DMC1 Dosage Suppressor of Mck1 Homolog, Meiosis-Specific Homologous Recombination (Yeast) (DMC1) antibody

Details for Product No. ABIN250518
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Antigen
Synonyms DMC1, zgc:136628, Dmc1h, Lim15, Mei11, sgdp, DCM1, DMC1H, LIM15, dJ199H16.1
Reactivity
Human
(56), (20), (16), (12), (12), (12)
Host
Mouse
(45), (12)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunoprecipitation (IP)
(42), (21), (21), (11), (10), (7), (3), (2), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 0.025 mL
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Catalog No. ABIN250518
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Immunogen His-tagged human DMC1, overexpressed in E. coli
Clone 1D12-4
Isotype IgG1
Cross-Reactivity (Details) Does not cross-react with human Rad51 in Western analysis.
Purification Ascites
Alternative Name DMC1 / LIM15
Background DMC-1 is a meiosis-specific homologue of RecA/Rad51 and is an essential componentof the meiotic recombination machinery in yeast and higher eukaryotes. DMC-1 is thehuman equivalent of e. coli recA. Alternate Names: anti-disrupted meiotic cDNA 1 homolog antibody, anti-dJ199H16.1 antibody, anti-DMC 1antibody, anti-DMC1 homologue antibody, anti-DMC1H antibody, anti-HsLim15antibody, anti-Meiotic recombination protein DMC1/LIM15 homolog antibody.
Gene Symbol: DMC1
Gene ID 11144
UniProt Q14565
Application Notes This DMC1 (1D12/4) antibody is useful for Western Blot, where a band can be seen at ~37 kDa for endogenous protein and ~40.5 kDa for recombinant his-tagged DMC1. Immunoprecipitation was reported in scientific literature.
Recommended dilutions: Immunoprecipitation, Western Blot 1:1000
Protocol Protocol specific for DMC1 Antibody Western BlotProcedure Guide for NB 100-181 Anti-hDMC-1Western Blot
1. Proteins are separated on an SDS-PAGE gel.
. Following the protein transfer, the membrane is blocked with 5 % NFDM in TBS-T.
. The membrane is then washed.
. NB 100-181 [anti-hDMC] is diluted 1:1,000 in dilution buffer [5 % NFDM in TBS-T] and incubated for 3 hours at room temperature, gently shaking.
. The membrane is then washed.
. Secondary antibody [HRP conjugated goat anti-mouse] is incubated for 45 minutes at room temperature, gently shaking.
. Develop membrane.NOTE: Mouse testis tissue extracts were used as a positive control for this antibody. MEF 161 and HeLa whole cell extracts were not shown to work in Western analysis.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
General Masson, Davies, Hajibagheri et al.: "The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51." in: The EMBO journal, Vol. 18, Issue 22, pp. 6552-60, 2000 (PubMed).

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