Egl Nine Homolog 1 (C. Elegans) (EGLN1) antibody

Antigen: Egl Nine Homolog 1 (C. Elegans) (EGLN1)

Synonyms: HPH2, PHD2, SM20, ECYT3, HIFPH2, ZMYND6, C1orf12, DKFZp761F179, Phd2, ORF13, SM-20, AI503754, zgc:92314, HPH-2, PHD-2, HIF-PH2, EGLN1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN250635

Additional Information

Alternative name: EGLN1 / PHD2

Gene ID: 54583, 112405

UniProt: Q91YE3

Immunogen: A synthetic peptide made to an internal portion of the mouse PHD2 protein sequence(between residues 300-400).

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Description: HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolylhydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIFis an intracellular protein and the HPH sites do not resemble those modifying collagen.HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH isan essential component of the pathway through which cells sense oxygen. In thepresence of oxygen, HPHs convert specific prolyl residues in HIF-alpha tohydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at thecellular level, cause the HIF conversion to be reduced so that HIF is stable and there isincreased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIFalpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. Ittargets HIF through the hydroxylation for proteasomal degradation via the vonHippel-Lindau ubiquitylation complex. Alternate Names: anti-PHD2 antibody, anti-C1ORF12 antibody, anti-Chromosome 1 Open Reading Frame12 antibody, anti-EGL 9 homolog of C. elegans 1 antibody, anti-EGLN 1 EGLN1antibody, anti-HIF Prolyl Hydroxylase 2 antibody, anti-HIF-P4H 2 antibody, anti-HIFPH2antibody, anti-P4H2 antibody, anti-Prolyl Hydroxylase Domain Containing antibody,anti-PHD2 antibody, anti-EGLN1 antibody.

Specificity: This antibody is specific for PHD2. Species Reactivity: This antibody reacts with mouse PHD2 protein. No other species have been tested. The immunogen used for this antibody production has 100% homology with rat protein. There is no significant homology with the human protein.

Application Details

Application Notes: Suggested working dilutions: immunohistochemistry 2.5ug/ml,Western Blot 2 ug/ml. Positive Controls: Mouse kidney lysate

Concentration: 1 mg/ml

Purification: Aff - Purified

Buffer: Tris-citrate/phosphate [pH 7-8]. Preservative: 0.1% sodium azide.

Storage: Store at 4C. Do not freeze.

Restrictions: For Research Use only

Images

Immunohistochemistry: HIF Prolyl Hydroxylase 2 Antibody [ABIN250635] - Staining of renal tubular epithelium in mouse using ABIN250635 at 2.5ug/ml. Mouse kidney cortex, 40X.

Detection of PHD2 in mouse kidney lysate using ABIN250635. ECL exposure, 20 seconds.

Publications

Mikhaylova, Ignacak, Barankiewicz et al.: "The von Hippel-Lindau tumor suppressor protein and Egl-9-Type proline hydroxylases regulate the large subunit of RNA polymerase II in response to oxidative stress." in: Molecular and cellular biology, Vol. 28, Issue 8, pp. 2701-17, 2008 (PubMed).