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FANCD2 antibody (Fanconi Anemia, Complementation Group D2) (N-Term)

Details for Product anti-FANCD2 Antibody No. ABIN250523, Supplier: Log in to see
Antigen
  • 2410150O07Rik
  • AU015151
  • BB137857
  • CG17269
  • CG31192
  • CG31194
  • Dmel\\CG17269
  • dmFANCD2
  • FA-D2
  • FA4
  • FACD
  • FAD
  • FAD2
  • FANCD
  • FANCD2
  • xfancd2
Epitope
N-Term
18
16
13
5
4
4
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Monkey, Mouse (Murine)
108
55
38
8
8
3
2
Host
Rabbit
100
13
Clonality
Polyclonal
Conjugate
This FANCD2 antibody is un-conjugated
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
76
48
28
28
23
20
17
9
2
1
Supplier
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Immunogen Human FANCD2 fusion protein (N-terminal fragment).
Specificity This antibody is specific for FANCD2.
Purification Affinity purified
Alternative Name FANCD2 (FANCD2 Antibody Abstract)
Background Fanconi anemia (FANC) is a human autosomal-recessive cancer susceptibility disordercharacterized by congenital defects, progressive bone marrow failure, and cellularhypersensitivity to mitomycin C (MMC). The FANC subunit D2 protein is vital for cellularresistance to DNA cross-linking and the arrest of DNA synthesis after ionizing radiation. DNA damage activates the monoubiquitination of FANCD2, targeting nuclear focicontaining the BRCA1 protein. Alternate Names: anti-FA4 antibody, anti-FACD antibody, anti-FAD antibody, anti-FAD2 antibody,anti-FANCD antibody, anti-Fanconi Anemia Complementation Group D antibody,anti-Fanconi Pancytopenia Type 4 antibody.
Gene Symbol: FANCD2
Gene ID 2177, 211651
UniProt Q9BXW9
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes By Western blot, this antibody should recognize a band at ~166 kDa (post-translationally modified form). Additional bands may be seen at lower molecular weights. For immunofluorescence it has been tested in human MMC and IR treated MEF cells. This antibody may also be used for Immunoprecipitation and Immunohistochemistry-Paraffin
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:200-1:500, Immunohistochemistry 2.5-5.0 µg/mL, Immunohistochemistry-Paraffin 2.5-5.0 µg/mL, Immunoprecipitation 1:10-1:500, Western Blot 1:10000-1:20000
Protocol Protocol specific for FANCD2 Antibody Western Blot
1. Proteins are separated on a 7.5 % SDS-PAGE gel.
. Following the protein transfer, the membrane is blocked with TBST + 3 % milk.
. Anti-FANCD2 [cat# NB 100-182] is diluted in blocking buffer and incubated for 1 hour at room temperature, gently shaking.
. The membrane is then washed, 3 times with TBST, 5 minutes each.
. Secondary antibody is incubated for 1 hour at room temperature, gently shaking.
. The membrane is then washed, 3 times with TBST, 5 minutes each.
. Membrane is developed using ECL reagents. Visualization after a 30 second -2 minute exposure. NOTE: HeLa whole cell extracts [cat# NB 800-PC1] were used as positive control for this antibodyImmunoprecipitation 4ul for 1 mg of whole cell extract prepared by lysis in either standard RIPA buffer or a mild buffer composed of 10 mM Tris/HCl pH 8, 150 mM NaCl, 2 mM MgCl2, 0.1 % Triton X-100 + inhibitors.Immunohistochemistry - FFPE sectionsI. DeparaffinizationA. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changesB. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous PeroxidaseA. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation.B. Place slides in distilled water: 2 changes for 2 minutes each.III. Retrieve Epitopes:A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-glycine, 150 mM NaCl, pH 7.5, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
Immunofluorescence (IF) image for anti-FANCD2 antibody (Fanconi Anemia, Complementation Group D2) (N-Term) (ABIN250523) FancD2 colocalizes in vivo with another protein in SiHa cells after cell exposure to ...
Immunofluorescence (IF) image for anti-FANCD2 antibody (Fanconi Anemia, Complementation Group D2) (N-Term) (ABIN250523) FancD2 colocalizes in vivo with another protein in U2OS cells after cell exposure to ...
Western Blotting (WB) image for anti-FANCD2 antibody (Fanconi Anemia, Complementation Group D2) (N-Term) (ABIN250523) FANCD2 in HeLa WCE using ABIN151329 (lot C).
Product cited in: Panneerselvam, Wang, Zhang, Che, Yu, Fei: "BLM promotes the activation of Fanconi Anemia signaling pathway." in: Oncotarget, Vol. 7, Issue 22, pp. 32351-61, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Wang, Gao, Lu, Tatetsu, Williams, Müller, Cui, Chai: "Leukemic survival factor SALL4 contributes to defective DNA damage repair." in: Oncogene, Vol. 35, Issue 47, pp. 6087-6095, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Maeda, Froning, Brents, Rose, Thamm, Kato: "Intrinsic Radiosensitivity and Cellular Characterization of 27 Canine Cancer Cell Lines." in: PLoS ONE, Vol. 11, Issue 6, pp. e0156689, 2016 (PubMed). (Sample species: Dog (Canine)). Further details: Western Blotting

Chun, Hwang, Kim, Goh, Kim, Lee: "Aurora A kinase is required for activation of the Fanconi anemia/BRCA pathway upon DNA damage." in: FEBS open bio, Vol. 6, Issue 7, pp. 782-90, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Root, Larsen, Komosa, Al-Azri, Li, Bazett-Jones, Stephen Meyn: "FANCD2 limits BLM-dependent telomere instability in the alternative lengthening of telomeres pathway." in: Human molecular genetics, 2016 (PubMed).

Zhu, Yan, Li, Lin, Zhang, He, Zheng, Yang, Shao: "K63-linked ubiquitination of FANCG is required for its association with the Rap80-BRCA1 complex to modulate homologous recombination repair of DNA interstand crosslinks." in: Oncogene, Vol. 34, Issue 22, pp. 2867-78, 2015 (PubMed).

Duan, Gao, Aguila, Kalvala, Otterson, Villalona-Calero: "Fanconi anemia repair pathway dysfunction, a potential therapeutic target in lung cancer." in: Frontiers in oncology, Vol. 4, pp. 368, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Stoepker, Faramarz, Rooimans, van Mil, Balk, Velleuer, Ameziane, Te Riele, de Winter: "DNA helicases FANCM and DDX11 are determinants of PARP inhibitor sensitivity." in: DNA repair, Vol. 26, pp. 54-64, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Aarts, Bajrami, Herrera-Abreu, Elliott, Brough, Ashworth, Lord, Turner: "Functional Genetic Screen Identifies Increased Sensitivity to WEE1 Inhibition in Cells with Defects in Fanconi Anemia and HR Pathways." in: Molecular cancer therapeutics, Vol. 14, Issue 4, pp. 865-76, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Brem, Zhang, Xu, Karran: "UVA photoactivation of DNA containing halogenated thiopyrimidines induces cytotoxic DNA lesions." in: Journal of photochemistry and photobiology. B, Biology, Vol. 145, pp. 1-10, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Xie, Kim, Moreau, Puhalla, Garber, Al Abo, Takeda, DAndrea: "RNF4-mediated polyubiquitination regulates the Fanconi anemia/BRCA pathway." in: The Journal of clinical investigation, Vol. 125, Issue 4, pp. 1523-32, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Oka, Bekker-Jensen, Mailand: "Ubiquitin-like protein UBL5 promotes the functional integrity of the Fanconi anemia pathway." in: The EMBO journal, Vol. 34, Issue 10, pp. 1385-98, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Zhang, Wilson, Mahmood Ali, Namekawa, Andreassen, Ruhikanta Meetei, Pang: "Loss of Faap20 Causes Hematopoietic Stem and Progenitor Cell Depletion in Mice Under Genotoxic Stress." in: Stem cells (Dayton, Ohio), Vol. 33, Issue 7, pp. 2320-30, 2015 (PubMed).

Rickman, Lach, Abhyankar, Donovan, Sanborn, Kennedy, Sougnez, Gabriel, Elemento, Chandrasekharappa, Schindler, Auerbach, Smogorzewska: "Deficiency of UBE2T, the E2 Ubiquitin Ligase Necessary for FANCD2 and FANCI Ubiquitination, Causes FA-T Subtype of Fanconi Anemia." in: Cell reports, Vol. 12, Issue 1, pp. 35-41, 2015 (PubMed).

Stoepker, Ameziane, van der Lelij, Kooi, Oostra, Rooimans, van Mil, Brink, Dietrich, Balk, Ylstra, Joenje, Feller, Brakenhoff: "Defects in the Fanconi Anemia Pathway and Chromatid Cohesion in Head and Neck Cancer." in: Cancer research, Vol. 75, Issue 17, pp. 3543-53, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Bouffard, Plourde, Bélanger, Ouellette, Labrie, Durocher: "Analysis of a FANCE Splice Isoform in Regard to DNA Repair." in: Journal of molecular biology, Vol. 427, Issue 19, pp. 3056-73, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Minocherhomji, Ying, Bjerregaard, Bursomanno, Aleliunaite, Wu, Mankouri, Shen, Liu, Hickson: "Replication stress activates DNA repair synthesis in mitosis." in: Nature, Vol. 528, Issue 7581, pp. 286-90, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Huard, Tremblay, Magron, Lévesque, Carreau: "The Fanconi anemia pathway has a dual function in Dickkopf-1 transcriptional repression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 6, pp. 2152-7, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Panneerselvam, Pickering, Han, Li, Zheng, Zhang, Zhang, Fei: "Basal level of FANCD2 monoubiquitination is required for the maintenance of a sufficient number of licensed-replication origins to fire at a normal rate." in: Oncotarget, Vol. 5, Issue 5, pp. 1326-37, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Sarbajna, Davies, West: "Roles of SLX1-SLX4, MUS81-EME1, and GEN1 in avoiding genome instability and mitotic catastrophe." in: Genes & development, Vol. 28, Issue 10, pp. 1124-36, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence