adenovirus type 12 infection." in: Journal of virology, Vol. 79, Issue 4, pp. 2404-12, 2005 (PubMed).

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