Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody

Antigen: Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B)

Synonyms: LC3B, MAP1A/1BLC3, Mpl3, zbs559, Map1lc3, MGC93422, LC3b, MGC118046, 1010001C15Rik, zgc:56434, wu:fb60g11, map1lc3b, MGC76283, MAP1LC3B

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN250643

Additional Information

Alternative name: LC3B

Gene ID: 81631

Immunogen: A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence(between residues 1-100).

Cross-Reactivity: Human, Mouse (Murine)

Description: LC3, a mammalian homologue of Apg8, was originally identified asmicrotubule-associated protein 1 light chain 3. It is a component of both the MAP1Aand MAP1B microtubule-binding domains and the heavy-chain independent regulationof LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic andLC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II isthe first mammalian protein identified that specifically associates with theautophagosome membranes. Alternate Names: anti-Microtubule-associated proteins 1A/1B light chain 3B MAP1A/MAP1B LC3 Bantibody, anti-MAP1A/b light chain 3 B antibody, anti-MAP1 light chain 3-like protein 2antibody, anti-Autophagy-related protein LC3 B antibody, anti-Autophagy-relatedubiquitin-like modifier LC3 B antibody.

Specificity: This antibody is specific for LC3. Species Reactivity: This antibody reacts with human and mouse LC3 protein, though the mouse detection isweaker than the human. No other species have been tested. The immunogen used forthis antibody production has 100% homology with rat and Zebrafish, 92% with mouse,91% with cow, and 84% with Xenopus proteins. Localization: LC3-I is cytoplasmic. LC3-II binds to the autophagic membranes.

Application Details

Application Notes: Suggested working dilutions: immunoprecipitation 20ug/500ug of protein,Western Blot 2 ug/ml,Immunohistochemistry-Paraffin . Positive Controls: Human and mouse brain lysate, ES, HeLa, HEK293, and MCF7 cells, humanlymphoblasts, embryoid bodies

Concentration: 1.48 mg/ml

Purification: Aff - Purified

Buffer: PBS, 30% Glycerol. Preservative: 0.1% sodium azide

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Research Area: Cancer, Autophagy, Cytoskeleton

Restrictions: For Research Use only

Images

Western Blot: LC3 Antibody [ABIN250638SS] - Detection of LC3 in human brain lysate using ABIN250638. Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center

Imnmunohistochemistry: LC3 Antibody [ABIN250638SS] - Staining of brain, cerebral cortex, neurons with cell processes using ABIN250638.

Detection of LC3 in human brain lysate using ABIN250638. Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center

Publications

Settembre, Fraldi, Jahreiss et al.: "A block of autophagy in lysosomal storage disorders." in: Human molecular genetics, Vol. 17, Issue 1, pp. 119-29, 2007 (PubMed).

Sarkar, Krishna, Imarisio et al.: "A rational mechanism for combination treatment of Huntington's disease using lithium and rapamycin." in: Human molecular genetics, Vol. 17, Issue 2, pp. 170-8, 2008 (PubMed).

Miller, Zhao, Stephenson et al.: "The autophagy gene ATG5 plays an essential role in B lymphocyte development." in: Autophagy, Vol. 4, Issue 3, pp. 309-14, 2008 (PubMed).

Wong, Tan, Soong et al.: "Autophagy-mediated clearance of aggresomes is not a universal phenomenon." in: Human molecular genetics, Vol. 17, Issue 16, pp. 2570-82, 2008 (PubMed).

Burdette, Yarbrough, Orvedahl et al.: "Vibrio parahaemolyticus orchestrates a multifaceted host cell infection by induction of autophagy, cell rounding, and then cell lysis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 34, pp. 12497-502, 2008 (PubMed).

Degtyarev, De Maziuere, Orr et al.: "Akt inhibition promotes autophagy and sensitizes PTEN-null tumors to lysosomotropic agents." in: The Journal of cell biology, Vol. 183, Issue 1, pp. 101-16, 2008 (PubMed).

Cadwell, Liu, Brown et al.: "A key role for autophagy and the autophagy gene Atg16l1 in mouse and human intestinal Paneth cells." in: Nature, Vol. 456, Issue 7219, pp. 259-63, 2008 (PubMed).

Young, Martinez, La Spada: "Nutrient deprivation induces neuronal autophagy and implicates reduced insulin signaling in neuroprotective autophagy activation." in: The Journal of biological chemistry, Vol. 284, Issue 4, pp. 2363-73, 2009 (PubMed).

Humbey, Pimkina, Zilfou et al.: "The ARF tumor suppressor can promote the progression of some tumors." in: Cancer research, Vol. 68, Issue 23, pp. 9608-13, 2008 (PubMed).

Yuan, Perry, Huang et al.: "LPS-induced autophagy is mediated by oxidative signaling in cardiomyocytes and is associated with cytoprotection." in: American journal of physiology. Heart and circulatory physiology, Vol. 296, Issue 2, pp. H470-9, 2009 (PubMed).