Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody

Details for Product No. ABIN250525
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Antigen
Synonyms AI451562, CD15, FAL, FucT-IV, LeX, SSEA-1, Ssea1, ELFT, FCT3A, FUC-TIV, FUTIV, Fuct
Reactivity
Human, Mouse (Murine)
(226), (30), (13), (1), (1)
Host
Mouse
(201), (33)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(32), (22), (9), (7), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(155), (51), (41), (35), (29), (27), (26), (20), (19), (10), (10), (9), (8), (3), (1), (1)
Pubmed 1 reference available
Quantity 0.025 mL
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Catalog No. ABIN250525
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Immunogen F9 tetracarcinoma stem cells (X-irradiated).
Clone MC-480
Isotype IgM
Specificity This antibody recognizes SSEA1 in mouse EC,ES cells but not human, but SSEA1 is expressed by some human differentiated cell types (ie: trophoblasts).
Purification IgM purified
Alternative Name CD15
Background SSEA1 (stage-specific mouse embryonic antigen) is a cell-surface protein involved incell differentiation. Cell surface antigens are powerful tools for characterizing thefunction and expression of developmentally regulated cells during both embryogenesisand oncogenesis.
Gene Symbol: FUT4
Gene ID 2526
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Application Notes This SSEA1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen and Paraffin.
Recommended dilutions: Flow Cytometry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry 1:50-1:200, Immunohistochemistry-Frozen 1:50-1:200, Immunohistochemistry-Paraffin 1:50-1:200, Western Blot 2 µg/mL
Protocol Protocol Specific for NB100-1831 Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-glycine, 150 mM NaCl, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Henderson, Draper, Baillie et al.: "Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens." in: Stem cells (Dayton, Ohio), Vol. 20, Issue 4, pp. 329-37, 2002 (PubMed).

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