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Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody

Details for Product No. ABIN250638, Supplier: Log in to see
Antigen
  • Map1lc3
  • zgc:56434
  • wu:fb60g11
  • map1lc3b
  • MGC76283
  • MAP1LC3B
  • ATG8F
  • LC3B
  • MAP1A/1BLC3
  • MAP1LC3B-a
  • 1010001C15Rik
  • Atg8
  • LC3b
  • Mpl3
  • zbs559
  • microtubule-associated protein 1 light chain 3 beta
  • map1lc3b
  • MAP1LC3B
  • LOC100361973
  • Map1lc3b
Alternatives
anti-Cow (Bovine) Microtubule-Associated Protein 1 Light Chain 3 beta antibody for Electron Microscopy
Epitope
N-Term, AA 1-100
69
34
23
21
21
17
10
10
9
8
8
6
6
5
5
5
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Bacteria, Cow (Bovine), Dog (Canine), Golden Syrian Hamster, Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus), Zebrafish
252
141
93
31
26
26
25
24
11
11
6
6
1
Host
Rabbit
235
20
1
Clonality
Polyclonal
Conjugate
Un-conjugated
17
15
14
13
13
13
5
4
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Simple Western (SimWes), Western Blotting (WB)
144
120
76
75
68
61
57
38
25
10
8
6
3
3
2
1
1
Options
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Immunogen A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1-100). [UniProt# Q9GZQ8]
Purification Immunogen affinity purified
Alternative Name LC3B/MAP1LC3B (MAP1LC3B Antibody Abstract)
Background Gene Symbol: MAP1LC3B
Gene ID 81631
UniProt Q9GZQ8
Research Area Cytoskeleton, Autophagy, Cancer
Pathways Autophagy
Application Notes Western Blot 0.5-2 μg/mL, Simple Western 1:50, ELISA, Immunohistochemistry 1:200-1:400, Immunocytochemistry/Immunofluorescence 1:200, Immunoprecipitation 20 μg/500 μg of protein, Immunohistochemistry-Paraffin 1:200-1:400, Immunohistochemistry-Frozen, Proximity Ligation AssayThis LC3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, Immunoprecipitation and Western Blot. Use in Immunohistochemistry-Frozen sections reported in scientific literature (PMID: 20008275). Use in ELISA reported in scientific literature (PMID 20503249). In Western blot bands are seen ~17 and 19 kDa corresponding to LC3-II and LC3-I. In some cases a non-specific band is seen at ~21 kDa in mouse protein. In ICC/IF, cytoplasmic staining was observed in HeLa cells. Use in Proximity Ligation Assay reported in scientific literature (PMID 27219062). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot Protocol protocol specific for LC3 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 1 hour at room temperature (RT).
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-LC3 primary antibody in blocking buffer and incubate 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (we used BioFX Super Plus ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.I. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. II. Quench Endogenous Peroxidase:To Prepare 200 mL of Quenching Solution: Hydrogen Peroxide to 200 mL of Methanol.**Use within 4 hours of preparationA. Place slides in peroxidase quenching solution: 15-30 minutes.B. Place slides in distilled water: 2 changes for 2 minutes each. III. Retrieve Epitopes: A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250638) Western Blot: LC3 Antibody [ABIN250638SS] - Detection of LC3 in human brain lysate us...
Immunohistochemistry (IHC) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250638) Imnmunohistochemistry: LC3 Antibody [ABIN250638SS] - Staining of brain, cerebral cort...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250638) Detection of LC3 in human brain lysate using ABIN250638. Atg5-/- ES cells from Dr. No...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250638) Immunocytochemistry/Immunofluorescence: LC3B/MAP1LC3B Antibody [ABIN250638] - Analysi...
Simple Western (SimWes) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250638) Simple Western: LC3B/MAP1LC3B Antibody [ABIN250638] - Simple Western lane view shows ...
Immunohistochemistry (IHC) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250638) Immunohistochemistry: LC3B/MAP1LC3B Antibody [ABIN250638] - IHC of rat brain from con...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250638) Western Blot: LC3B/MAP1LC3B Antibody [ABIN250638] - Analysis of LC3 in H9C2 cells.
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250638) Western Blot: LC3B/MAP1LC3B Antibody [ABIN250638] - Analysis using the Biotin conjuga...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250638) Western Blot: LC3B/MAP1LC3B Antibody [ABIN250638] - Detection of LC3 in mouse HL-1 ce...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250638) Western Blot: LC3B/MAP1LC3B Antibody [ABIN250638] - Western blot analysis of HeLa (1)...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250638) Immunohistochemistry-Paraffin: LC3B/MAP1LC3B Antibody [ABIN250638] - Human ovarian Ca...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250638) Western Blot: LC3B/MAP1LC3B Antibody [ABIN250638] - analysis of LC3 in Huh-7 and SMMC...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250638) Western Blot: LC3B/MAP1LC3B Antibody [ABIN250638] - Analysis using the DyLight 550 co...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250638) Immunocytochemistry/Immunofluorescence: LC3B/MAP1LC3B Antibody [ABIN250638] - LC3 ant...
Product cited in: Mulcahy Levy, Zahedi, Griesinger, Morin, Davies, Aisner, Kleinschmidt-DeMasters, Fitzwalter, Goodall, Thorburn, Amani, Donson, Birks, Mirsky, Hankinson, Handler, Green, Vibhakar, Foreman, Thorburn: "Autophagy inhibition overcomes multiple mechanisms of resistance to BRAF inhibition in brain tumors." in: eLife, Vol. 6, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

De Luca, Carpanese, Rapanotti, Viguria, Forgione, Rotili, Fulci, Iorio, Quintieri, Chimenti, Bianchi, Rosato, Caccuri: "The nitrobenzoxadiazole derivative MC3181 blocks melanoma invasion and metastasis." in: Oncotarget, Vol. 8, Issue 9, pp. 15520-15538, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Kong, Zhu, Geng, Xia, Sun, Chen, Li, Zhou, Zhan, Xu: "The microRNA-423-3p-Bim Axis Promotes Cancer Progression and Activates Oncogenic Autophagy in Gastric Cancer." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 25, Issue 4, pp. 1027-1037, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Matos, Gouveia, Almeida: "Resveratrol Attenuates Copper-Induced Senescence by Improving Cellular Proteostasis." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 3793817, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Xue, Zeng, Chen, Chen, Tan, Zhao, Dong, Sun, Dong, Liu: "Sestrin 1 ameliorates cardiac hypertrophy via autophagy activation." in: Journal of cellular and molecular medicine, Vol. 21, Issue 6, pp. 1193-1205, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Rocchi, He: "Activating Autophagy by Aerobic Exercise in Mice." in: Journal of visualized experiments : JoVE, Issue 120, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Song, Mitter, Qi, Beli, Rao, Ding, Ip, Gu, Akin, Dunn, Bowes Rickman, Lewin, Grant, Boulton: "Oxidative stress-mediated NFκB phosphorylation upregulates p62/SQSTM1 and promotes retinal pigmented epithelial cell survival through increased autophagy." in: PLoS ONE, Vol. 12, Issue 2, pp. e0171940, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Zhang, Eitan, Mattson: "Early involvement of lysosome dysfunction in the degeneration of cerebral cortical neurons caused by the lipid peroxidation product 4-hydroxynonenal." in: Journal of neurochemistry, Vol. 140, Issue 6, pp. 941-954, 2017 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Rat (Rattus)).

Huang, Ao, Li, Wu, Xu, Deng, Chen, Yin, Cheng: "Autophagy Protects Advanced Glycation End Product-Induced Apoptosis and Expression of MMP-3 and MMP-13 in Rat Chondrocytes." in: BioMed research international, Vol. 2017, pp. 6341919, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Granato, Rizzello, Gilardini Montani, Cuomo, Vitillo, Santarelli, Gonnella, DOrazi, Faggioni, Cirone: "Quercetin induces apoptosis and autophagy in primary effusion lymphoma cells by inhibiting PI3K/AKT/mTOR and STAT3 signaling pathways." in: The Journal of nutritional biochemistry, Vol. 41, pp. 124-136, 2017 (PubMed).

Gilardini Montani, Granato, Santoni, Del Porto, Merendino, DOrazi, Faggioni, Cirone: "Histone deacetylase inhibitors VPA and TSA induce apoptosis and autophagy in pancreatic cancer cells." in: Cellular oncology (Dordrecht), Vol. 40, Issue 2, pp. 167-180, 2017 (PubMed).

Sathyanarayan: "A Coupled Approach Utilizing Immunohistochemistry and Immunocytochemistry to Visualize Cellular Lipophagy." in: Methods in molecular biology (Clifton, N.J.), Vol. 1554, pp. 185-191, 2017 (PubMed).

Delaney, Patel, Willis, Haghighiabyaneh, Axelrod, Tancioni, Lu, Bapat, Young, Cadassou, Bartakova, Sheth, Haft, Hui, Saenz, Schlaepfer, Harismendy, Stupack: "Haploinsufficiency networks identify targetable patterns of allelic deficiency in low mutation ovarian cancer." in: Nature communications, Vol. 8, pp. 14423, 2017 (PubMed).

André, Braatz, Liu, Zeitlin: "Generation and Characterization of Knock-in Mouse Models Expressing Versions of Huntingtin with Either an N17 or a Combined PolyQ and Proline-Rich Region Deletion." in: Journal of Huntington's disease, Vol. 6, Issue 1, pp. 47-62, 2017 (PubMed).

Zhu, Zhang, Zhang, Ma, Man, Liu, Zhou, Lin, Wei, Jin, Zhang, Hu, Gu, Lu, Yang, Liu, Bai, Wen: "Inhibition of Kupffer Cell Autophagy Abrogates Nanoparticle-Induced Liver Injury." in: Advanced healthcare materials, Vol. 6, Issue 9, 2017 (PubMed).

Inoue, Tanaka, Masuda, Ohue-Kitano, Yamakage, Muranaka, Wada, Kusakabe, Shimatsu, Hasegawa, Satoh-Asahara: "Omega-3 polyunsaturated fatty acids suppress the inflammatory responses of lipopolysaccharide-stimulated mouse microglia by activating SIRT1 pathways." in: Biochimica et biophysica acta, Vol. 1862, Issue 5, pp. 552-560, 2017 (PubMed).

Lee, Probst, Santoyo-Sánchez, Al-Hamdani, Diebels, von Sivers, Kerek, Prenner, Thévenod: "Initial autophagic protection switches to disruption of autophagic flux by lysosomal instability during cadmium stress accrual in renal NRK-52E cells." in: Archives of toxicology, 2017 (PubMed).

Xu, Tian, Sun, Zhang, Ren, Fan, Wang, Wang, Yan, Chen, Shi, Dong: "FBXW7-Induced MTOR Degradation Forces Autophagy to Counteract Persistent Prion Infection." in: Molecular neurobiology, Vol. 53, Issue 1, pp. 706-19, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Lin: "In Vitro and in Vivo Atheroprotective Effects of Gossypetin against Endothelial Cell Injury by Induction of Autophagy." in: Chemical research in toxicology, Vol. 28, Issue 2, pp. 202-15, 2016 (PubMed).

Chesser, Ganeshan, Yang, Johnson: "Epigallocatechin-3-gallate enhances clearance of phosphorylated tau in primary neurons." in: Nutritional neuroscience, Vol. 19, Issue 1, pp. 21-31, 2016 (PubMed).