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ERN1 antibody (Endoplasmic Reticulum (ER) To Nucleus Signalling 1) (AA 700-800)

Details for Product anti-ERN1 Antibody No. ABIN250711, Supplier: Log in to see
Antigen
Epitope
AA 700-800
17
17
16
Reactivity
Human, Mouse (Murine), Rat (Rattus)
53
50
33
12
12
12
12
12
Host
Rabbit
51
1
1
Clonality
Polyclonal
Conjugate
This ERN1 antibody is un-conjugated
6
6
6
6
6
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
50
24
21
21
14
13
12
1
Options
Supplier
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Immunogen A synthetic peptide within the human IRE1 alpha protein (within residues 700-800). [Swiss-Prot #O75460]
Specificity This antibody detects the levels of endogenous total IRE1 Alpha protein.
Purification Immunogen affinity purified
Alternative Name IRE1 alpha
Background Gene Symbol: ERN1
Molecular Weight Theoretical MW: 110 kDa
Gene ID 2081
UniProt O75460
Application Notes Western Blot 1:1000-1:2000, Immunohistochemistry 1:250-1:500, Immunocytochemistry/Immunofluorescence 1:100 - 1:250, Immunohistochemistry-Paraffin 1:250-1:500This IRE1 alpha antibody is useful for Western blot, where a band ~110 kDa is observed. In ICC/IF endoplasmic reticulum staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol ICC/IF Protocol for IRE1 alpha Antibody Immunocytochemistry ProtocolCulture cells to appropriate density on suitable glass coverslips in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 5-10 minutes.
. Remove the formalin and add 0.5 % Triton-X 100 in TBS to permeabilize the cells. Incubate for 5-10 minutes.
. Remove the permeabilization buffer and add wash buffer (i.e. PBS or PBS with 0.1 % Tween-20). Be sure to not let the specimen dry out. Gently wash three times for 10 minutes.
. Alternatively, cells can be fixed with -20C methanol for 10 min at room temperature. Remove the methanol and rehydrate in PBS for 10 min before proceeding.
. To block nonspecific antibody binding incubate in 10 % normal goat serum for 1 hour at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature for 1 hour or at 4 degrees C overnight.
. Remove primary antibody and replace with wash buffer. Gently wash three times for 10 minutes.
. Add secondary antibody at the appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash buffer. Gently wash three times for 10 minutes.
. Nuclei can be staining with 4',6' diamino phenylindole (DAPI) at 0.1 µg/ml, or coverslips can be directly mounted in media containing DAPI.
. Cells can now be viewed with a fluorescence microscope.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow proper laboratory procedures for the disposal of formalin.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-ERN1 antibody (Endoplasmic Reticulum (ER) To Nucleus Signalling 1) (AA 700-800) (ABIN250711) Detection of Ire1 alpha in transfected Min6 cells using ABIN250711.
Western Blotting (WB) image for anti-ERN1 antibody (Endoplasmic Reticulum (ER) To Nucleus Signalling 1) (AA 700-800) (ABIN250711) Western Blot: IRE1 alpha Antibody [ABIN250711] - Western Blot detection of Total IRE1...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ERN1 antibody (Endoplasmic Reticulum (ER) To Nucleus Signalling 1) (AA 700-800) (ABIN250711) Immunohistochemistry-Paraffin: IRE1 alpha Antibody [ABIN250711] - IHC-P analysis of a...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ERN1 antibody (Endoplasmic Reticulum (ER) To Nucleus Signalling 1) (AA 700-800) (ABIN250711) Immunohistochemistry-Paraffin: IRE1 alpha Antibody [ABIN250711] - IHC-P analysis of a...
Immunofluorescence (IF) image for anti-ERN1 antibody (Endoplasmic Reticulum (ER) To Nucleus Signalling 1) (AA 700-800) (ABIN250711) Immunocytochemistry/Immunofluorescence: IRE1 alpha Antibody [ABIN250711] - The IRE-1 ...
Product cited in: Alnasser, Guan, Zhang, Gleave, Nguan, Du: "Requirement of clusterin expression for prosurvival autophagy in hypoxic kidney tubular epithelial cells." in: American journal of physiology. Renal physiology, Vol. 310, Issue 2, pp. F160-73, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Kim, Jun, Lee: "Effect of Cudrania tricuspidata and Kaempferol in Endoplasmic Reticulum Stress-Induced Inflammation and Hepatic Insulin Resistance in HepG2 Cells." in: Nutrients, Vol. 8, Issue 1, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Vannuvel, Van Steenbrugge, Demazy, Ninane, Fattaccioli, Fransolet, Renard, Raes, Arnould: "Effects of a Sublethal and Transient Stress of the Endoplasmic Reticulum on the Mitochondrial Population." in: Journal of cellular physiology, Vol. 231, Issue 9, pp. 1913-31, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Li, Zalzala, Jadhav, Xu, Kasumov, Yin, Zhang: "Carboxylesterase 2 prevents liver steatosis by modulating lipolysis, endoplasmic reticulum stress, and lipogenesis and is regulated by hepatocyte nuclear factor 4 alpha in mice." in: Hepatology (Baltimore, Md.), Vol. 63, Issue 6, pp. 1860-74, 2016 (PubMed).

Ghosh, Garg, Mau, OBrien, Liu, Yung: "Elevated Endoplasmic Reticulum Stress Response Contributes to Adipose Tissue Inflammation in Aging." in: The journals of gerontology. Series A, Biological sciences and medical sciences, Vol. 70, Issue 11, pp. 1320-9, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Clarke, Lyra E Silva, Figueiredo, Frozza, Ledo, Beckman, Katashima, Razolli, Carvalho, Frazão, Silveira, Ribeiro, Bomfim, Neves, Klein, Medeiros, LaFerla, Carvalheira, Saad, Munoz, Velloso, Ferreira et al.: "Alzheimer-associated Aβ oligomers impact the central nervous system to induce peripheral metabolic deregulation. ..." in: EMBO molecular medicine, Vol. 7, Issue 2, pp. 190-210, 2015 (PubMed). Further details: Western Blotting

Quan, Wang, Liang, Zheng, Zhang: "Melatonin inhibits tunicamycin-induced endoplasmic reticulum stress and insulin resistance in skeletal muscle cells." in: Biochemical and biophysical research communications, Vol. 463, Issue 4, pp. 1102-7, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Liu, Fan, Tang, Ke: "The resveratrol attenuates ethanol-induced hepatocyte apoptosis via inhibiting ER-related caspase-12 activation and PDE activity in vitro." in: Alcoholism, clinical and experimental research, Vol. 38, Issue 3, pp. 683-93, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Wu, Yang, Fan, Peng, Deng, Ding, Yang, Zhou, Miao, Fu: "Deficiency of osteoblastic Arl6ip5 impaired osteoblast differentiation and enhanced osteoclastogenesis via disturbance of ER calcium homeostasis and induction of ER stress-mediated apoptosis." in: Cell death & disease, Vol. 5, pp. e1464, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Zheng, Zhang, Joe, Kim, Uddin, Rhew, Kim, Ryter, Chung: "Carbon monoxide-releasing molecules reverse leptin resistance induced by endoplasmic reticulum stress." in: American journal of physiology. Endocrinology and metabolism, Vol. 304, Issue 7, pp. E780-8, 2013 (PubMed). Further details: Western Blotting

Arshad, Ye, Gu, Wong, Liu, Li, Zhou, Zhang, Bay, Yu, Li: "RNF13, a RING finger protein, mediates endoplasmic reticulum stress-induced apoptosis through the inositol-requiring enzyme (IRE1α)/c-Jun NH2-terminal kinase pathway." in: The Journal of biological chemistry, Vol. 288, Issue 12, pp. 8726-36, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Kurata, Yamazaki, Kanno, Ishibashi, Takahara, Kitagawa, Nakamura: "Anti-apoptotic function of Xbp1 as an IL-3 signaling molecule in hematopoietic cells." in: Cell death & disease, Vol. 2, pp. e118, 2011 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Luo, He, Zhang, Yu, Chen, Xu, Tang, Urano, Min: "AIP1 is critical in transducing IRE1-mediated endoplasmic reticulum stress response." in: The Journal of biological chemistry, Vol. 283, Issue 18, pp. 11905-12, 2008 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Lipson, Fonseca, Ishigaki, Nguyen, Foss, Bortell, Rossini, Urano: "Regulation of insulin biosynthesis in pancreatic beta cells by an endoplasmic reticulum-resident protein kinase IRE1." in: Cell metabolism, Vol. 4, Issue 3, pp. 245-54, 2006 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Fonseca, Fukuma, Lipson, Nguyen, Allen, Oka, Urano: "WFS1 is a novel component of the unfolded protein response and maintains homeostasis of the endoplasmic reticulum in pancreatic beta-cells." in: The Journal of biological chemistry, Vol. 280, Issue 47, pp. 39609-15, 2005 (PubMed).

Kubota, Lee, Tsuchiya, Young, Everett, Martinez-Mier, Snead, Nguyen, Urano, Bartlett: "Fluoride induces endoplasmic reticulum stress in ameloblasts responsible for dental enamel formation." in: The Journal of biological chemistry, Vol. 280, Issue 24, pp. 23194-202, 2005 (PubMed).