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Thiosulfate Sulfurtransferase, SSEA-3 (SSEA-3) antibody

Details for Product No. ABIN250526, Supplier: Log in to see
Antigen
Reactivity
Human, Mouse (Murine)
45
44
Host
Rat
45
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
5
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
43
19
16
16
16
16
9
8
2
2
2
1
1
1
Options
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Immunogen 4-8 cell stage mouse embryos.
Clone MC-631
Isotype IgM
Purification IgM purified
Alternative Name SSEA-3
Application Notes Western Blot 1:500, Flow Cytometry 10-20 μg/mL, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:50-1:100, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, CyTOF-readyThis SSEA3 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry frozen and paraffin sections, Immunoprecipitation and Western Blot.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol Specific for NB100-1832 Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunocytochemistry (ICC) image for anti-Thiosulfate Sulfurtransferase, SSEA-3 (SSEA-3) antibody (ABIN250526) anti-Thiosulfate Sulfurtransferase, SSEA-3 (SSEA-3) antibody
Immunofluorescence (IF) image for anti-Thiosulfate Sulfurtransferase, SSEA-3 (SSEA-3) antibody (ABIN250526) Immunocytochemistry/Immunofluorescence: SSEA-3 Antibody (MC-631) [ABIN151342] - HeLa ...
Flow Cytometry (FACS) image for anti-Thiosulfate Sulfurtransferase, SSEA-3 (SSEA-3) antibody (ABIN250526) Flow Cytometry: SSEA-3 Antibody (MC-631) [ABIN151342] - Staining of NTERA-2 cells usi...
Product cited in: Skottman: "Derivation and characterization of three new human embryonic stem cell lines in Finland." in: In vitro cellular & developmental biology. Animal, Vol. 46, Issue 3-4, pp. 206-9, 2010 (PubMed).

Hakala, Rajala, Ojala, Panula, Areva, Kellomäki, Suuronen, Skottman: "Comparison of biomaterials and extracellular matrices as a culture platform for multiple, independently derived human embryonic stem cell lines." in: Tissue engineering. Part A, Vol. 15, Issue 7, pp. 1775-85, 2009 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Matin, Walsh, Gokhale, Draper, Bahrami, Morton, Moore, Andrews: "Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells." in: Stem cells (Dayton, Ohio), Vol. 22, Issue 5, pp. 659-68, 2004 (PubMed).

Henderson, Draper, Baillie, Fishel, Thomson, Moore, Andrews: "Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens." in: Stem cells (Dayton, Ohio), Vol. 20, Issue 4, pp. 329-37, 2002 (PubMed). (Sample species: Human). Further details: Flow Cytometry

Przyborski: "Isolation of human embryonal carcinoma stem cells by immunomagnetic sorting." in: Stem cells (Dayton, Ohio), Vol. 19, Issue 6, pp. 500-4, 2001 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Kannagi, Cochran, Ishigami, Hakomori, Andrews, Knowles, Solter: "Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells." in: The EMBO journal, Vol. 2, Issue 12, pp. 2355-61, 1984 (PubMed). (Sample species: Human). Further details: Western Blotting