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ERN1 antibody (Endoplasmic Reticulum (ER) To Nucleus Signalling 1) (AA 700-800)

Details for Product anti-ERN1 Antibody No. ABIN250711, Supplier: Log in to see
Antigen
Epitope
AA 700-800
13
13
13
Reactivity
Human, Mouse (Murine), Rat (Rattus)
40
39
26
8
8
8
8
8
Host
Rabbit
39
1
Clonality
Polyclonal
Conjugate
This ERN1 antibody is un-conjugated
6
6
3
3
3
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
40
7
7
6
6
4
4
Supplier
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Immunogen A synthetic peptide within the human IRE1 alpha protein (within residues 700-800). [Swiss-Prot #O75460]
Specificity This antibody detects the levels of endogenous total IRE1 Alpha protein.
Purification Immunogen affinity purified
Alternative Name IRE1 alpha
Background Gene Symbol: ERN1
Molecular Weight Theoretical MW: 110 kDa
UniProt O75460
Application Notes Western Blot 1:1000-1:2000, Immunohistochemistry 1:250-1:500, Immunocytochemistry/Immunofluorescence 1:100 - 1:250, Immunohistochemistry-Paraffin 1:250-1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol ICC/IF Protocol for IRE1 alpha Antibody Immunocytochemistry ProtocolCulture cells to appropriate density on suitable glass coverslips in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 5-10 minutes.
. Remove the formalin and add 0.5 % Triton-X 100 in TBS to permeabilize the cells. Incubate for 5-10 minutes.
. Remove the permeabilization buffer and add wash buffer (i.e. PBS or PBS with 0.1 % Tween-20). Be sure to not let the specimen dry out. Gently wash three times for 10 minutes.
. Alternatively, cells can be fixed with -20C methanol for 10 min at room temperature. Remove the methanol and rehydrate in PBS for 10 min before proceeding.
. To block nonspecific antibody binding incubate in 10 % normal goat serum for 1 hour at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature for 1 hour or at 4 degrees C overnight.
. Remove primary antibody and replace with wash buffer. Gently wash three times for 10 minutes.
. Add secondary antibody at the appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash buffer. Gently wash three times for 10 minutes.
. Nuclei can be staining with 4',6' diamino phenylindole (DAPI) at 0.1 µg/ml, or coverslips can be directly mounted in media containing DAPI.
. Cells can now be viewed with a fluorescence microscope.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow proper laboratory procedures for the disposal of formalin.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-glycine, 150 mM NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-ERN1 antibody (Endoplasmic Reticulum (ER) To Nucleus Signalling 1) (AA 700-800) (ABIN250711) Detection of Ire1 alpha in transfected Min6 cells using ABIN250711.
Western Blotting (WB) image for anti-ERN1 antibody (Endoplasmic Reticulum (ER) To Nucleus Signalling 1) (AA 700-800) (ABIN250711) Western Blot: IRE1 alpha Antibody [ABIN250711] - Western Blot detection of Total IRE1...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ERN1 antibody (Endoplasmic Reticulum (ER) To Nucleus Signalling 1) (AA 700-800) (ABIN250711) Immunohistochemistry-Paraffin: IRE1 alpha Antibody [ABIN250711] - IHC-P analysis of a...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ERN1 antibody (Endoplasmic Reticulum (ER) To Nucleus Signalling 1) (AA 700-800) (ABIN250711) Immunohistochemistry-Paraffin: IRE1 alpha Antibody [ABIN250711] - IHC-P analysis of a...
Immunofluorescence (IF) image for anti-ERN1 antibody (Endoplasmic Reticulum (ER) To Nucleus Signalling 1) (AA 700-800) (ABIN250711) Immunocytochemistry/Immunofluorescence: IRE1 alpha Antibody [ABIN250711] - The IRE-1 ...
Product cited in: Alnasser, Guan, Zhang, Gleave, Nguan, Du: "Requirement of clusterin expression for prosurvival autophagy in hypoxic kidney tubular epithelial cells." in: American journal of physiology. Renal physiology, Vol. 310, Issue 2, pp. F160-73, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Kim, Jun, Lee: "Effect of Cudrania tricuspidata and Kaempferol in Endoplasmic Reticulum Stress-Induced Inflammation and Hepatic Insulin Resistance in HepG2 Cells." in: Nutrients, Vol. 8, Issue 1, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Vannuvel, Van Steenbrugge, Demazy, Ninane, Fattaccioli, Fransolet, Renard, Raes, Arnould: "Effects of a Sublethal and Transient Stress of the Endoplasmic Reticulum on the Mitochondrial Population." in: Journal of cellular physiology, Vol. 231, Issue 9, pp. 1913-31, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Li, Zalzala, Jadhav, Xu, Kasumov, Yin, Zhang: "Carboxylesterase 2 prevents liver steatosis by modulating lipolysis, endoplasmic reticulum stress, and lipogenesis and is regulated by hepatocyte nuclear factor 4 alpha in mice." in: Hepatology (Baltimore, Md.), Vol. 63, Issue 6, pp. 1860-74, 2016 (PubMed).

Ghosh, Garg, Mau, OBrien, Liu, Yung: "Elevated Endoplasmic Reticulum Stress Response Contributes to Adipose Tissue Inflammation in Aging." in: The journals of gerontology. Series A, Biological sciences and medical sciences, Vol. 70, Issue 11, pp. 1320-9, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Clarke, Lyra E Silva, Figueiredo, Frozza, Ledo, Beckman, Katashima, Razolli, Carvalho, Frazão, Silveira, Ribeiro, Bomfim, Neves, Klein, Medeiros, LaFerla, Carvalheira, Saad, Munoz, Velloso, Ferreira et al.: "Alzheimer-associated Aβ oligomers impact the central nervous system to induce peripheral metabolic deregulation. ..." in: EMBO molecular medicine, Vol. 7, Issue 2, pp. 190-210, 2015 (PubMed). Further details: Western Blotting

Quan, Wang, Liang, Zheng, Zhang: "Melatonin inhibits tunicamycin-induced endoplasmic reticulum stress and insulin resistance in skeletal muscle cells." in: Biochemical and biophysical research communications, Vol. 463, Issue 4, pp. 1102-7, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Liu, Fan, Tang, Ke: "The resveratrol attenuates ethanol-induced hepatocyte apoptosis via inhibiting ER-related caspase-12 activation and PDE activity in vitro." in: Alcoholism, clinical and experimental research, Vol. 38, Issue 3, pp. 683-93, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Wu, Yang, Fan, Peng, Deng, Ding, Yang, Zhou, Miao, Fu: "Deficiency of osteoblastic Arl6ip5 impaired osteoblast differentiation and enhanced osteoclastogenesis via disturbance of ER calcium homeostasis and induction of ER stress-mediated apoptosis." in: Cell death & disease, Vol. 5, pp. e1464, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Zheng, Zhang, Joe, Kim, Uddin, Rhew, Kim, Ryter, Chung: "Carbon monoxide-releasing molecules reverse leptin resistance induced by endoplasmic reticulum stress." in: American journal of physiology. Endocrinology and metabolism, Vol. 304, Issue 7, pp. E780-8, 2013 (PubMed). Further details: Western Blotting

Arshad, Ye, Gu, Wong, Liu, Li, Zhou, Zhang, Bay, Yu, Li: "RNF13, a RING finger protein, mediates endoplasmic reticulum stress-induced apoptosis through the inositol-requiring enzyme (IRE1α)/c-Jun NH2-terminal kinase pathway." in: The Journal of biological chemistry, Vol. 288, Issue 12, pp. 8726-36, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Kurata, Yamazaki, Kanno, Ishibashi, Takahara, Kitagawa, Nakamura: "Anti-apoptotic function of Xbp1 as an IL-3 signaling molecule in hematopoietic cells." in: Cell death & disease, Vol. 2, pp. e118, 2011 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Luo, He, Zhang, Yu, Chen, Xu, Tang, Urano, Min: "AIP1 is critical in transducing IRE1-mediated endoplasmic reticulum stress response." in: The Journal of biological chemistry, Vol. 283, Issue 18, pp. 11905-12, 2008 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Lipson, Fonseca, Ishigaki, Nguyen, Foss, Bortell, Rossini, Urano: "Regulation of insulin biosynthesis in pancreatic beta cells by an endoplasmic reticulum-resident protein kinase IRE1." in: Cell metabolism, Vol. 4, Issue 3, pp. 245-54, 2006 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Fonseca, Fukuma, Lipson, Nguyen, Allen, Oka, Urano: "WFS1 is a novel component of the unfolded protein response and maintains homeostasis of the endoplasmic reticulum in pancreatic beta-cells." in: The Journal of biological chemistry, Vol. 280, Issue 47, pp. 39609-15, 2005 (PubMed).

Kubota, Lee, Tsuchiya, Young, Everett, Martinez-Mier, Snead, Nguyen, Urano, Bartlett: "Fluoride induces endoplasmic reticulum stress in ameloblasts responsible for dental enamel formation." in: The Journal of biological chemistry, Vol. 280, Issue 24, pp. 23194-202, 2005 (PubMed).