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Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody

Details for Product No. ABIN250643, Supplier: Log in to see
Antigen
  • Map1lc3
  • zgc:56434
  • wu:fb60g11
  • map1lc3b
  • MGC76283
  • MAP1LC3B
  • ATG8F
  • LC3B
  • MAP1A/1BLC3
  • MAP1LC3B-a
  • 1010001C15Rik
  • Atg8
  • LC3b
  • Mpl3
  • zbs559
  • microtubule-associated protein 1 light chain 3 beta
  • map1lc3b
  • MAP1LC3B
  • LOC100361973
  • Map1lc3b
Epitope
N-Term, AA 1-100
68
33
23
22
22
17
11
10
9
8
8
6
6
5
5
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Bacteria, Cow (Bovine), Dog (Canine), Golden Syrian Hamster, Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus), Zebrafish
256
142
92
32
27
27
26
25
12
12
6
6
1
Host
Rabbit
239
20
1
Clonality
Polyclonal
Conjugate
Un-conjugated
17
15
14
13
13
13
4
4
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Simple Western (SimWes), Western Blotting (WB)
144
125
80
75
70
62
56
37
26
10
9
6
3
2
2
1
1
1
Options
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Immunogen A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1-100). [UniProt# Q9GZQ8]
Purification Immunogen affinity purified
Alternative Name LC3B/MAP1LC3B (MAP1LC3B Antibody Abstract)
Background Gene Symbol: MAP1LC3B
Gene ID 81631
UniProt Q9GZQ8
Research Area Cytoskeleton, Autophagy, Cancer
Application Notes Western Blot 0.5-2 μg/mL, Simple Western 1:50, ELISA, Immunohistochemistry 1:200-1:400, Immunocytochemistry/Immunofluorescence 1:200, Immunoprecipitation 20 μg/500 μg of protein, Immunohistochemistry-Paraffin 1:200-1:400, Immunohistochemistry-Frozen, Proximity Ligation AssayThis LC3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, Immunoprecipitation and Western Blot. Use in Immunohistochemistry-Frozen sections reported in scientific literature (PMID: 20008275). Use in ELISA reported in scientific literature (PMID 20503249). In Western blot bands are seen ~17 and 19 kDa corresponding to LC3-II and LC3-I. In some cases a non-specific band is seen at ~21 kDa in mouse protein. In ICC/IF, cytoplasmic staining was observed in HeLa cells. Use in Proximity Ligation Assay reported in scientific literature (PMID 27219062). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot Protocol protocol specific for LC3 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 1 hour at room temperature (RT).
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-LC3 primary antibody in blocking buffer and incubate 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (we used BioFX Super Plus ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.I. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. II. Quench Endogenous Peroxidase:To Prepare 200 mL of Quenching Solution: Hydrogen Peroxide to 200 mL of Methanol.**Use within 4 hours of preparationA. Place slides in peroxidase quenching solution: 15-30 minutes.B. Place slides in distilled water: 2 changes for 2 minutes each. III. Retrieve Epitopes: A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250643) Western Blot: LC3 Antibody [ABIN250638SS] - Detection of LC3 in human brain lysate us...
Immunohistochemistry (IHC) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250643) Imnmunohistochemistry: LC3 Antibody [ABIN250638SS] - Staining of brain, cerebral cort...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250643) Detection of LC3 in human brain lysate using ABIN250638. Atg5-/- ES cells from Dr. No...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250643) Immunocytochemistry/Immunofluorescence: LC3B/MAP1LC3B Antibody [ABIN250638] - Analysi...
Simple Western (SimWes) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250643) Simple Western: LC3B/MAP1LC3B Antibody [ABIN250638] - Simple Western lane view shows ...
Immunohistochemistry (IHC) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250643) Immunohistochemistry: LC3B/MAP1LC3B Antibody [ABIN250638] - IHC of rat brain from con...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250643) Western Blot: LC3B/MAP1LC3B Antibody [ABIN250638] - Analysis of LC3 in H9C2 cells.
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250643) Western Blot: LC3B/MAP1LC3B Antibody [ABIN250638] - Analysis using the Biotin conjuga...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250643) Western Blot: LC3B/MAP1LC3B Antibody [ABIN250638] - Detection of LC3 in mouse HL-1 ce...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250643) Western Blot: LC3B/MAP1LC3B Antibody [ABIN250638] - Western blot analysis of HeLa (1)...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250643) Immunohistochemistry-Paraffin: LC3B/MAP1LC3B Antibody [ABIN250638] - Human ovarian Ca...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250643) Western Blot: LC3B/MAP1LC3B Antibody [ABIN250638] - analysis of LC3 in Huh-7 and SMMC...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250643) Western Blot: LC3B/MAP1LC3B Antibody [ABIN250638] - Analysis using the DyLight 550 co...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN250643) Immunocytochemistry/Immunofluorescence: LC3B/MAP1LC3B Antibody [ABIN250638] - LC3 ant...
Product cited in: Xu, Tian, Sun, Zhang, Ren, Fan, Wang, Wang, Yan, Chen, Shi, Dong: "FBXW7-Induced MTOR Degradation Forces Autophagy to Counteract Persistent Prion Infection." in: Molecular neurobiology, Vol. 53, Issue 1, pp. 706-19, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Lin: "In Vitro and in Vivo Atheroprotective Effects of Gossypetin against Endothelial Cell Injury by Induction of Autophagy." in: Chemical research in toxicology, Vol. 28, Issue 2, pp. 202-15, 2016 (PubMed).

Chesser, Ganeshan, Yang, Johnson: "Epigallocatechin-3-gallate enhances clearance of phosphorylated tau in primary neurons." in: Nutritional neuroscience, Vol. 19, Issue 1, pp. 21-31, 2016 (PubMed).

Sorrell, Golder, Johnstone, Karet Frankl: "Renal peroxiredoxin 6 interacts with anion exchanger 1 and plays a novel role in pH homeostasis." in: Kidney international, Vol. 89, Issue 1, pp. 105-12, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Jiang, Li, Tang, Wang, Liu, Guo: "Bakkenolide-IIIa Protects Against Cerebral Damage Via Inhibiting NF-κB Activation." in: CNS neuroscience & therapeutics, Vol. 21, Issue 12, pp. 943-52, 2016 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Zhang, Zhou, Lin, Wei, Zhang, Jin, Chen, Man, Wen: "Autophagic lysosomal reformation depends on mTOR reactivation in H2O2-induced autophagy." in: The international journal of biochemistry & cell biology, Vol. 70, pp. 76-81, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Fritzen, Madsen, Kleinert, Treebak, Lundsgaard, Jensen, Richter, Wojtaszewski, Kiens, Frøsig: "Regulation of autophagy in human skeletal muscle: effects of exercise, exercise training and insulin stimulation." in: The Journal of physiology, Vol. 594, Issue 3, pp. 745-61, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Stotland, Gottlieb: "α-MHC MitoTimer mouse: In vivo mitochondrial turnover model reveals remarkable mitochondrial heterogeneity in the heart." in: Journal of molecular and cellular cardiology, Vol. 90, pp. 53-8, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Avagliano, Doi, Tosi, Scagliotti, Gualtieri, Gaston-Massuet, Pistocchi, Gallina, Marconi, Bulfamante, Massa: "Cell death and cell proliferation in human spina bifida." in: Birth defects research. Part A, Clinical and molecular teratology, Vol. 106, Issue 2, pp. 104-13, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Lee, Lee, Yim, Lee, Pyo: "Ramalin-Mediated Apoptosis Is Enhanced by Autophagy Inhibition in Human Breast Cancer Cells." in: Phytotherapy research : PTR, Vol. 30, Issue 3, pp. 426-38, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Kraft, Manral, Dowler, Kenworthy: "Nuclear LC3 Associates with Slowly Diffusing Complexes that Survey the Nucleolus." in: Traffic (Copenhagen, Denmark), Vol. 17, Issue 4, pp. 369-99, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Mukherjee, Chakrabarti: "Ubiquitin-mediated regulation of the E3 ligase GP78 by MGRN1 in trans affects mitochondrial homeostasis." in: Journal of cell science, Vol. 129, Issue 4, pp. 757-73, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Yang, Licastro, Cava, Veronese, Spelta, Rizza, Bertozzi, Villareal, Hotamisligil, Holloszy, Fontana: "Long-Term Calorie Restriction Enhances Cellular Quality-Control Processes in Human Skeletal Muscle." in: Cell reports, Vol. 14, Issue 3, pp. 422-8, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Tian, Sun, Wu, Pei, Zhang, Zhang, Wang, Li, Wang, Shi, Hu, Fan: "Acupuncture promotes mTOR-independent autophagic clearance of aggregation-prone proteins in mouse brain." in: Scientific reports, Vol. 6, pp. 19714, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Wu, Fleming, Ricketts, Pavel, Virgin, Menzies, Rubinsztein: "Autophagy regulates Notch degradation and modulates stem cell development and neurogenesis." in: Nature communications, Vol. 7, pp. 10533, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Liu, Wang, Wang, Nie, Du, Dai, Wang, Wang, Chen, Hei, Deng, Wu, Xu: "Graphene Oxide Attenuates the Cytotoxicity and Mutagenicity of PCB 52 via Activation of Genuine Autophagy." in: Environmental science & technology, Vol. 50, Issue 6, pp. 3154-64, 2016 (PubMed). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Fritzen, Frøsig, Jeppesen, Jensen, Lundsgaard, Serup, Schjerling, Proud, Richter, Kiens: "Role of AMPK in regulation of LC3 lipidation as a marker of autophagy in skeletal muscle." in: Cellular signalling, Vol. 28, Issue 6, pp. 663-74, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Wang, Du, Dong, Li, Wu, Zhang: "Autophagy inhibition facilitates erlotinib cytotoxicity in lung cancer cells through modulation of endoplasmic reticulum stress." in: International journal of oncology, Vol. 48, Issue 6, pp. 2558-66, 2016 (PubMed).

Zhou, Wang, Zhou, Chen: "Autophagy activation prevents sevoflurane-induced neurotoxicity in H4 human neuroglioma cells." in: Acta pharmacologica Sinica, Vol. 37, Issue 5, pp. 580-8, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

He, Weber, Schilling: "Glutamine Modulates Macrophage Lipotoxicity." in: Nutrients, Vol. 8, Issue 4, pp. 215, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting