Stage Specific Embryonic Antigen 3 (SSEA3) antibody

Antigen: Stage Specific Embryonic Antigen 3 (SSEA3)

Clonality: Monoclonal (MC-631)

Host: Rat

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN250526

Additional Information

Alternative name: SSEA3

Gene ID: 93961

Immunogen: 4-8 cell stage mouse embryos.

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgM

Clone: MC-631

Description: SSEA3 (stage-specific mouse embryonic antigen) is a cell-surface protein involved incell differentiation. Cell surface antigens are powerful tools for characterizing thefunction and expression of developmentally regulated cells during both embryogenesisand oncogenesis.

Specificity: Species Reactivity: This antibody reacts with the Stage-specific embryonic antigen-3 (SSEA-3) that isexpressed upon the surface of human teratocarcinoma stem cells (EC), humanembryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivityis evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-3 isdown regulated following differentiation of human EC cells. In contrast, thedifferentiation of murine EC and ES cells may be accompanied by an increase inSSEA-3 expression.

Application Details

Application Notes: This antibody may be useful for FACS, immunofluoresence, and immunohistochemistry. Other applications have not been tested.

Buffer: Ascites containing 0.1% sodium azide.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Publications

Henderson, Draper, Baillie et al.: "Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens." in: Stem cells (Dayton, Ohio), Vol. 20, Issue 4, pp. 329-37, 2002 (PubMed).