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FUT4 antibody (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific))

Details for Product anti-FUT4 Antibody No. ABIN250525, Supplier: Log in to see
Antigen
  • AI451562
  • CD15
  • ELFT
  • FAL
  • FCT3A
  • FUC-TIV
  • Fuct
  • FucT-IV
  • FUTIV
  • LeX
  • SSEA-1
  • Ssea1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
492
105
65
14
3
3
1
Host
Mouse
439
72
Clonality (Clone)
Monoclonal ()
Conjugate
This FUT4 antibody is un-conjugated
64
38
31
14
13
13
11
9
9
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6
6
6
6
6
5
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
394
113
108
101
83
60
53
49
36
12
12
12
7
6
4
3
2
2
1
1
1
1
1
1
Supplier
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Immunogen F9 tetracarcinoma stem cells (X-irradiated).
Clone MC-480
Isotype IgM
Purification IgM purified
Alternative Name SSEA-1 (FUT4 Antibody Abstract)
Background Gene Symbol: FUT4
Gene ID 2526
Application Notes Western Blot 2 μg/mL, Simple Western 1:10, Flow Cytometry 1:50-1:200, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:50-1:200, Immunohistochemistry-Frozen 1:50-1:200
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol Specific for NB100-1831 Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-glycine, 150 mM NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunocytochemistry (ICC) image for anti-FUT4 antibody (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific)) (ABIN250525) anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody
Immunofluorescence (IF) image for anti-FUT4 antibody (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific)) (ABIN250525) Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (MC-480) [ABIN151341] - SSEA1...
Western Blotting (WB) image for anti-FUT4 antibody (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific)) (ABIN250525) Western Blot: SSEA-1 Antibody (MC-480) [ABIN151341] - Western blot analysis of Ntera2...
Flow Cytometry (FACS) image for anti-FUT4 antibody (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific)) (ABIN250525) Flow Cytometry: SSEA-1 Antibody (MC-480) [ABIN151341] - Staining of NTERA-2 cells usi...
Simple Western (SimWes) image for anti-FUT4 antibody (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific)) (ABIN250525) Simple Western: SSEA-1 Antibody (MC-480) [ABIN151341] - Simple Western lane view show...
Product cited in: Meng, Su, Liu, Wang, Wang: "Rac1 contributes to cerebral ischemia reperfusion-induced injury in mice by regulation of Notch2." in: Neuroscience, Vol. 306, pp. 100-14, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Matin, Walsh, Gokhale, Draper, Bahrami, Morton, Moore, Andrews: "Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells." in: Stem cells (Dayton, Ohio), Vol. 22, Issue 5, pp. 659-68, 2004 (PubMed). (Sample species: Human). Further details: Flow Cytometry

Kanatsu-Shinohara, Ogonuki, Inoue, Miki, Ogura, Toyokuni, Shinohara: "Long-term proliferation in culture and germline transmission of mouse male germline stem cells." in: Biology of reproduction, Vol. 69, Issue 2, pp. 612-6, 2003 (PubMed). (Sample species: Mouse (Murine)). Further details: Flow Cytometry

Henderson, Draper, Baillie, Fishel, Thomson, Moore, Andrews: "Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens." in: Stem cells (Dayton, Ohio), Vol. 20, Issue 4, pp. 329-37, 2002 (PubMed).

Reubinoff, Pera, Fong, Trounson, Bongso: "Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro." in: Nature biotechnology, Vol. 18, Issue 4, pp. 399-404, 2000 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence