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Details for Product No. ABIN250635

Egl Nine Homolog 1 (C. Elegans) (EGLN1) antibody

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Synonyms C1orf12, ECYT3, HIF-PH2, HIFPH2, HPH-2, HPH2, PHD2, SM20, ZMYND6, AI503754, Hif-p4h-2, ORF13, Phd2, SM-20, zgc:92314, PHD-2, egln2, phd2, EGLN1
»Alternatives Mouse (Murine)
»Alternatives Rabbit
Clonality Polyclonal
»Alternatives Un-conjugated
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 1 reference available
Catalog no. ABIN250635
Quantity 0.1 mL
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Immunogen A synthetic peptide made to an internal portion of the mouse PHD2 protein sequence(between residues 300-400).
Specificity This antibody is specific for PHD2. Species Reactivity: This antibody reacts with mouse PHD2 protein. No other species have been tested. The immunogen used for this antibody production has 100% homology with rat protein. There is no significant homology with the human protein.
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Aff - Purified
Alternative Name EGLN1 / PHD2
Background HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolylhydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIFis an intracellular protein and the HPH sites do not resemble those modifying collagen.HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH isan essential component of the pathway through which cells sense oxygen. In thepresence of oxygen, HPHs convert specific prolyl residues in HIF-alpha tohydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at thecellular level, cause the HIF conversion to be reduced so that HIF is stable and there isincreased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIFalpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. Ittargets HIF through the hydroxylation for proteasomal degradation via the vonHippel-Lindau ubiquitylation complex. Alternate Names: anti-PHD2 antibody, anti-C1ORF12 antibody, anti-Chromosome 1 Open Reading Frame12 antibody, anti-EGL 9 homolog of C. elegans 1 antibody, anti-EGLN 1 EGLN1antibody, anti-HIF Prolyl Hydroxylase 2 antibody, anti-HIF-P4H 2 antibody, anti-HIFPH2antibody, anti-P4H2 antibody, anti-Prolyl Hydroxylase Domain Containing antibody,anti-PHD2 antibody, anti-EGLN1 antibody.
Gene ID 54583, 112405
UniProt Q91YE3
Application Notes Suggested working dilutions: immunohistochemistry 2.5ug/ml,Western Blot 2 ug/ml. Positive Controls: Mouse kidney lysate
Restrictions For Research Use only
Concentration 1 mg/ml
Buffer Tris-citrate/phosphate [pH 7-8]. Preservative: 0.1% sodium azide.
Preservative Sodium azide
Storage 4 °C
Mikhaylova, Ignacak, Barankiewicz et al.: "The von Hippel-Lindau tumor suppressor protein and Egl-9-Type proline hydroxylases regulate the large subunit of RNA polymerase II in response to oxidative stress." in: Molecular and cellular biology, Vol. 28, Issue 8, pp. 2701-17, 2008 (PubMed).

Alternatives for antigen "Egl Nine Homolog 1 (C. Elegans) (EGLN1)", type "Antibodies"
Hosts (56), (22)
Reactivities (71), (30), (12), (12), (6), (1), (1), (1)
Applications (65), (16), (14), (14), (13), (10), (10), (7), (3), (3)
Conjugates (7), (5), (5), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (13), (10), (6), (5), (5), (3), (2), (2), (1), (1), (1), (1)