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Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (AA 700-800) antibody

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AA 700-800
(49), (16), (14), (13), (13), (13), (13), (13), (9), (8), (7), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(142), (53), (37), (3), (2), (2), (1), (1), (1), (1), (1)
(111), (31), (15), (2)
(9), (5), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
(130), (85), (22), (21), (14), (14), (11), (5), (4), (4), (4), (1), (1)
Pubmed 4 references available
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Quantity 0.1 mL
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Immunogen A synthetic peptide within the human IRE1 alpha protein (within residues 700-800).
No Cross-Reactivity Primate
Purification affinity purified
Alternative Name ERN1 / IRE1 (ERN1 Antibody Abstract)
Background IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of theunfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting itinto a potent unfolded-protein response transcriptional activator. It is a transmembraneprotein that has both serine-threonine kinase and endoribonuclease activities. Nullmutants are viable, but IRE1 is essential for viability under stress conditions that causeunfolded proteins to accumulate in the ER. Alternate Names: anti-Serine/threonine-protein kinase/endoribonuclease IRE1 antibody,anti-Inositol-requiring protein 1 antibody, anti-IRE1p antibody, anti-IRE1a antibody,anti-Ire1-alpha antibody, anti-ERN1 antibody, anti-Endoplasmic reticulum-to-nucleussignaling 1 antibody. Related Diseases: Neurodegenerative Disease, Cancer
Gene Symbol: ERN1
Gene ID 2081, 78943, 498013
UniProt O75460
Application Notes This IRE1 alpha antibody is useful for Western blot, where a band ~110 kDa is observed. In ICC/IF endoplasmic reticulum staining was observed in HeLa cells.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:100 - 1:250, Western Blot 1:1000-1:2000
Protocol ICC/IF Protocol for IRE1 alpha Antibody Immunocytochemistry ProtocolCulture cells to appropriate density on suitable glass coverslips in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 5-10 minutes.
. Remove the formalin and add 0.5 % Triton-X 100 in TBS to permeabilize the cells. Incubate for 5-10 minutes.
. Remove the permeabilization buffer and add wash buffer (i.e. PBS or PBS with 0.1 % Tween-20). Be sure to not let the specimen dry out. Gently wash three times for 10 minutes.
. Alternatively, cells can be fixed with -20C methanol for 10 min at room temperature. Remove the methanol and rehydrate in PBS for 10 min before proceeding.
. To block nonspecific antibody binding incubate in 10 % normal goat serum for 1 hour at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature for 1 hour or at 4 degrees C overnight.
. Remove primary antibody and replace with wash buffer. Gently wash three times for 10 minutes.
. Add secondary antibody at the appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash buffer. Gently wash three times for 10 minutes.
. Nuclei can be staining with 4',6' diamino phenylindole (DAPI) at 0.1 µg/ml, or coverslips can be directly mounted in media containing DAPI.
. Cells can now be viewed with a fluorescence microscope.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow proper laboratory procedures for the disposal of formalin.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-glycine, 150 mM NaCl, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (AA 700-800) antibody (ABIN250711) Detection of Ire1 alpha in transfected Min6 cells using ABIN250711.
Product cited in: Luo, He, Zhang et al.: "AIP1 is critical in transducing IRE1-mediated endoplasmic reticulum stress response." in: The Journal of biological chemistry, Vol. 283, Issue 18, pp. 11905-12, 2008 (PubMed).

Lipson, Fonseca, Ishigaki et al.: "Regulation of insulin biosynthesis in pancreatic beta cells by an endoplasmic reticulum-resident protein kinase IRE1." in: Cell metabolism, Vol. 4, Issue 3, pp. 245-54, 2006 (PubMed).

Fonseca, Fukuma, Lipson et al.: "WFS1 is a novel component of the unfolded protein response and maintains homeostasis of the endoplasmic reticulum in pancreatic beta-cells." in: The Journal of biological chemistry, Vol. 280, Issue 47, pp. 39609-15, 2005 (PubMed).

Kubota, Lee, Tsuchiya et al.: "Fluoride induces endoplasmic reticulum stress in ameloblasts responsible for dental enamel formation." in: The Journal of biological chemistry, Vol. 280, Issue 24, pp. 23194-202, 2005 (PubMed).

Catalog No. ABIN250711

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