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FANCD2 antibody (Fanconi Anemia, Complementation Group D2) (N-Term) (HRP)

Details for Product anti-FANCD2 Antibody No. ABIN250521, Supplier: Log in to see
Antigen
  • 2410150O07Rik
  • AU015151
  • BB137857
  • CG17269
  • CG31192
  • CG31194
  • Dmel\\CG17269
  • dmFANCD2
  • FA-D2
  • FA4
  • FACD
  • FAD
  • FAD2
  • FANCD
  • FANCD2
  • xfancd2
Epitope
N-Term
16
13
10
5
4
4
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
99
47
38
3
2
Host
Rabbit
91
13
Clonality
Polyclonal
Conjugate
This FANCD2 antibody is conjugated to HRP
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
67
39
28
23
21
13
9
2
2
1
Supplier
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Immunogen Human FANCD2 fusion protein (N-terminal fragment).
Specificity This antibody is specific for FANCD2.
Purification Affinity purified
Alternative Name FANCD2 (FANCD2 Antibody Abstract)
Background Fanconi anemia (FANC) is a human autosomal-recessive cancer susceptibility disordercharacterized by congenital defects, progressive bone marrow failure, and cellularhypersensitivity to mitomycin C (MMC). The FANC subunit D2 protein is vital for cellularresistance to DNA cross-linking and the arrest of DNA synthesis after ionizing radiation. DNA damage activates the monoubiquitination of FANCD2, targeting nuclear focicontaining the BRCA1 protein. Alternate Names: anti-FA4 antibody, anti-FACD antibody, anti-FAD antibody, anti-FAD2 antibody,anti-FANCD antibody, anti-Fanconi Anemia Complementation Group D antibody,anti-Fanconi Pancytopenia Type 4 antibody.
Gene Symbol: FANCD2
Gene ID 2177, 211651
UniProt Q9BXW9
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes By Western blot, this antibody should recognize a band at ~166 kDa (post-translationally modified form). Additional bands may be seen at lower molecular weights. For immunofluorescence it has been tested in human MMC and IR treated MEF cells. This antibody may also be used for Immunoprecipitation and Immunohistochemistry-Paraffin
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:200-1:500, Immunohistochemistry 2.5-5.0 µg/mL, Immunohistochemistry-Paraffin 2.5-5.0 µg/mL, Immunoprecipitation 1:10-1:500, Western Blot 1:10000-1:20000
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Preservative Without preservative
Storage 4 °C
Storage Comment 4 °C in the dark
Supplier Images
Immunofluorescence (IF) image for anti-FANCD2 antibody (Fanconi Anemia, Complementation Group D2) (N-Term) (HRP) (ABIN250521) FancD2 colocalizes in vivo with another protein in SiHa cells after cell exposure to ...
Immunofluorescence (IF) image for anti-FANCD2 antibody (Fanconi Anemia, Complementation Group D2) (N-Term) (HRP) (ABIN250521) FancD2 colocalizes in vivo with another protein in U2OS cells after cell exposure to ...
Western Blotting (WB) image for anti-FANCD2 antibody (Fanconi Anemia, Complementation Group D2) (N-Term) (HRP) (ABIN250521) FANCD2 in HeLa WCE using ABIN151329 (lot C).
Product cited in: Oda, Hayano, Miyaso, Takahashi, Yamashita: "Hsp90 regulates the Fanconi anemia DNA damage response pathway." in: Blood, Vol. 109, Issue 11, pp. 5016-26, 2007

Jacquemont, Taniguchi: "Proteasome function is required for DNA damage response and fanconi anemia pathway activation." in: Cancer research, Vol. 67, Issue 15, pp. 7395-405, 2007

Bekker-Jensen, Lukas, Kitagawa, Melander, Kastan, Bartek, Lukas: "Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks." in: The Journal of cell biology, Vol. 173, Issue 2, pp. 195-206, 2006

Mukhopadhyay, Leung, Hicks, Hastings, Youssoufian, Plon: "Defective mitochondrial peroxiredoxin-3 results in sensitivity to oxidative stress in Fanconi anemia." in: The Journal of cell biology, Vol. 175, Issue 2, pp. 225-35, 2006

Dunn, Potter, Rees, Ruenger: "Activation of the Fanconi anemia/BRCA pathway and recombination repair in the cellular response to solar ultraviolet light." in: Cancer research, Vol. 66, Issue 23, pp. 11140-7, 2006

Donahue, Campbell: "A Rad50-dependent pathway of DNA repair is deficient in Fanconi anemia fibroblasts." in: Nucleic acids research, Vol. 32, Issue 10, pp. 3248-57, 2004

Rothfuss, Grompe: "Repair kinetics of genomic interstrand DNA cross-links: evidence for DNA double-strand break-dependent activation of the Fanconi anemia/BRCA pathway." in: Molecular and cellular biology, Vol. 24, Issue 1, pp. 123-34, 2003

Taniguchi, Tischkowitz, Ameziane, Hodgson, Mathew, Joenje, Mok, DAndrea: "Disruption of the Fanconi anemia-BRCA pathway in cisplatin-sensitive ovarian tumors." in: Nature medicine, Vol. 9, Issue 5, pp. 568-74, 2003

Digweed, Rothe, Demuth, Scholz, Schindler, Stumm, Grompe, Jordan, Sperling: "Attenuation of the formation of DNA-repair foci containing RAD51 in Fanconi anaemia." in: Carcinogenesis, Vol. 23, Issue 7, pp. 1121-6, 2002

Taniguchi, Garcia-Higuera, Andreassen, Gregory, Grompe, DAndrea: "S-phase-specific interaction of the Fanconi anemia protein, FANCD2, with BRCA1 and RAD51." in: Blood, Vol. 100, Issue 7, pp. 2414-20, 2002

Background publications Sridharan, Brown, Lambert, McMahon, Lambert: "Nonerythroid alphaII spectrin is required for recruitment of FANCA and XPF to nuclear foci induced by DNA interstrand cross-links." in: Journal of cell science, Vol. 116, Issue Pt 5, pp. 823-35, 2003

Lensch, Tischkowitz, Christianson, Reifsteck, Speckhart, Jakobs, ODwyer, Olson, Le Beau, Hodgson, Mathew, Larson, Bagby: "Acquired FANCA dysfunction and cytogenetic instability in adult acute myelogenous leukemia." in: Blood, Vol. 102, Issue 1, pp. 7-16, 2003

Olopade, Wei: "FANCF methylation contributes to chemoselectivity in ovarian cancer." in: Cancer cell, Vol. 3, Issue 5, pp. 417-20, 2003

Garcia-Higuera, Taniguchi, Ganesan, Meyn, Timmers, Hejna, Grompe, DAndrea: "Interaction of the Fanconi anemia proteins and BRCA1 in a common pathway." in: Molecular cell, Vol. 7, Issue 2, pp. 249-62, 2001

McMahon, Sangerman, Goodman, Kumaresan, Lambert: "Human alpha spectrin II and the FANCA, FANCC, and FANCG proteins bind to DNA containing psoralen interstrand cross-links." in: Biochemistry, Vol. 40, Issue 24, pp. 7025-34, 2001