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FUT4 antibody (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific))

Details for Product anti-FUT4 Antibody No. ABIN250525, Supplier: Log in to see
Antigen
  • AI451562
  • CD15
  • FAL
  • FucT-IV
  • LeX
  • SSEA-1
  • Ssea1
  • ELFT
  • FCT3A
  • FUC-TIV
  • FUTIV
  • Fuct
  • fucosyltransferase 4
  • fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
  • Fut4
  • FUT4
Reactivity
Human, Mouse (Murine), Rat (Rattus)
514
137
91
15
3
3
1
Host
Mouse
434
100
Clonality (Clone)
Monoclonal ()
Conjugate
This FUT4 antibody is un-conjugated
63
38
32
14
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6
5
5
5
5
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
413
140
118
102
74
69
55
50
38
13
13
12
9
7
7
6
5
3
2
2
1
1
1
1
Options
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Immunogen F9 tetracarcinoma stem cells (X-irradiated).
Clone MC-480
Isotype IgM
Purification IgM purified
Alternative Name SSEA-1 (FUT4 Antibody Abstract)
Background Gene Symbol: FUT4
Gene ID 2526
Application Notes Western Blot 2 μg/mL, Simple Western 1:10, Flow Cytometry 1:50-1:200, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:50-1:200, Immunohistochemistry-Frozen 1:50-1:200This SSEA1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen and Paraffin. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol Specific for NB100-1831 Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunocytochemistry (ICC) image for anti-FUT4 antibody (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific)) (ABIN250525) anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody
Immunofluorescence (IF) image for anti-FUT4 antibody (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific)) (ABIN250525) Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (MC-480) [ABIN151341] - SSEA1...
Western Blotting (WB) image for anti-FUT4 antibody (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific)) (ABIN250525) Western Blot: SSEA-1 Antibody (MC-480) [ABIN151341] - Western blot analysis of Ntera2...
Flow Cytometry (FACS) image for anti-FUT4 antibody (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific)) (ABIN250525) Flow Cytometry: SSEA-1 Antibody (MC-480) [ABIN151341] - Staining of NTERA-2 cells usi...
Simple Western (SimWes) image for anti-FUT4 antibody (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific)) (ABIN250525) Simple Western: SSEA-1 Antibody (MC-480) [ABIN151341] - Simple Western lane view show...
Product cited in: Meng, Su, Liu, Wang, Wang: "Rac1 contributes to cerebral ischemia reperfusion-induced injury in mice by regulation of Notch2." in: Neuroscience, Vol. 306, pp. 100-14, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Matin, Walsh, Gokhale, Draper, Bahrami, Morton, Moore, Andrews: "Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells." in: Stem cells (Dayton, Ohio), Vol. 22, Issue 5, pp. 659-68, 2004 (PubMed). (Sample species: Human). Further details: Flow Cytometry

Kanatsu-Shinohara, Ogonuki, Inoue, Miki, Ogura, Toyokuni, Shinohara: "Long-term proliferation in culture and germline transmission of mouse male germline stem cells." in: Biology of reproduction, Vol. 69, Issue 2, pp. 612-6, 2003 (PubMed). (Sample species: Mouse (Murine)). Further details: Flow Cytometry

Henderson, Draper, Baillie, Fishel, Thomson, Moore, Andrews: "Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens." in: Stem cells (Dayton, Ohio), Vol. 20, Issue 4, pp. 329-37, 2002 (PubMed).

Reubinoff, Pera, Fong, Trounson, Bongso: "Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro." in: Nature biotechnology, Vol. 18, Issue 4, pp. 399-404, 2000 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence