Cathepsin F (CTSF) antibody

Antigen: Cathepsin F (CTSF)

Synonyms: CATSF, AI481912, MGC124702, CTSF, MGC140519, cb420, sb:cb420, MGC153093, zgc:153093, LOC100101596, Ctsf, ACYPI000376, catf

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN250491

Additional Information

Alternative name: Cathepsin F

Gene ID: 8722

NCBI Acession: NP_0037842

Immunogen: Synthetic peptide: CGVNTMASSAVVD, representing the C Terminus of the humanprotein according to NP_003784.2. Reference Sequence: NP_003784.2.

Cross-Reactivity: Human

Isotype: IgG

Description: Cathepsins are papain family cysteine proteinases that represent a major component ofthe lysosomal proteolytic system. Cathepsins generally contain a signal sequence,followed by a propeptide and then a catalytically active mature region. The very long(251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminaldomain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexiblelinker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. Thecathepsin F proregion is unique within the papain family cysteine proteases in that itcontains this additional N-terminal segment predicted to share structural similarities withcysteine protease inhibitors of the cystatin superfamily. This cystatin-like domaincontains some of the elements known to be important for inhibitory activity. CTSFencodes a predicted protein of 484 amino acids which contains a 19 residue signalpeptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targetedto the endosomal/lysosomal compartment via the mannose 6-phosphate receptorpathway. The cathepsin F gene is ubiquitously expressed, and it maps to chromosome11q13, close to the gene encoding cathepsin W. Alternate Names: anti-CTSF antibody, anti-cathepsin F antibody, anti-HGNC antibody, anti-2531 antibody,anti-CATSF antibody.

Specificity: Species Reactivity: Cross-reacts with Human. Expected to cross-react with Mouse, Dog. Not yet tested inother species.

Application Details

Application Notes: Positive Controls: Human Prostate and Ovary lysates

Concentration: 0.5 mg/ml

Purification: Aff - Purified

Buffer: Tris-saline containing 0.02% Na azide (pH7.3) with 0.5% BSA

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Research Area: Immunology, Innate Immunity, Proteases

Restrictions: For Research Use only

Images

Western Blot: Cathepsin F Antibody [ABIN250491] - ABIN250491 (0.3ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Dorn, Zhao, Granberg et al.: "Identification of specific cellular genes up-regulated late in adenovirus type 12 infection." in: Journal of virology, Vol. 79, Issue 4, pp. 2404-12, 2005 (PubMed).

Maubach, Lim, Zhuo: "Nuclear cathepsin F regulates activation markers in rat hepatic stellate cells." in: Molecular biology of the cell, Vol. 19, Issue 10, pp. 4238-48, 2008 (PubMed).