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Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 300-400), (Internal Region) antibody

Details for Product No. ABIN250637, Supplier: Log in to see
Antigen
  • EGLN1
  • egln2
  • phd2
  • zgc:92314
  • AI503754
  • C1orf12
  • HIF-PH2
  • HPH-2
  • Hif-p4h-2
  • ORF13
  • Phd2
  • SM-20
  • PHD-2
  • PHD2
  • ECYT3
  • HIFPH2
  • HPH2
  • SM20
  • ZMYND6
  • egl nine homolog 1 (C. elegans)
  • egl nine homolog 1
  • EGL nine homolog 1 (C. elegans)
  • egl-9 family hypoxia-inducible factor 1
  • EGLN1
  • egln1
  • Egln1
Alternatives
anti-Human Egl Nine Homolog 1 (C. Elegans) antibody for Flow Cytometry
Epitope
AA 300-400, Internal Region
38
32
18
15
13
8
8
7
5
5
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine)
151
63
42
13
3
2
1
1
1
1
1
1
1
1
Host
Rabbit
119
37
2
Clonality
Polyclonal
Conjugate
Un-conjugated
13
10
8
8
8
7
5
5
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
129
80
60
49
35
35
28
13
12
2
2
2
1
1
1
1
Options
Supplier
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Immunogen A synthetic peptide made to an internal portion of mouse PHD2/HIF Prolyl Hydroxylase 2 (between residues 300-400). [UniProt# Q91YE3]
Purification Immunogen affinity purified
Alternative Name EGLN1/PHD2 (EGLN1 Antibody Abstract)
Background Gene Symbol: EGLN1
Molecular Weight Theoretical MW: 43 kDa
Gene ID 54583
UniProt Q91YE3
Pathways cAMP Metabolic Process
Application Notes Western Blot 2 μg/mL, Immunohistochemistry 2.5 μg/mL, Immunocytochemistry/Immunofluorescence 1:50-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 2.5 μg/mLThis HIF Prolyl Hydroxylase 2 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin embedded sections and Immunoprecipitation. In Western blot a band is seen ~43 kDa representing HIF Prolyl Hydroxylase 2. There is also a non-specific band of similar intensity at ~75 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for HIF Prolyl Hydroxylase 2 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 2 hours at room temperature (RT).
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-PHD2 (murine) primary antibody in blocking buffer and incubate 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at RT.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (we used BioFX Super Plus ECL). Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding. IHC-FFPE sectionsI. Deparaffinization: Western Blot Protocol
. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 2 hours at room temperature (RT).
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Immunohistochemistry (IHC) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 300-400), (Internal Region) antibody (ABIN250637) Immunohistochemistry: HIF Prolyl Hydroxylase 2 Antibody [ABIN250635] - Staining of re...
Western Blotting (WB) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 300-400), (Internal Region) antibody (ABIN250637) Detection of PHD2 in mouse kidney lysate using ABIN250635. ECL exposure, 20 seconds.
Immunofluorescence (IF) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 300-400), (Internal Region) antibody (ABIN250637) Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody [ABIN250635] - Analysis o...
Immunofluorescence (IF) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 300-400), (Internal Region) antibody (ABIN250637) Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody [ABIN250635] - EGLN1/PHD2...
Product cited in: Choi, Kim, Song, Suh, Kim, Kim, Na, Nadas, Dong, Cha, Surh: "15-Deoxy-Δ(12,14)-prostaglandin J2 stabilizes hypoxia inducible factor-1α through induction of heme oxygenase-1 and direct modification ofprolyl-4-hydroxylase 2." in: Free radical research, Vol. 50, Issue 10, pp. 1140-1152, 2016 (PubMed). (Sample species: Human). Further details: Immunoprecipitation

Karsikas, Myllymäki, Heikkilä, Sormunen, Kivirikko, Myllyharju, Serpi, Koivunen: "HIF-P4H-2 deficiency protects against skeletal muscle ischemia-reperfusion injury." in: Journal of molecular medicine (Berlin, Germany), Vol. 94, Issue 3, pp. 301-10, 2016 (PubMed).

Zeng, He, Tuo, Liao, Zhang, Chen: "LPS causes pericyte loss and microvascular dysfunction via disruption of Sirt3/angiopoietins/Tie-2 and HIF-2α/Notch3 pathways." in: Scientific reports, Vol. 6, pp. 20931, 2016 (PubMed). Further details: Western Blotting

Wu, Zhang, Huang, Jia, Wang, Xu, Yang, Miyagishi, Kasim: "Prolyl hydroxylase domain-2 silencing induced by hydrodynamic limb vein injection enhances vascular regeneration in critical limb ischemia mice through activation of multiple genes." in: Current gene therapy, Vol. 15, Issue 3, pp. 313-25, 2015 (PubMed). (Sample species: Mouse (Murine)).

Xie, Pi, Townley-Tilson, Li, Wehrens, Entman, Taffet, Mishra, Peng, Schisler, Meissner, Patterson: "PHD2/3-dependent hydroxylation tunes cardiac response to β-adrenergic stress via phospholamban." in: The Journal of clinical investigation, Vol. 125, Issue 7, pp. 2759-71, 2015 (PubMed). Further details: Western Blotting

Kim, Kwak, Cha, Jeong, Rhee, Cheon: "The role of prolyl hydroxylase domain protein (PHD) during rosiglitazone-induced adipocyte differentiation." in: The Journal of biological chemistry, Vol. 289, Issue 5, pp. 2755-64, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

An, Xu, Yang, Wang, Li, McHepange, Shen, Tu, Tao et al.: "Expression of hypoxia-inducible factor-1α, vascular endothelial growth factor and prolyl hydroxylase domain protein 2 in cutaneous squamous cell carcinoma and precursor lesions and their relationship ..." in: The Journal of dermatology, Vol. 41, Issue 1, pp. 76-83, 2014 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Swain, Wottawa, Hillemann, Beneke, Odagiri, Terada, Endo, Oike, Farhat, Katschinski: "Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions." in: Journal of leukocyte biology, Vol. 96, Issue 3, pp. 365-75, 2014 (PubMed). (Sample species: Mouse (Murine)).

Hesse, Levent, Zieseniss, Tiburcy, Zimmermann, Katschinski: "Lights on for HIF-1α: genetically enhanced mouse cardiomyocytes for heart tissue imaging." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 34, Issue 2, pp. 455-62, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Pisarcik, Maylor, Lu, Yun, Undem, Sylvester, Semenza, Shimoda: "Activation of hypoxia-inducible factor-1 in pulmonary arterial smooth muscle cells by endothelin-1." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 304, Issue 8, pp. L549-61, 2013 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Palomäki, Pietilä, Laitinen, Pesälä, Sormunen, Lehenkari, Koivunen: "HIF-1α is upregulated in human mesenchymal stem cells." in: Stem cells (Dayton, Ohio), Vol. 31, Issue 9, pp. 1902-9, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Kuzmanov, Wielockx, Rezaei, Kettelhake, Breier: "Overexpression of factor inhibiting HIF-1 enhances vessel maturation and tumor growth via platelet-derived growth factor-C." in: International journal of cancer, Vol. 131, Issue 5, pp. E603-13, 2012 (PubMed).

Kunze, Zhou, Veltkamp, Wielockx, Breier, Marti: "Neuron-specific prolyl-4-hydroxylase domain 2 knockout reduces brain injury after transient cerebral ischemia." in: Stroke, Vol. 43, Issue 10, pp. 2748-56, 2012 (PubMed). (Sample species: Mouse (Murine)).

Hölscher, Silter, Krull, von Ahlen, Hesse, Schwartz, Wielockx, Breier, Katschinski, Zieseniss: "Cardiomyocyte-specific prolyl-4-hydroxylase domain 2 knock out protects from acute myocardial ischemic injury." in: The Journal of biological chemistry, Vol. 286, Issue 13, pp. 11185-94, 2011 (PubMed).

Rabie, Kunze, Marti: "Impaired hypoxic response in senescent mouse brain." in: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, Vol. 29, Issue 6, pp. 655-61, 2011 (PubMed).

Tandon, SenGupta, Ndhlovu, Vieira, Jones, York, Vieira, Sharp, Wiznia, Ostrowski, Rosenberg, Nixon: "Identification of human endogenous retrovirus-specific T cell responses in vertically HIV-1-infected subjects." in: Journal of virology, Vol. 85, Issue 21, pp. 11526-31, 2011 (PubMed). Further details: Western Blotting

Hyvärinen, Hassinen, Sormunen, Mäki, Kivirikko, Koivunen, Myllyharju: "Hearts of hypoxia-inducible factor prolyl 4-hydroxylase-2 hypomorphic mice show protection against acute ischemia-reperfusion injury." in: The Journal of biological chemistry, Vol. 285, Issue 18, pp. 13646-57, 2010 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Schödel, Klanke, Weidemann, Buchholz, Bernhardt, Bertog, Amann, Korbmacher, Wiesener, Warnecke, Kurtz, Eckardt, Willam: "HIF-prolyl hydroxylases in the rat kidney: physiologic expression patterns and regulation in acute kidney injury." in: The American journal of pathology, Vol. 174, Issue 5, pp. 1663-74, 2009 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Lehmann, Stiehl, Honer, Dominietto, Keist, Kotevic, Wollenick, Ametamey, Wenger, Rudin: "Longitudinal and multimodal in vivo imaging of tumor hypoxia and its downstream molecular events." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 33, pp. 14004-9, 2009 (PubMed).

Mikhaylova, Ignacak, Barankiewicz, Harbaugh, Yi, Maxwell, Schneider, Van Geyte, Carmeliet, Revelo, Wyder, Greis, Meller, Czyzyk-Krzeska: "The von Hippel-Lindau tumor suppressor protein and Egl-9-Type proline hydroxylases regulate the large subunit of RNA polymerase II in response to oxidative stress." in: Molecular and cellular biology, Vol. 28, Issue 8, pp. 2701-17, 2008 (PubMed). (Sample species: Human). Further details: Western Blotting