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Details for Product No. ABIN250638

Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody

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Synonyms ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a, Map1lc3, Mpl3, zbs559, 1010001C15Rik, Atg8, LC3b, zgc:56434, wu:fb60g11, map1lc3b, MGC76283, MAP1LC3B
»Alternatives Human
»Alternatives Rabbit
Clonality Polyclonal
»Alternatives Un-conjugated
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 13 references available
Catalog no. ABIN250638
Quantity 0.1 mL
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Immunogen A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence(between residues 1-100).
Specificity This antibody is specific for LC3. Species Reactivity: This antibody reacts with human and mouse LC3 protein, though the mouse detection isweaker than the human. No other species have been tested. The immunogen used forthis antibody production has 100% homology with rat and Zebrafish, 92% with mouse,91% with cow, and 84% with Xenopus proteins. Localization: LC3-I is cytoplasmic. LC3-II binds to the autophagic membranes.
Cross-Reactivity Human, Mouse (Murine)
Purification Aff - Purified
Alternative Name LC3B
Background LC3, a mammalian homologue of Apg8, was originally identified asmicrotubule-associated protein 1 light chain 3. It is a component of both the MAP1Aand MAP1B microtubule-binding domains and the heavy-chain independent regulationof LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic andLC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II isthe first mammalian protein identified that specifically associates with theautophagosome membranes. Alternate Names: anti-Microtubule-associated proteins 1A/1B light chain 3B MAP1A/MAP1B LC3 Bantibody, anti-MAP1A/b light chain 3 B antibody, anti-MAP1 light chain 3-like protein 2antibody, anti-Autophagy-related protein LC3 B antibody, anti-Autophagy-relatedubiquitin-like modifier LC3 B antibody.
Gene ID 84557, 66734, 64862
UniProt Q9GZQ8
Research Area Cancer, Autophagy, Cytoskeleton
Application Notes Suggested working dilutions: immunohistochemistry 1:200-1:400,immunoprecipitation 20ug/500ug of protein,Western Blot 2 ug/ml,Immunohistochemistry-Paraffin . Positive Controls: Human/mouse brain lysate, ES, HeLa, HEK293, MCF7 cells, human lymphoblasts, andembryoid bodies
Restrictions For Research Use only
Format Liquid
Concentration 1.16 mg/ml
Buffer PBS, 30% Glycerol. Preservative: 0.1% sodium azide.
Preservative Sodium azide
Storage -20 °C
Mizushima, Yamamoto, Hatano et al.: "Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells." in: The Journal of cell biology, Vol. 152, Issue 4, pp. 657-68, 2001 (PubMed).

Settembre, Fraldi, Jahreiss et al.: "A block of autophagy in lysosomal storage disorders." in: Human molecular genetics, Vol. 17, Issue 1, pp. 119-29, 2007 (PubMed).

Sarkar, Krishna, Imarisio et al.: "A rational mechanism for combination treatment of Huntington's disease using lithium and rapamycin." in: Human molecular genetics, Vol. 17, Issue 2, pp. 170-8, 2008 (PubMed).

Miller, Zhao, Stephenson et al.: "The autophagy gene ATG5 plays an essential role in B lymphocyte development." in: Autophagy, Vol. 4, Issue 3, pp. 309-14, 2008 (PubMed).

Wong, Tan, Soong et al.: "Autophagy-mediated clearance of aggresomes is not a universal phenomenon." in: Human molecular genetics, Vol. 17, Issue 16, pp. 2570-82, 2008 (PubMed).

Burdette, Yarbrough, Orvedahl et al.: "Vibrio parahaemolyticus orchestrates a multifaceted host cell infection by induction of autophagy, cell rounding, and then cell lysis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 34, pp. 12497-502, 2008 (PubMed).

Degtyarev, De Maziuere, Orr et al.: "Akt inhibition promotes autophagy and sensitizes PTEN-null tumors to lysosomotropic agents." in: The Journal of cell biology, Vol. 183, Issue 1, pp. 101-16, 2008 (PubMed).

Cadwell, Liu, Brown et al.: "A key role for autophagy and the autophagy gene Atg16l1 in mouse and human intestinal Paneth cells." in: Nature, Vol. 456, Issue 7219, pp. 259-63, 2008 (PubMed).

Young, Martinez, La Spada: "Nutrient deprivation induces neuronal autophagy and implicates reduced insulin signaling in neuroprotective autophagy activation." in: The Journal of biological chemistry, Vol. 284, Issue 4, pp. 2363-73, 2009 (PubMed).

Humbey, Pimkina, Zilfou et al.: "The ARF tumor suppressor can promote the progression of some tumors." in: Cancer research, Vol. 68, Issue 23, pp. 9608-13, 2008 (PubMed).

Yuan, Perry, Huang et al.: "LPS-induced autophagy is mediated by oxidative signaling in cardiomyocytes and is associated with cytoprotection." in: American journal of physiology. Heart and circulatory physiology, Vol. 296, Issue 2, pp. H470-9, 2009 (PubMed).

Panyasrivanit, Khakpoor, Wikan et al.: "Co-localization of constituents of the dengue virus translation and replication machinery with amphisomes." in: The Journal of general virology, Vol. 90, Issue Pt 2, pp. 448-56, 2009 (PubMed).

Sarkar, Korolchuk, Renna et al.: "Methodological considerations for assessing autophagy modulators: a study with calcium phosphate precipitates." in: Autophagy, Vol. 5, Issue 3, pp. 307-13, 2009 (PubMed).

Alternatives for antigen "Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B)", type "Antibodies"
Hosts (83), (13), (2)
Reactivities (95), (57), (42), (27), (26), (14), (13), (3), (2), (2), (1)
Applications (64), (29), (26), (26), (20), (19), (17), (12), (8), (8), (7), (1), (1)
Conjugates (6), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Epitopes (17), (6), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)