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RAD51D antibody (RAD51 Homolog D)

Details for Product anti-RAD51D Antibody No. ABIN250499, Supplier: Log in to see
  • R51H3
  • Rad51l3
  • TRAD
Clonality (Clone)
Monoclonal ()
This RAD51D antibody is un-conjugated
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
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Immunogen His-tagged human Rad51D, overexpressed in E. coli.
Clone 5B3-6
Isotype IgG1
No Cross-Reactivity Mouse (Murine)
Purification Ascites
Alternative Name RAD51L3 (RAD51D Antibody Abstract)
Background Five human RAD51 homologues have been identified: XRCC2, XRCC3, RAD51B,RAD51C, and RAD51D. Each of these homologues interacts with one or more of theothers, with all of the proteins involved in one complex or multiple smaller complexes. RAD51D interacts with XRCC2 and RAD51C (1). RAD51D's expression pattern andsequence similarity to other RAD51 family members most likely make it part of acomplex of proteins required for DNA repair and meiotic recombination. Alternate Names: anti-RAD51L3 antibody, anti-RAD51-like 3 (S. cerevisiae) antibody, anti-TRAD antibody,anti-R51H3 antibody, anti-HsTRAD antibody, anti-RAD51D antibody, anti-recombinationrepair protein antibody, anti-DNA repair protein RAD51 homolog 4 antibody.
Gene Symbol: RAD51D
Gene ID 5892, 19364
UniProt O75771
Application Notes This Rad51D (5B3/6) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~40 kDa.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:100-1:500, Western Blot 1:1000
Protocol Western Blot protocol specific for Rad51D Antibody Western Blot
1. Preparation of samples for loading gel: Heat ~50-80ug of sample containing laemmli loading dye (containing SDS) at 90C for ~2 minutes.
. Load sample onto a 10 % Tris-HCL gel (Bio-Rad pre-cast) and run for ~30 minutes at 200V (or until dye front reaches bottom of gel).
. Place gel in transfer buffer for 10 minutes (192mM Glycine, 25mM Tris-HCL, 20 % Methanol). Pre-soak two pieces of Whatman paper and PVDF, as well.NOTE: The PVDF should be soaked in CH3OH for ~ 1minute, rinsed in ddH20 and then placed in transfer buffer.
. Transfer the protein from the gel to the membrane using a semi-dry transfer apparatus. Run for 20 minutes at 20V.
. Block non-specific proteins with blocking buffer #1 (10mM Tris-HCL pH 8.0, 300mM NaCL, 0.025 % Tween 20)for 10 minutes. Then continue blocking in blocking buffer #2 (buffer #1 + 15 % nonfat dry milk)for an additional hour, gently rocking at room temperature (RT) or overnight at 4C.
. Dilute the primary antibody (anti-Rad51D, NB 100-178) in antibody dilution buffer (blocking buffer #1 + 2 % milk).
. Wash the membrane briefly with some blocking buffer #1 and then add your diluted primary antibody.
. Incubate the primary for 1 hour at room temperature, gently rocking. Again this can be done overnight at 4C.
. Wash 3X with blocking buffer #1 for 10 minutes, each, gently rocking.
. Incubate the diluted secondary antibody (anti-mouse IgG conjugated to HRP), diluted in antibody dilution buffer, for1 hour at room temperature, gently rocking.
. Wash 2X with blocking buffer #1 for 10 minutes, each, gently rocking. Wash 1X with blocking buffer #1 for 30 minutes, gently rocking.
. Develop membrane with your chemiluminescent substrate.NOTE: HEK 293 and MCF-7 whole cell extracts have been used as positive controls for this antibody.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-RAD51D antibody (RAD51 Homolog D) (ABIN250499) Rad51D detected in HEK293 lysates using a 1:1,000 dilution of ABIN151303 (purified). ...
Product cited in: Shammas, Shmookler Reis, Koley et al.: "Dysfunctional homologous recombination mediates genomic instability and progression in myeloma." in: Blood, Vol. 113, Issue 10, pp. 2290-7, 2009 (PubMed).

Hinz, Tebbs, Wilson et al.: "Repression of mutagenesis by Rad51D-mediated homologous recombination." in: Nucleic acids research, Vol. 34, Issue 5, pp. 1358-68, 2006 (PubMed).

Fan, Kumaravel, Jalali et al.: "Defective DNA strand break repair after DNA damage in prostate cancer cells: implications for genetic instability and prostate cancer progression." in: Cancer research, Vol. 64, Issue 23, pp. 8526-33, 2004 (PubMed).

Lambert, Lopez: "Inactivation of the RAD51 recombination pathway stimulates UV-induced mutagenesis in mammalian cells." in: Oncogene, Vol. 21, Issue 25, pp. 4065-9, 2002 (PubMed).

Maacke, Hundertmark, Miska et al.: "Autoantibodies in sera of pancreatic cancer patients identify recombination factor Rad51 as a tumour-associated antigen." in: Journal of cancer research and clinical oncology, Vol. 128, Issue 4, pp. 219-22, 2002 (PubMed).

Masson, Tarsounas, Stasiak et al.: "Identification and purification of two distinct complexes containing the five RAD51 paralogs." in: Genes & development, Vol. 15, Issue 24, pp. 3296-307, 2001 (PubMed).

Background publications Kawabata, Saeki: "Multiple alternative transcripts of the human homologue of the mouse TRAD/R51H3/RAD51D gene, a member of the rec A/RAD51 gene family." in: Biochemical and biophysical research communications, Vol. 257, Issue 1, pp. 156-62, 1999 (PubMed).

Pittman, Weinberg, Schimenti: "Identification, characterization, and genetic mapping of Rad51d, a new mouse and human RAD51/RecA-related gene." in: Genomics, Vol. 49, Issue 1, pp. 103-11, 1998 (PubMed).