Egl Nine Homolog 2 (C. Elegans) (EGLN2) antibody

Details for Product No. ABIN250637
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Antigen
Synonyms EIT6, HIF-PH1, HIFPH1, HPH-1, HPH-3, PHD1, 0610011A13Rik, C85656, Hif-p4h-1, Ier4, Phd1, SM-20, PHD-1, egln2, phd2
Reactivity
Mouse (Murine), Rat (Rattus)
(46), (25), (22), (13), (3), (1), (1), (1)
Host
Rabbit
(44), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(37), (10), (10), (6), (1), (1), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN250637
Quantity 25 µL
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Immunogen A synthetic peptide made to an internal portion of the mouse PHD2 protein sequence(between residues 300-400).
Specificity This antibody is specific for PHD2. Species Reactivity: This antibody reacts with mouse PHD2 protein. No other species have been tested. The immunogen used for this antibody production has 100% homology with rat protein. There is no significant homology with the human protein. Localization: Endoplasmic reticulum.
Cross-Reactivity Mouse (Murine)
Purification Affinity Purified
Alternative Name EGLN2 / PHD1
Background HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolylhydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIFis an intracellular protein and the HPH sites do not resemble those modifying collagen.HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH isan essential component of the pathway through which cells sense oxygen. In thepresence of oxygen, HPHs convert specific prolyl residues in HIF-alpha tohydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at thecellular level, cause the HIF conversion to be reduced so that HIF is stable and there isincreased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIFalpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. Ittargets HIF through the hydroxylation for proteasomal degradation via the vonHippel-Lindau ubiquitylation complex. Alternate Names: anti-PHD2 antibody, anti-C1ORF12 antibody, anti-Chromosome 1 Open Reading Frame12 antibody, anti-EGL 9 homolog of C. elegans 1 antibody, anti-EGLN 1 EGLN1antibody, anti-HIF Prolyl Hydroxylase 2 antibody, anti-HIF-P4H 2 antibody, anti-HIFPH2antibody, anti-P4H2 antibody, anti-Prolyl Hydroxylase Domain Containing antibody,anti-PHD2 antibody, anti-EGLN1 antibody.
Gene ID 112406
Application Notes Suggested working dilutions: immunohistochemistry 2.5ug/ml,Western Blot 2 ug/ml. Positive Controls: Mouse kidney lysate
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/ml
Buffer Tris-citrate/phosphate [pH 7-8]. Preservative: 0.1% sodium azide.
Preservative Sodium azide
Storage 4 °C
Supplier Images
anti-Egl Nine Homolog 2 (C. Elegans) (EGLN2) antibody Immunohistochemistry: HIF Prolyl Hydroxylase 2 Antibody [ABIN250635SS] - Staining of renal tubular epithelium in mouse using ABIN250635 at 2.5ug/ml. Mouse kidney cortex, 40X.
anti-Egl Nine Homolog 2 (C. Elegans) (EGLN2) antibody (2) Western Blot: PHD2 Antibody [ABIN250635SS] - Detection of PHD2 in mouse kidney lysate using ABIN250635. ECL exposure, 20 seconds.
General Mikhaylova, Ignacak, Barankiewicz et al.: "The von Hippel-Lindau tumor suppressor protein and Egl-9-Type proline hydroxylases regulate the large subunit of RNA polymerase II in response to oxidative stress." in: Molecular and cellular biology, Vol. 28, Issue 8, pp. 2701-17, 2008 (PubMed).

Hosts (44), (2), (1)
Reactivities (46), (25), (22), (13), (3), (1), (1), (1)
Applications (37), (10), (10), (6), (1), (1), (1), (1)
Conjugates (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (8), (7), (5), (2), (2), (2), (1), (1), (1)
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