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Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody

Details for Product No. ABIN250719, Supplier: Log in to see
Antigen
  • 1010001H21Rik
  • 4922501H04Rik
  • Anx3
  • ATG8E
  • b3gnt5
  • fb12f12
  • Lc3
  • LC3
  • LC3a
  • LC3A
  • LRRGT00047
  • MAP1ALC3
  • MAP1BLC3
  • Map1lc3
  • map1lc3a
  • MAP1LC3A
  • MGC69006
  • MGC89867
  • wu:fb12f12
  • wu:fb60g11
  • wu:fe50g08
  • zgc:56434
  • zgc:77094
Epitope
AA 25-121, Internal Region
43
38
28
26
24
23
14
14
14
14
13
13
12
12
11
10
8
8
7
7
6
6
5
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Fish, Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
317
109
103
32
30
16
14
10
9
6
6
5
3
1
1
Host
Rabbit
278
33
5
4
Clonality
Polyclonal
Conjugate
Un-conjugated
23
21
19
18
14
14
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Southern Blotting (SB), Western Blotting (WB)
258
145
137
79
65
32
27
23
19
12
10
10
6
1
Supplier
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Immunogen A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121). [UniProt# Q9GZQ8].
Specificity This antibody detects both LC3A and LC3B.
Cross-Reactivity (Details) Predicted to react with Xenopus,bovine based on 100% sequence homology.
Purification Immunogen affinity purified
Alternative Name LC3/MAP1LC3A (MAP1LC3A Antibody Abstract)
Background Gene Symbol: MAP1LC3A
UniProt Q9GZQ8
Application Notes Western Blot 2 μg/mL, Simple Western 1:50, Flow Cytometry, ELISA, Immunohistochemistry 1:200-1:400, Immunocytochemistry/Immunofluorescence 1:100-1:300, Immunoprecipitation 20 μg/ 500 μg of lysate, Immunohistochemistry-Paraffin 1:200-1:400, Immunohistochemistry-Frozen, Southern Blot
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot protocol specific for LC3 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 1 hour at room temperature (RT).
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-LC3 primary antibody in blocking buffer and incubate 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (we used BioFX Super Plus ECL). Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.I. Deparaffinization: A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase: To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.**Use within 4 hours of preparationA.Place slides in peroxidase quenching solution: 15-30 minutes.III. Retrieve Epitopes: A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody (ABIN250719) anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody
Immunohistochemistry (IHC) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody (ABIN250719) Staining of human brain, cerebral cortex, cell crocesses in gray matter using ABIN250...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody (ABIN250719) Immunohistochemistry-Paraffin: LC3/MAP1LC3A Antibody [ABIN250719] - Stainning in Mous...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody (ABIN250719) Western Blot: LC3/MAP1LC3A Antibody [ABIN250719] - Staining of HRP conjugated LC3 in ...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody (ABIN250719) Western Blot: LC3/MAP1LC3A Antibody [ABIN250719] - Analysis in human brain lyaste.
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody (ABIN250719) Western Blot: LC3/MAP1LC3A Antibody [ABIN250719] - Analysis in human lysates.
Simple Western (SimWes) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody (ABIN250719) Simple Western: LC3/MAP1LC3A Antibody [ABIN250719] - Simple Western lane view shows a...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody (ABIN250719) Immunohistochemistry-Paraffin: LC3/MAP1LC3A Antibody [ABIN250719] - Analysis in mouse...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody (ABIN250719) Immunocytochemistry/Immunofluorescence: LC3/MAP1LC3A Antibody [ABIN250719] - analysis...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody (ABIN250719) Immunocytochemistry/Immunofluorescence: LC3/MAP1LC3A Antibody [ABIN250719] - Analysis...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody (ABIN250719) Immunocytochemistry/Immunofluorescence: LC3/MAP1LC3A Antibody [ABIN250719] - Tested i...
Product cited in: Pomilio, Pavia, Gorojod, Vinuesa, Alaimo, Galvan, Kotler, Beauquis, Saravia: "Glial alterations from early to late stages in a model of Alzheimer's disease: Evidence of autophagy involvement in Aβ internalization." in: Hippocampus, Vol. 26, Issue 2, pp. 194-210, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

García-Prat, Martínez-Vicente, Perdiguero, Ortet, Rodríguez-Ubreva, Rebollo, Ruiz-Bonilla, Gutarra, Ballestar, Serrano, Sandri, Muñoz-Cánoves: "Autophagy maintains stemness by preventing senescence." in: Nature, Vol. 529, Issue 7584, pp. 37-42, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Li, Zou, Yue, Song, Chen, McKeehan, Wang, Xu, Huang, Yi, Liu: "Defects in MAP1S-mediated autophagy cause reduction in mouse lifespans especially when fibronectin is overexpressed." in: Aging cell, Vol. 15, Issue 2, pp. 370-9, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Hossini, Quast, Plötz, Grauel, Exner, Küchler, Stachelscheid, Eberle, Rabien, Makrantonaki, Zouboulis: "PI3K/AKT Signaling Pathway Is Essential for Survival of Induced Pluripotent Stem Cells." in: PLoS ONE, Vol. 11, Issue 5, pp. e0154770, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Erbil, Oral, Mitou, Kig, Durmaz-Timucin, Guven-Maiorov, Gulacti, Gokce, Dengjel, Sezerman, Gozuacik: "RACK1 Is an Interaction Partner of ATG5 and a Novel Regulator of Autophagy." in: The Journal of biological chemistry, Vol. 291, Issue 32, pp. 16753-65, 2016 (PubMed). Further details: Western Blotting

Lee, Kim, Quan, Lee, Kim, Kim, Bae, Hur, Lee: "Autophagy deficiency in myeloid cells increases susceptibility to obesity-induced diabetes and experimental colitis." in: Autophagy, Vol. 12, Issue 8, pp. 1390-403, 2016 (PubMed). Further details: Western Blotting

Suzuki, Yamaguchi, Yamanaka, Kanzaki, Kawashima, Togo, Katsuse, Koumitsu, Aoki, Iseki, Kosaka, Yamaguchi, Hashimoto, Aoki, Hirayasu: "Accumulated α-synuclein affects the progression of GM2 gangliosidoses." in: Experimental neurology, Vol. 284, Issue Pt A, pp. 38-49, 2016 (PubMed).

Saera-Vila, Kish, Louie, Grzegorski, Klionsky, Kahana: "Autophagy regulates cytoplasmic remodeling during cell reprogramming in a zebrafish model of muscle regeneration." in: Autophagy, Vol. 12, Issue 10, pp. 1864-1875, 2016 (PubMed). (Sample species: Zebrafish (Danio rerio)). Further details: Western Blotting

Wu, Wang, Zhang, Fang, Xu, Liu: "CpG-ODN promotes phagocytosis and autophagy through JNK/P38 signal pathway in Staphylococcus aureus-stimulated macrophage." in: Life sciences, Vol. 161, pp. 51-9, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Sabha, Volpatti, Gonorazky, Reifler, Davidson, Li, Eltayeb, DallArmi, Di Paolo, Brooks, Buj-Bello, Feldman, Dowling: "PIK3C2B inhibition improves function and prolongs survival in myotubular myopathy animal models." in: The Journal of clinical investigation, Vol. 126, Issue 9, pp. 3613-25, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Sun, Sun, Zhao, Li, Lin, Chen, Sun: "MAVS maintains mitochondrial homeostasis via autophagy." in: Cell discovery, Vol. 2, pp. 16024, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunoprecipitation

Tan, Ho, Cho, Yu, Hung, Yu: "Fucosylation of LAMP-1 and LAMP-2 by FUT1 correlates with lysosomal positioning and autophagic flux of breast cancer cells." in: Cell death & disease, Vol. 7, Issue 8, pp. e2347, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Saini, Baena, Ng, Venkataswamy, Kennedy, Kunnath-Velayudhan, Carreño, Xu, Chan, Larsen, Jacobs, Porcelli: "Suppression of autophagy and antigen presentation by Mycobacterium tuberculosis PE_PGRS47." in: Nature microbiology, Vol. 1, Issue 9, pp. 16133, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Mo, Li, Zhai, Gong: "Icariin Attenuates OGD/R-Induced Autophagy via Bcl-2-Dependent Cross Talk between Apoptosis and Autophagy in PC12 Cells." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 4343084, 2016 (PubMed). Further details: Western Blotting

Morell, Bort, Vara-Ciruelos, Ramos-Torres, Altamirano-Dimas, Díaz-Laviada, Rodríguez-Henche: "Up-Regulated Expression of LAMP2 and Autophagy Activity during Neuroendocrine Differentiation of Prostate Cancer LNCaP Cells." in: PLoS ONE, Vol. 11, Issue 9, pp. e0162977, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Granato, Zompetta, Vescarelli, Rizzello, Cardi, Valia, Antonelli, Marchese, Torrisi, Faggioni, Cirone: "HCV derived from sera of HCV-infected patients induces pro-fibrotic effects in human primary fibroblasts by activating GLI2." in: Scientific reports, Vol. 6, pp. 30649, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Hong, Kim, Lee: "Role of necroptosis in autophagy signaling during hepatic ischemia and reperfusion." in: Toxicology and applied pharmacology, Vol. 308, pp. 1-10, 2016 (PubMed). Further details: Western Blotting

Pema, Drusian, Chiaravalli, Castelli, Yao, Ricciardi, Somlo, Qian, Biffo, Boletta: "mTORC1-mediated inhibition of polycystin-1 expression drives renal cyst formation in tuberous sclerosis complex." in: Nature communications, Vol. 7, pp. 10786, 2016 (PubMed). Method employed by authors: Immunohistochemistry (IHC)

Zhang, Pan, Xiang, Liu, Dong, Livingston, Chen, Yin, Dong: "Protein Kinase Cδ Suppresses Autophagy to Induce Kidney Cell Apoptosis in Cisplatin Nephrotoxicity." in: Journal of the American Society of Nephrology : JASN, 2016 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Caccamo, Ferreira, Branca, Oddo: "p62 improves AD-like pathology by increasing autophagy." in: Molecular psychiatry, 2016 (PubMed). Method employed by authors: Western Blotting (WB)