Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 25-121) antibody (Biotin)

Details for Product No. ABIN250720
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Antigen
Synonyms Map1lc3, zgc:56434, wu:fb60g11, map1lc3b, MGC76283, MAP1LC3B, ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a, 1010001C15Rik, Atg8, LC3b, Mpl3, zbs559
Epitope
AA 25-121
(26), (11), (9), (9), (6), (6), (6), (5), (5), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(119), (45), (35), (24), (24), (14), (13), (4), (2), (1)
Host
Rabbit
(109), (14), (1)
Clonality
Polyclonal
Conjugate
Biotin
(9), (7), (7), (7), (6), (5), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(65), (50), (32), (27), (24), (20), (18), (14), (12), (6), (5), (2), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mL
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Catalog No. ABIN250720
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Immunogen A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121).
Specificity This antibody detects both LC3A and LC3B.
Cross-Reactivity (Details) Predicted to react with Xenopus,bovine based on 100% sequence homology.
Purification affinity purified
Alternative Name LC3B
Background Autophagy is a process of intracellular bulk degradation in which cytoplasmiccomponents, including organelles, are sequestered within double-membrane vesiclesthat deliver the contents to the lysosome/vacuole for degradation. Duringmacroautophagy, the sequestering vesicles, termed autophagosomes, fuse with thelysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) intothe lumen of the degradative compartment. There are 16 proteins participating in theautophagy pathway in human. The autophagy protein LC3, a mammalian homologue ofAtg8, was originally identified as microtubule-associated protein 1 light chain 3. It is acomponent of both the MAP1A and MAP1B microtubule-binding domains and theheavy-chain independent regulation of LC3 expression might modify MAP1microtubule-binding activity during development. LC3 is the only known mammalianprotein identified that stably associates with the autophagosome membranes. LC3-I iscytosolic and LC3-II is membrane bound and enriched in the autophagic vacuolefraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker fordistinguishing autophagy in mammalian cells. Alternate Names: anti-Microtubule-associated proteins 1A/1B light chain 3B MAP1A/MAP1B LC3 Bantibody, anti-MAP1A/b light chain 3 B antibody, anti-Autophagy-related protein LC3 Bantibody, anti-Autophagy-related ubiquitin-like modifier LC3 B antibody.
Gene Symbol: MAP1LC3A
Gene ID 84557, 66734, 64862
UniProt Q9GZQ8
Research Area Cancer, Autophagy, Cytoskeleton
Application Notes This LC3 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence (PMID 21545732), Immunoprecipitation and Immunohistochemistry paraffin embedded sections. By Western blot bands are seen at ~19 kDa, representing LC3-I, and ~17 kDa, representing LC3-II. In ICC/IF, cytoplasmic staining was observed in HeLa cells.Use in FLOW cytometry reported in scientific literature ( PMID 24419333)
Recommended dilutions: Immunohistochemistry 1:200-1:400, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin 1:200-1:400, Immunoprecipitation 20 µg / 500 µg of lysate, Western Blot 2 µg/mL
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Preservative Without preservative
Storage 4 °C
Storage Comment 4 °C in the dark
Supplier Images
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 25-121) antibody (Biotin) anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 25-121) antibody (Biotin)
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 25-121) antibody (Biotin) (2) Staining of human brain, cerebral cortex, cell crocesses in gray matter using ABIN250719.
General Kabeya, Mizushima, Ueno et al.: "LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing." in: The EMBO journal, Vol. 19, Issue 21, pp. 5720-8, 2000 (PubMed).

Mizushima, Yamamoto, Hatano et al.: "Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells." in: The Journal of cell biology, Vol. 152, Issue 4, pp. 657-68, 2001 (PubMed).

Zhang, Yu, Pan et al.: "Small molecule regulators of autophagy identified by an image-based high-throughput screen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 48, pp. 19023-8, 2007 (PubMed).

Lee, Cao, Mostoslavsky et al.: "A role for the NAD-dependent deacetylase Sirt1 in the regulation of autophagy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 9, pp. 3374-9, 2008 (PubMed).

Swerdlow, McColl, Rong et al.: "Apoptosis inhibition by Bcl-2 gives way to autophagy in glucocorticoid-treated lymphocytes." in: Autophagy, Vol. 4, Issue 5, pp. 612-20, 2008 (PubMed).

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